Study on relationship between genetic abnormalities and clinicopathological features in K hospital's patients with colorectal cancer
The MAPK-ERK, as well as PI3K-AKT signaling transduction pathway, represents a pivotal function in tumorigenesis. Genetic alterations of potential tumor-driven genes, for instance, KRAS, BRAF, NRAS, and PIK3CA can result in uncontrolled cell proliferation and progression. The main aims of the study...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2022-08-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=23665 |
| _version_ | 1811235330021392384 |
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| author | Linh Dieu Vuong Ha Hoang Chu Quang Ngoc Nguyen |
| author_facet | Linh Dieu Vuong Ha Hoang Chu Quang Ngoc Nguyen |
| author_sort | Linh Dieu Vuong |
| collection | DOAJ |
| description | The MAPK-ERK, as well as PI3K-AKT signaling transduction pathway, represents a pivotal function in tumorigenesis. Genetic alterations of potential tumor-driven genes, for instance, KRAS, BRAF, NRAS, and PIK3CA can result in uncontrolled cell proliferation and progression. The main aims of the study were not only to identify the prevalence of KRAS, BRAF, NRAS, PIK3CA molecular modifications but also to evaluate the relationship between gene changes and clinical and/or pathological characteristics of 251 Vietnamese colorectal cancer. Genetic abnormalities on KRAS, BRAF, NRAS, and PIK3CA were detected through the utility of Realtime PCR, Pyrosequencing, and Direct sequencing methods, respectively. The frequency of KRAS, BRAF, NRAS, and PIK3CA mutations were 34.3%, 6.4%, 7.2%, and 17.5%, in turn. KRAS mutation was mutually exclusive against that of NRAS and BRAF mutations in CRC. BRAF, as well as RAS/RAF mutations, were more usual in older age. A significant association between PIK3CA mutations and age together with differentiation of CRC was determined. In addition, PIK3CA mutation tended to coexist with KRAS but not with NRAS and BRAF mutation. Our results indicate the information of molecular markers that contribute to self-sufficient oncogenic mechanisms in the carcinogenesis of CRC. [ J Adv Biotechnol Exp Ther 2022; 5(2.000): 408-416] |
| first_indexed | 2024-04-12T11:49:00Z |
| format | Article |
| id | doaj.art-0e3b1e81dd804a80b6f30727d3f5bd45 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-04-12T11:49:00Z |
| publishDate | 2022-08-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-0e3b1e81dd804a80b6f30727d3f5bd452022-12-22T03:34:14ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602022-08-015240841610.5455/jabet.2022.d12423665Study on relationship between genetic abnormalities and clinicopathological features in K hospital's patients with colorectal cancerLinh Dieu Vuong0Ha Hoang Chu1Quang Ngoc Nguyen2Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam National Key Laboratory of Gene Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay, Hanoi, Vietnam Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, VietnamThe MAPK-ERK, as well as PI3K-AKT signaling transduction pathway, represents a pivotal function in tumorigenesis. Genetic alterations of potential tumor-driven genes, for instance, KRAS, BRAF, NRAS, and PIK3CA can result in uncontrolled cell proliferation and progression. The main aims of the study were not only to identify the prevalence of KRAS, BRAF, NRAS, PIK3CA molecular modifications but also to evaluate the relationship between gene changes and clinical and/or pathological characteristics of 251 Vietnamese colorectal cancer. Genetic abnormalities on KRAS, BRAF, NRAS, and PIK3CA were detected through the utility of Realtime PCR, Pyrosequencing, and Direct sequencing methods, respectively. The frequency of KRAS, BRAF, NRAS, and PIK3CA mutations were 34.3%, 6.4%, 7.2%, and 17.5%, in turn. KRAS mutation was mutually exclusive against that of NRAS and BRAF mutations in CRC. BRAF, as well as RAS/RAF mutations, were more usual in older age. A significant association between PIK3CA mutations and age together with differentiation of CRC was determined. In addition, PIK3CA mutation tended to coexist with KRAS but not with NRAS and BRAF mutation. Our results indicate the information of molecular markers that contribute to self-sufficient oncogenic mechanisms in the carcinogenesis of CRC. [ J Adv Biotechnol Exp Ther 2022; 5(2.000): 408-416]http://www.ejmanager.com/fulltextpdf.php?mno=23665krasnrasbrafpik3ca mutationscolorectal cancer (crc) |
| spellingShingle | Linh Dieu Vuong Ha Hoang Chu Quang Ngoc Nguyen Study on relationship between genetic abnormalities and clinicopathological features in K hospital's patients with colorectal cancer Journal of Advanced Biotechnology and Experimental Therapeutics kras nras braf pik3ca mutations colorectal cancer (crc) |
| title | Study on relationship between genetic abnormalities and clinicopathological features in K
hospital's patients with colorectal cancer |
| title_full | Study on relationship between genetic abnormalities and clinicopathological features in K
hospital's patients with colorectal cancer |
| title_fullStr | Study on relationship between genetic abnormalities and clinicopathological features in K
hospital's patients with colorectal cancer |
| title_full_unstemmed | Study on relationship between genetic abnormalities and clinicopathological features in K
hospital's patients with colorectal cancer |
| title_short | Study on relationship between genetic abnormalities and clinicopathological features in K
hospital's patients with colorectal cancer |
| title_sort | study on relationship between genetic abnormalities and clinicopathological features in k hospital s patients with colorectal cancer |
| topic | kras nras braf pik3ca mutations colorectal cancer (crc) |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=23665 |
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