Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools
Abstract Background Gastric cancer (GC) is one of the most commonly occuring gastrointestinal tumor types, and early diagnosis and operation have a notable effect on the prognosis of patients. Although certain markers, including HER2, VEGER-2, ERCC1 and Bcl-2, have been utilized in clinical practise...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-019-5406-x |
| _version_ | 1818311742763040768 |
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| author | Jiaxin Liu Zhao Liu Xiaozhi Zhang Tuotuo Gong Demao Yao |
| author_facet | Jiaxin Liu Zhao Liu Xiaozhi Zhang Tuotuo Gong Demao Yao |
| author_sort | Jiaxin Liu |
| collection | DOAJ |
| description | Abstract Background Gastric cancer (GC) is one of the most commonly occuring gastrointestinal tumor types, and early diagnosis and operation have a notable effect on the prognosis of patients. Although certain markers, including HER2, VEGER-2, ERCC1 and Bcl-2, have been utilized in clinical practise to screen out new patients with GC, the results of using these markers remains poor. The role of olfactomedin-like 2B (OLFML2B) in GC, as a member of the olfactomedin domain-containing proteins family, is remain unclear. Methods In the present study, we assessed the expression of OLFML2B, including mRNA and protein level, by using The Cancer Genome Atlas (TCGA) database and 13 pairs of clinical samples between GC and NG tissues. The correlation between expression of OLFML2B and prognosis of GC was evaculated by the Kaplan-Meier plotter and OncoLnc online tools. In addition, mechanism analysis of OLFML2B in GC was explored thought bioinformatic tools, including cBioPortal and FunRich software. Results In our study, the mRNA expression of OLFML2B in GC both TCGA database and clinical samples was consistently revealed to be significantly higher compared with that in NG tissues (P < 0.0001 for TCGA database and P = 0.0034 for clinical samples), and high OLFML2B expression was found in 9 (69.23%) of 13 clinical GC by immunohistochemistry and was positively correlated with the depth of tumor invasion and clinical stage (TNM). In addition, the AUC for a ROC of 0.867 indicated a moderate diagnostic ability of OLFML2B for GC. Survival analysis from the Kaplan-Meier plotter (P = 2.6 × 10− 6) and OncoLnc (P = 0.00276) revealed that the high expression of OLFML2B was associated with a short overall survival. Futhermore, 5% (24/478) alterations of OLFML2B were identified from cBioPortal, and among them, missense mutation (14/478) was the primary type. The results from FunRich revealed that OLFML2B participated in mediating multiple biological processes including cell growth and maintenance, regulation of the cell cycle, apoptosis and cell communication through multiple signaling pathways including the M/G1 transition pathway, post-translational protein modification and DNA replication pre-initiation. Conclusions Taken together, it could be deduced that OLFML2B may act as an oncogene in the development of GC and the overexpression of OLFML2B in GC may be used as a novel diagnostic and prognostic target for GC. |
| first_indexed | 2024-12-13T08:06:48Z |
| format | Article |
| id | doaj.art-0e3489dfedb640f788173cd37977fd52 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-13T08:06:48Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-0e3489dfedb640f788173cd37977fd522022-12-21T23:54:17ZengBMCBMC Cancer1471-24072019-03-0119111010.1186/s12885-019-5406-xBioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing toolsJiaxin Liu0Zhao Liu1Xiaozhi Zhang2Tuotuo Gong3Demao Yao4Department of Geriatric Surgery, First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Oncology Surgery, First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Radiotherapy, First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Radiotherapy, First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Geriatric Surgery, First Affiliated Hospital of Xi’an Jiaotong UniversityAbstract Background Gastric cancer (GC) is one of the most commonly occuring gastrointestinal tumor types, and early diagnosis and operation have a notable effect on the prognosis of patients. Although certain markers, including HER2, VEGER-2, ERCC1 and Bcl-2, have been utilized in clinical practise to screen out new patients with GC, the results of using these markers remains poor. The role of olfactomedin-like 2B (OLFML2B) in GC, as a member of the olfactomedin domain-containing proteins family, is remain unclear. Methods In the present study, we assessed the expression of OLFML2B, including mRNA and protein level, by using The Cancer Genome Atlas (TCGA) database and 13 pairs of clinical samples between GC and NG tissues. The correlation between expression of OLFML2B and prognosis of GC was evaculated by the Kaplan-Meier plotter and OncoLnc online tools. In addition, mechanism analysis of OLFML2B in GC was explored thought bioinformatic tools, including cBioPortal and FunRich software. Results In our study, the mRNA expression of OLFML2B in GC both TCGA database and clinical samples was consistently revealed to be significantly higher compared with that in NG tissues (P < 0.0001 for TCGA database and P = 0.0034 for clinical samples), and high OLFML2B expression was found in 9 (69.23%) of 13 clinical GC by immunohistochemistry and was positively correlated with the depth of tumor invasion and clinical stage (TNM). In addition, the AUC for a ROC of 0.867 indicated a moderate diagnostic ability of OLFML2B for GC. Survival analysis from the Kaplan-Meier plotter (P = 2.6 × 10− 6) and OncoLnc (P = 0.00276) revealed that the high expression of OLFML2B was associated with a short overall survival. Futhermore, 5% (24/478) alterations of OLFML2B were identified from cBioPortal, and among them, missense mutation (14/478) was the primary type. The results from FunRich revealed that OLFML2B participated in mediating multiple biological processes including cell growth and maintenance, regulation of the cell cycle, apoptosis and cell communication through multiple signaling pathways including the M/G1 transition pathway, post-translational protein modification and DNA replication pre-initiation. Conclusions Taken together, it could be deduced that OLFML2B may act as an oncogene in the development of GC and the overexpression of OLFML2B in GC may be used as a novel diagnostic and prognostic target for GC.http://link.springer.com/article/10.1186/s12885-019-5406-xOLFML2BGastric cancerBioinformaticDiagnosisPrognosis |
| spellingShingle | Jiaxin Liu Zhao Liu Xiaozhi Zhang Tuotuo Gong Demao Yao Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools BMC Cancer OLFML2B Gastric cancer Bioinformatic Diagnosis Prognosis |
| title | Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools |
| title_full | Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools |
| title_fullStr | Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools |
| title_full_unstemmed | Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools |
| title_short | Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools |
| title_sort | bioinformatic exploration of olfml2b overexpression in gastric cancer base on multiple analyzing tools |
| topic | OLFML2B Gastric cancer Bioinformatic Diagnosis Prognosis |
| url | http://link.springer.com/article/10.1186/s12885-019-5406-x |
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