Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007.
Global dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive respo...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667626/pdf/?tool=EBI |
| _version_ | 1818459808530956288 |
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| author | Christian Julian Villabona-Arenas Adriano Mondini Irene Bosch Diane J Schimdt Carlos E Calzavara-Silva Paolo M de A Zanotto Maurício L Nogueira |
| author_facet | Christian Julian Villabona-Arenas Adriano Mondini Irene Bosch Diane J Schimdt Carlos E Calzavara-Silva Paolo M de A Zanotto Maurício L Nogueira |
| author_sort | Christian Julian Villabona-Arenas |
| collection | DOAJ |
| description | Global dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive responses is important for vaccine development. The investigation of naturally occurring genomic variants may play an important role in the comprehension of different adaptive strategies used by these mutants to evade the human immune system. In order to elucidate this role we sequenced the complete polyprotein-coding region of thirty-three DENV-3 isolates to characterize variants circulating under high endemicity in the city of São José de Rio Preto, Brazil, during the onset of the 2006-07 epidemic. By inferring the evolutionary history on a local-scale and estimating rates of synonymous (dS) and nonsynonimous (dN) substitutions, we have documented at least two different introductions of DENV-3 into the city and detected 10 polymorphic codon sites under significant positive selection (dN/dS > 1) and 8 under significant purifying selection (dN/dS < 1). We found several polymorphic amino acid coding sites in the envelope (15), NS1 (17), NS2A (11), and NS5 (24) genes, which suggests that these genes may be experiencing relatively recent adaptive changes. Furthermore, some polymorphisms correlated with changes in the immunogenicity of several epitopes. Our study highlights the existence of significant and informative DENV variability at the spatio-temporal scale of an urban outbreak. |
| first_indexed | 2024-12-14T23:20:14Z |
| format | Article |
| id | doaj.art-0e39a36422e3482ca21c0456726f1e60 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T23:20:14Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0e39a36422e3482ca21c0456726f1e602022-12-21T22:43:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6349610.1371/journal.pone.0063496Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007.Christian Julian Villabona-ArenasAdriano MondiniIrene BoschDiane J SchimdtCarlos E Calzavara-SilvaPaolo M de A ZanottoMaurício L NogueiraGlobal dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive responses is important for vaccine development. The investigation of naturally occurring genomic variants may play an important role in the comprehension of different adaptive strategies used by these mutants to evade the human immune system. In order to elucidate this role we sequenced the complete polyprotein-coding region of thirty-three DENV-3 isolates to characterize variants circulating under high endemicity in the city of São José de Rio Preto, Brazil, during the onset of the 2006-07 epidemic. By inferring the evolutionary history on a local-scale and estimating rates of synonymous (dS) and nonsynonimous (dN) substitutions, we have documented at least two different introductions of DENV-3 into the city and detected 10 polymorphic codon sites under significant positive selection (dN/dS > 1) and 8 under significant purifying selection (dN/dS < 1). We found several polymorphic amino acid coding sites in the envelope (15), NS1 (17), NS2A (11), and NS5 (24) genes, which suggests that these genes may be experiencing relatively recent adaptive changes. Furthermore, some polymorphisms correlated with changes in the immunogenicity of several epitopes. Our study highlights the existence of significant and informative DENV variability at the spatio-temporal scale of an urban outbreak.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667626/pdf/?tool=EBI |
| spellingShingle | Christian Julian Villabona-Arenas Adriano Mondini Irene Bosch Diane J Schimdt Carlos E Calzavara-Silva Paolo M de A Zanotto Maurício L Nogueira Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. PLoS ONE |
| title | Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. |
| title_full | Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. |
| title_fullStr | Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. |
| title_full_unstemmed | Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. |
| title_short | Dengue virus type 3 adaptive changes during epidemics in São Jose de Rio Preto, Brazil, 2006-2007. |
| title_sort | dengue virus type 3 adaptive changes during epidemics in sao jose de rio preto brazil 2006 2007 |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667626/pdf/?tool=EBI |
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