Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia
Background: Periodontitis is a chronic bacterial infection of tooth that increases the risk of systemic diseases like diabetes, cancer, and cardiovascular diseases. Although it has been have found altered lipids in periodontitis patients, its gene regulation is largely unknown. This study aimed to e...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723001696 |
| _version_ | 1797804643639623680 |
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| author | Paladugu Devi Navya Gurumoorthy Kaarthikeyan Ahmed Alamoudi Maha A Bahammam Samar Saeed Khan Khalid J. Alzahrani Ibrahim F. Halawani Fuad M. Alzahrani Khalaf F Alsharif A.Thirumal Raj Hosam Ali Baeshen Shankargouda Patil |
| author_facet | Paladugu Devi Navya Gurumoorthy Kaarthikeyan Ahmed Alamoudi Maha A Bahammam Samar Saeed Khan Khalid J. Alzahrani Ibrahim F. Halawani Fuad M. Alzahrani Khalaf F Alsharif A.Thirumal Raj Hosam Ali Baeshen Shankargouda Patil |
| author_sort | Paladugu Devi Navya |
| collection | DOAJ |
| description | Background: Periodontitis is a chronic bacterial infection of tooth that increases the risk of systemic diseases like diabetes, cancer, and cardiovascular diseases. Although it has been have found altered lipids in periodontitis patients, its gene regulation is largely unknown. This study aimed to examine the lipid meditated regulatory network in periodontitis that may helpful in early detection of periodontal mediated systemic diseases. Methods: We employed a high-throughput gene expression data of 1) patients with periodontitis (n = 6); 2) the periodontitis patients with dyslipidemia (n = 6); and 3) healthy control group (n = 6). The over represented (DEGs) genes in SET-A (control vs. periodontitis) and SET-B (control vs. periodontitis with dyslipidemia) was identified. The protein interaction network was generated for the over represented genes in both the conditions. The constructed network was dissected into multiple regulatory clusters, containing over expressed transcription factors with its interacting proteins. Further the behavior of the clusters was determined through gene ontology and molecular pathways. Results: On expression analysis, 751 in SET-A and 561 in SET-B were over expressed compared to healthy control. Using over expressed genes, protein interaction networks were constructed for SET-A and SET-B, respectively. Topological analysis revel the difference in the complexity of both the network. Four regulatory clusters (ESR1, FOS, RUNX2, and SP1) from SET-A and six (ESR1, ESR2, FOS, JDP2, PBX1, and TAL1) from the SET-B network was extracted. Each cluster displayed a variety of molecular mechanism associated with immune system, cell cycle, and signal processing. Clusters from SET-B showed diverse regulatory pattern in associated with cancer, neurological, psychiatric and metabolic diseases. Conclusion: Our findings demonstrate difference in regulatory patterns between periodontitis and periodontitis with dyslipidemia. These finding may provide evidence for dyslipidemia mediated periodontitis contribute to progressive systemic diseases. Further experiments are required to validate these regulators as biomarkers and drug targets. |
| first_indexed | 2024-03-13T05:39:13Z |
| format | Article |
| id | doaj.art-0e2bd0d7066e46c78def63226e292dd9 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-13T05:39:13Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-0e2bd0d7066e46c78def63226e292dd92023-06-14T04:32:54ZengElsevierJournal of King Saud University: Science1018-36472023-07-01355102707Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemiaPaladugu Devi Navya0Gurumoorthy Kaarthikeyan1Ahmed Alamoudi2Maha A Bahammam3Samar Saeed Khan4Khalid J. Alzahrani5Ibrahim F. Halawani6Fuad M. Alzahrani7Khalaf F Alsharif8A.Thirumal Raj9Hosam Ali Baeshen10Shankargouda Patil11Department of Periodontics, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600 077, IndiaDepartment of Biochemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600 077, IndiaOral Biology Department, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Periodontology, Faculty of Dentistry, King Abdulaziz University, Jeddah 22254, Saudi Arabia; Executive Presidency of Academic Affairs, Saudi Commission for Health Specialties, Riyadh 11614, Saudi ArabiaDepartment of Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai 600130, IndiaDepartment of Orthodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCollege of Dental Medicine, Roseman University OF Health Sciences, South Jordan UTAH- 84095, USA; Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600077, India; Corresponding author at: College of Dental medicine, Roseman University OF Health Sciences, South Jordan UTAH- 84095, USA.Background: Periodontitis is a chronic bacterial infection of tooth that increases the risk of systemic diseases like diabetes, cancer, and cardiovascular diseases. Although it has been have found altered lipids in periodontitis patients, its gene regulation is largely unknown. This study aimed to examine the lipid meditated regulatory network in periodontitis that may helpful in early detection of periodontal mediated systemic diseases. Methods: We employed a high-throughput gene expression data of 1) patients with periodontitis (n = 6); 2) the periodontitis patients with dyslipidemia (n = 6); and 3) healthy control group (n = 6). The over represented (DEGs) genes in SET-A (control vs. periodontitis) and SET-B (control vs. periodontitis with dyslipidemia) was identified. The protein interaction network was generated for the over represented genes in both the conditions. The constructed network was dissected into multiple regulatory clusters, containing over expressed transcription factors with its interacting proteins. Further the behavior of the clusters was determined through gene ontology and molecular pathways. Results: On expression analysis, 751 in SET-A and 561 in SET-B were over expressed compared to healthy control. Using over expressed genes, protein interaction networks were constructed for SET-A and SET-B, respectively. Topological analysis revel the difference in the complexity of both the network. Four regulatory clusters (ESR1, FOS, RUNX2, and SP1) from SET-A and six (ESR1, ESR2, FOS, JDP2, PBX1, and TAL1) from the SET-B network was extracted. Each cluster displayed a variety of molecular mechanism associated with immune system, cell cycle, and signal processing. Clusters from SET-B showed diverse regulatory pattern in associated with cancer, neurological, psychiatric and metabolic diseases. Conclusion: Our findings demonstrate difference in regulatory patterns between periodontitis and periodontitis with dyslipidemia. These finding may provide evidence for dyslipidemia mediated periodontitis contribute to progressive systemic diseases. Further experiments are required to validate these regulators as biomarkers and drug targets.http://www.sciencedirect.com/science/article/pii/S1018364723001696DyslipidemiaPeriodontitisSystemic diseases |
| spellingShingle | Paladugu Devi Navya Gurumoorthy Kaarthikeyan Ahmed Alamoudi Maha A Bahammam Samar Saeed Khan Khalid J. Alzahrani Ibrahim F. Halawani Fuad M. Alzahrani Khalaf F Alsharif A.Thirumal Raj Hosam Ali Baeshen Shankargouda Patil Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia Journal of King Saud University: Science Dyslipidemia Periodontitis Systemic diseases |
| title | Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia |
| title_full | Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia |
| title_fullStr | Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia |
| title_full_unstemmed | Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia |
| title_short | Transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia |
| title_sort | transcriptional regulatory signatures of systemic diseases in periodontitis with dyslipidemia |
| topic | Dyslipidemia Periodontitis Systemic diseases |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723001696 |
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