Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity
Bovine viral diarrhea virus (BVDV) is a pathogen that affects ruminants worldwide and is one of the most economically important diseases of cattle. Although BVDV infections have been increasingly reported in China, the pathogenesis and genetic characteristics of these BVDV isolates have not been tho...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2020-01-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2674 |
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| author | Yanhua HE Xusheng MA Xin HUANG Jinliang SHENG Fagang ZHONG Xinxia ZHAO Yunfen ZHANG Chuangfu CHEN |
| author_facet | Yanhua HE Xusheng MA Xin HUANG Jinliang SHENG Fagang ZHONG Xinxia ZHAO Yunfen ZHANG Chuangfu CHEN |
| author_sort | Yanhua HE |
| collection | DOAJ |
| description | Bovine viral diarrhea virus (BVDV) is a pathogen that affects ruminants worldwide and is one of the most economically important diseases
of cattle. Although BVDV infections have been increasingly reported in China, the pathogenesis and genetic characteristics of these BVDV
isolates have not been thoroughly investigated. Here, we report the identification and characterization of a novel BVDV isolate, designated
LC, which was isolated from the feces of a cattle with diarrhea. The complete genome of isolate LC was 12,271 nucleotides and contained
a 5"-UTR of 389 nucleotides, a 3"-UTR of 189 nucleotides, and a large ORF encoding a polyprotein consisting of 3898 amino acids. Genomic
comparisons and phylogenetic analyses of the complete genomic sequence clearly showed that the isolate was a BVDV-1q subtype.
Experimental infection of calves with isolate LC resulted in the development of clinical signs including elevated rectal temperatures, nasal
discharge and decreased leucopenia. Viral antigen was detected in infected animal tissues using immunohistochemistry. This is the first
report of the genomic sequence of a BVDV-1q virus isolated from cattle. The virus strain was moderately pathogenic in calves and could
potentially be used as a BVDV challenge virus to evaluate the efficacy of BVDV vaccines. |
| first_indexed | 2024-03-13T04:37:56Z |
| format | Article |
| id | doaj.art-0e475df0f10849fe8bc04862157f5529 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:56Z |
| publishDate | 2020-01-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-0e475df0f10849fe8bc04862157f55292023-06-19T06:32:52ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512020-01-0126337738410.9775/kvfd.2019.232732674Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicityYanhua HE0Xusheng MA1Xin HUANG2Jinliang SHENG3Fagang ZHONG4Xinxia ZHAO5Yunfen ZHANG6Chuangfu CHEN7College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, CHINAHouBo College of XinJiang Medical University, Kelemayi, Xinjiang 834000, CHINAState Key Laboratory of Sheep Genetic Improvement and Healthy Production/Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang 832000, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, CHINAState Key Laboratory of Sheep Genetic Improvement and Healthy Production/Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang 832000, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, CHINABovine viral diarrhea virus (BVDV) is a pathogen that affects ruminants worldwide and is one of the most economically important diseases of cattle. Although BVDV infections have been increasingly reported in China, the pathogenesis and genetic characteristics of these BVDV isolates have not been thoroughly investigated. Here, we report the identification and characterization of a novel BVDV isolate, designated LC, which was isolated from the feces of a cattle with diarrhea. The complete genome of isolate LC was 12,271 nucleotides and contained a 5"-UTR of 389 nucleotides, a 3"-UTR of 189 nucleotides, and a large ORF encoding a polyprotein consisting of 3898 amino acids. Genomic comparisons and phylogenetic analyses of the complete genomic sequence clearly showed that the isolate was a BVDV-1q subtype. Experimental infection of calves with isolate LC resulted in the development of clinical signs including elevated rectal temperatures, nasal discharge and decreased leucopenia. Viral antigen was detected in infected animal tissues using immunohistochemistry. This is the first report of the genomic sequence of a BVDV-1q virus isolated from cattle. The virus strain was moderately pathogenic in calves and could potentially be used as a BVDV challenge virus to evaluate the efficacy of BVDV vaccines.https://vetdergikafkas.org/pdf.php?id=2674bovine viral diarrhea virus (bvdv)genomicsgenotypingpathogenesis |
| spellingShingle | Yanhua HE Xusheng MA Xin HUANG Jinliang SHENG Fagang ZHONG Xinxia ZHAO Yunfen ZHANG Chuangfu CHEN Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity Kafkas Universitesi Veteriner Fakültesi Dergisi bovine viral diarrhea virus (bvdv) genomics genotyping pathogenesis |
| title | Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity |
| title_full | Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity |
| title_fullStr | Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity |
| title_full_unstemmed | Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity |
| title_short | Detection of bvdv 1q in china: genetic characterization and experimental infection for the investigation of it"s pathogenicity |
| title_sort | detection of bvdv 1q in china genetic characterization and experimental infection for the investigation of it s pathogenicity |
| topic | bovine viral diarrhea virus (bvdv) genomics genotyping pathogenesis |
| url | https://vetdergikafkas.org/pdf.php?id=2674 |
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