| Summary: | Bovine viral diarrhea virus (BVDV) is a pathogen that affects ruminants worldwide and is one of the most economically important diseases
of cattle. Although BVDV infections have been increasingly reported in China, the pathogenesis and genetic characteristics of these BVDV
isolates have not been thoroughly investigated. Here, we report the identification and characterization of a novel BVDV isolate, designated
LC, which was isolated from the feces of a cattle with diarrhea. The complete genome of isolate LC was 12,271 nucleotides and contained
a 5"-UTR of 389 nucleotides, a 3"-UTR of 189 nucleotides, and a large ORF encoding a polyprotein consisting of 3898 amino acids. Genomic
comparisons and phylogenetic analyses of the complete genomic sequence clearly showed that the isolate was a BVDV-1q subtype.
Experimental infection of calves with isolate LC resulted in the development of clinical signs including elevated rectal temperatures, nasal
discharge and decreased leucopenia. Viral antigen was detected in infected animal tissues using immunohistochemistry. This is the first
report of the genomic sequence of a BVDV-1q virus isolated from cattle. The virus strain was moderately pathogenic in calves and could
potentially be used as a BVDV challenge virus to evaluate the efficacy of BVDV vaccines.
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