Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer
Using a topical formulation of 5-aminolevulinic acid (ALA), a mediated photodynamic therapy (PDT) was developed to treat oral precancerous and cancerous lesions. Several clinical results demonstrated that this special ALA preparation containing Carbopol 971P (CP971P), a bioadhesive polymer, resulted...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-03-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790210600050 |
| _version_ | 1818142146602991616 |
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| author | Shu-Fang Chang Yu-Tsai Yang Wei-Ling Li Che-Tong Lin Tsuimin Tsai |
| author_facet | Shu-Fang Chang Yu-Tsai Yang Wei-Ling Li Che-Tong Lin Tsuimin Tsai |
| author_sort | Shu-Fang Chang |
| collection | DOAJ |
| description | Using a topical formulation of 5-aminolevulinic acid (ALA), a mediated photodynamic therapy (PDT) was developed to treat oral precancerous and cancerous lesions. Several clinical results demonstrated that this special ALA preparation containing Carbopol 971P (CP971P), a bioadhesive polymer, resulted in a satisfactory PDT effect. It is believed that this PDT efficacy may be due to the increased bioadhesion of the preparation at the treatment site. We investigated whether there was a beneficial effect of CP971P on ALA PDT in vitro.
Materials and methods: CP971P was co-incubated with ALA in a cell culture system. The effect of CP971P was evaluated by monitoring the uptake of ALA, the fluorescence intensity of protoporphyrin IX, and the cell survival rate.
Results: The polymer was found to enhance the fluorescence intensity of protoporphyrin IX and the subsequent PDT effect when concurrently dosed with ALA.
Conclusion: Our results showed that a formulation of ALA containing C971P could provide a better ALA PDT effect in cancer treatment. |
| first_indexed | 2024-12-11T11:11:08Z |
| format | Article |
| id | doaj.art-0e3a9f0f0ef6447497c9d5c1a72d1b7b |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-11T11:11:08Z |
| publishDate | 2010-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-0e3a9f0f0ef6447497c9d5c1a72d1b7b2022-12-22T01:09:32ZengElsevierJournal of Dental Sciences1991-79022010-03-0151303510.1016/S1991-7902(10)60005-0Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymerShu-Fang Chang0Yu-Tsai Yang1Wei-Ling Li2Che-Tong Lin3Tsuimin Tsai4Department of Dentistry, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, TaiwanCollege of Oral Medicine, Taipei Medical University, Taipei, TaiwanGraduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, TaiwanCollege of Oral Medicine, Taipei Medical University, Taipei, TaiwanCollege of Oral Medicine, Taipei Medical University, Taipei, TaiwanUsing a topical formulation of 5-aminolevulinic acid (ALA), a mediated photodynamic therapy (PDT) was developed to treat oral precancerous and cancerous lesions. Several clinical results demonstrated that this special ALA preparation containing Carbopol 971P (CP971P), a bioadhesive polymer, resulted in a satisfactory PDT effect. It is believed that this PDT efficacy may be due to the increased bioadhesion of the preparation at the treatment site. We investigated whether there was a beneficial effect of CP971P on ALA PDT in vitro. Materials and methods: CP971P was co-incubated with ALA in a cell culture system. The effect of CP971P was evaluated by monitoring the uptake of ALA, the fluorescence intensity of protoporphyrin IX, and the cell survival rate. Results: The polymer was found to enhance the fluorescence intensity of protoporphyrin IX and the subsequent PDT effect when concurrently dosed with ALA. Conclusion: Our results showed that a formulation of ALA containing C971P could provide a better ALA PDT effect in cancer treatment.http://www.sciencedirect.com/science/article/pii/S1991790210600050aminolevulinic acidCarbopolphotodynamic therapyprotoporphyrin IX |
| spellingShingle | Shu-Fang Chang Yu-Tsai Yang Wei-Ling Li Che-Tong Lin Tsuimin Tsai Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer Journal of Dental Sciences aminolevulinic acid Carbopol photodynamic therapy protoporphyrin IX |
| title | Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer |
| title_full | Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer |
| title_fullStr | Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer |
| title_full_unstemmed | Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer |
| title_short | Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer |
| title_sort | enhancement of 5 aminolevulinic acid induced photodynamic therapy by a bioadhesive polymer |
| topic | aminolevulinic acid Carbopol photodynamic therapy protoporphyrin IX |
| url | http://www.sciencedirect.com/science/article/pii/S1991790210600050 |
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