Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model
Although the global Zika epidemic in 2015–16 fueled vaccine development efforts, there is no approved Zika vaccine or treatment available to date. Current vaccine platforms in clinical trials are administered via either subcutaneous or intramuscular injections, which are painful and decrease complia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/3/583 |
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| author | Akanksha Kale Devyani Joshi Ipshita Menon Priyal Bagwe Smital Patil Sharon Vijayanand Keegan Braz Gomes Mohammad N. Uddin Martin J. D’Souza |
| author_facet | Akanksha Kale Devyani Joshi Ipshita Menon Priyal Bagwe Smital Patil Sharon Vijayanand Keegan Braz Gomes Mohammad N. Uddin Martin J. D’Souza |
| author_sort | Akanksha Kale |
| collection | DOAJ |
| description | Although the global Zika epidemic in 2015–16 fueled vaccine development efforts, there is no approved Zika vaccine or treatment available to date. Current vaccine platforms in clinical trials are administered via either subcutaneous or intramuscular injections, which are painful and decrease compliance. Therefore, in the present study, we explored Zika vaccine microparticles (MPs)-loaded dissolving microneedles (MNs) with adjuvant MPs encapsulating Alhydrogel<sup>®</sup> and MPL-A<sup>®</sup> administered via the transdermal route as a pain-free vaccine strategy. We characterized the MNs for needle length, pore formation, and dissolvability when applied to murine skin. Further, we evaluated the in vivo efficacy of vaccine MPs-loaded MNs with or without adjuvants by measuring the immune response after transdermal immunization. The vaccine MPs-loaded dissolving MNs with adjuvants induced significant IgG, IgG1, and IgG2a titers in immunized mice compared to the untreated control group. After the dosing regimen, the animals were challenged with Zika virus, monitored for seven days, and sacrificed to collect spleen and lymph nodes. The lymphocytes and splenocytes from the immunized mice showed significant expressions of helper (CD4) and cytotoxic (CD8a) cell surface markers compared to the control group. Thus, this study puts forth a ‘proof-of-concept’ for a pain-free transdermal vaccine strategy against Zika. |
| first_indexed | 2024-03-11T05:47:46Z |
| format | Article |
| id | doaj.art-0e4cf3946da64c5fa287089e80078778 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T05:47:46Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-0e4cf3946da64c5fa287089e800787782023-11-17T14:18:06ZengMDPI AGVaccines2076-393X2023-03-0111358310.3390/vaccines11030583Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine ModelAkanksha Kale0Devyani Joshi1Ipshita Menon2Priyal Bagwe3Smital Patil4Sharon Vijayanand5Keegan Braz Gomes6Mohammad N. Uddin7Martin J. D’Souza8Vaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAVaccine Nanotechnology Laboratory, Center for Drug Delivery and Research, Mercer University College of Pharmacy, Atlanta, GA 30341, USAAlthough the global Zika epidemic in 2015–16 fueled vaccine development efforts, there is no approved Zika vaccine or treatment available to date. Current vaccine platforms in clinical trials are administered via either subcutaneous or intramuscular injections, which are painful and decrease compliance. Therefore, in the present study, we explored Zika vaccine microparticles (MPs)-loaded dissolving microneedles (MNs) with adjuvant MPs encapsulating Alhydrogel<sup>®</sup> and MPL-A<sup>®</sup> administered via the transdermal route as a pain-free vaccine strategy. We characterized the MNs for needle length, pore formation, and dissolvability when applied to murine skin. Further, we evaluated the in vivo efficacy of vaccine MPs-loaded MNs with or without adjuvants by measuring the immune response after transdermal immunization. The vaccine MPs-loaded dissolving MNs with adjuvants induced significant IgG, IgG1, and IgG2a titers in immunized mice compared to the untreated control group. After the dosing regimen, the animals were challenged with Zika virus, monitored for seven days, and sacrificed to collect spleen and lymph nodes. The lymphocytes and splenocytes from the immunized mice showed significant expressions of helper (CD4) and cytotoxic (CD8a) cell surface markers compared to the control group. Thus, this study puts forth a ‘proof-of-concept’ for a pain-free transdermal vaccine strategy against Zika.https://www.mdpi.com/2076-393X/11/3/583Zikavaccinemicroparticlesmicroneedles |
| spellingShingle | Akanksha Kale Devyani Joshi Ipshita Menon Priyal Bagwe Smital Patil Sharon Vijayanand Keegan Braz Gomes Mohammad N. Uddin Martin J. D’Souza Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model Vaccines Zika vaccine microparticles microneedles |
| title | Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model |
| title_full | Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model |
| title_fullStr | Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model |
| title_full_unstemmed | Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model |
| title_short | Zika Vaccine Microparticles (MPs)-Loaded Dissolving Microneedles (MNs) Elicit a Significant Immune Response in a Pre-Clinical Murine Model |
| title_sort | zika vaccine microparticles mps loaded dissolving microneedles mns elicit a significant immune response in a pre clinical murine model |
| topic | Zika vaccine microparticles microneedles |
| url | https://www.mdpi.com/2076-393X/11/3/583 |
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