AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS
Infectious bronchitis virus (IBV), a gamma coronavirus, is responsible for a highly contagious disease in chickens resulting in substantial economic losses to the poultry industry around the world. The emergence of new types of IBVs is considered one of the major concerns regarding to IB. A prelimi...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2021-12-01
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| Series: | Slovenian Veterinary Research |
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| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/1432 |
| _version_ | 1797224986411270144 |
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| author | Ali Mahmoud Reham ElBakrey Shimaa Mansour Amal Eid |
| author_facet | Ali Mahmoud Reham ElBakrey Shimaa Mansour Amal Eid |
| author_sort | Ali Mahmoud |
| collection | DOAJ |
| description |
Infectious bronchitis virus (IBV), a gamma coronavirus, is responsible for a highly contagious disease in chickens resulting in substantial economic losses to the poultry industry around the world. The emergence of new types of IBVs is considered one of the major concerns regarding to IB. A preliminary diagnosis of IB was made based on the flock history and the characteristic pathologic findings. In the cur-rent study, we investigated the outbreaks of respiratory signs and diarrhea in broiler chickens (n=77) be-tween 2014 and 2018. Upon necropsy, the affected chickens showed tracheal and kidney lesions, sugges-tive of IBV. The IBV was detected in 33 farms using real-time reverse transcription-PCR, targeting the nu-cleocapsid gene. Investigation of IBV among vaccinated chickens would considerably ameliorate the pre-sent immunization programs and support the development of more effective vaccines. The IBV was de-tected in vaccinated birds (45.8%). The virus was isolated in fertile chicken eggs, which revealed embry-onic mortality (20-40%) within 5-7 days after the 3rd passage, small embryos or dwarfing up to 30.2% versus non-inoculated ones. Virus pathogenicity was determined experimentally in 7-day-old broiler chickens inoculated oculo-nasally with 104 median embryo infective dose. The infected birds experienced severe respiratory signs, diarrhea and tracheal and renal lesions. The virus displayed a wide range of tissue tropism, particularly in the trachea, lungs and kidneys, and was shed via the respiratory tract and cloaca. The results emphasized the significance of conducting a comprehensive surveillance of IBV infections among chicken flocks in Egypt and provide significant insights into the virulence properties of IBV. Further studies of nucleotide sequence analysis of circulating IBV strains and evaluation of vaccine efficacy are needed.
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| first_indexed | 2024-04-24T14:01:50Z |
| format | Article |
| id | doaj.art-0e3443ada7fd4d37b0841f4c23ef7341 |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:01:50Z |
| publishDate | 2021-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-0e3443ada7fd4d37b0841f4c23ef73412024-04-03T12:38:30ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612021-12-015824-Suppl10.26873/SVR-1432-2021AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENSAli Mahmoud0Reham ElBakrey1Shimaa Mansour2Amal Eid3Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt. - Veterinary Medicine Directorate, Mansoura, Dakahlia, Egypt.Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, EgyptDepartment of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt. Infectious bronchitis virus (IBV), a gamma coronavirus, is responsible for a highly contagious disease in chickens resulting in substantial economic losses to the poultry industry around the world. The emergence of new types of IBVs is considered one of the major concerns regarding to IB. A preliminary diagnosis of IB was made based on the flock history and the characteristic pathologic findings. In the cur-rent study, we investigated the outbreaks of respiratory signs and diarrhea in broiler chickens (n=77) be-tween 2014 and 2018. Upon necropsy, the affected chickens showed tracheal and kidney lesions, sugges-tive of IBV. The IBV was detected in 33 farms using real-time reverse transcription-PCR, targeting the nu-cleocapsid gene. Investigation of IBV among vaccinated chickens would considerably ameliorate the pre-sent immunization programs and support the development of more effective vaccines. The IBV was de-tected in vaccinated birds (45.8%). The virus was isolated in fertile chicken eggs, which revealed embry-onic mortality (20-40%) within 5-7 days after the 3rd passage, small embryos or dwarfing up to 30.2% versus non-inoculated ones. Virus pathogenicity was determined experimentally in 7-day-old broiler chickens inoculated oculo-nasally with 104 median embryo infective dose. The infected birds experienced severe respiratory signs, diarrhea and tracheal and renal lesions. The virus displayed a wide range of tissue tropism, particularly in the trachea, lungs and kidneys, and was shed via the respiratory tract and cloaca. The results emphasized the significance of conducting a comprehensive surveillance of IBV infections among chicken flocks in Egypt and provide significant insights into the virulence properties of IBV. Further studies of nucleotide sequence analysis of circulating IBV strains and evaluation of vaccine efficacy are needed. https://www.slovetres.si/index.php/SVR/article/view/1432IBVRT-PCRpathogenicityclinical scoringhistopathologynephropathogenic strain |
| spellingShingle | Ali Mahmoud Reham ElBakrey Shimaa Mansour Amal Eid AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS Slovenian Veterinary Research IBV RT-PCR pathogenicity clinical scoring histopathology nephropathogenic strain |
| title | AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS |
| title_full | AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS |
| title_fullStr | AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS |
| title_full_unstemmed | AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS |
| title_short | AVIAN CORONAVIRUS IN EGYPT: EPIDEMIOLOGICAL ACUITIES AND PATHOGENICITY IN COMMERCIAL BROILER CHICKENS |
| title_sort | avian coronavirus in egypt epidemiological acuities and pathogenicity in commercial broiler chickens |
| topic | IBV RT-PCR pathogenicity clinical scoring histopathology nephropathogenic strain |
| url | https://www.slovetres.si/index.php/SVR/article/view/1432 |
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