Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River
Anthropogenic activities strongly influence river habitat conditions and surrounding landscape patterns. A major challenge is to understand how these changes impact microbial community composition and structure. Here, a comprehensive analysis combining physicochemical characteristics in sediment wit...
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MDPI AG
2020-05-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/12/5/1344 |
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| author | Xiaofeng Cao Yajun Wang Yan Xu Gaoqi Duan Miansong Huang Jianfeng Peng |
| author_facet | Xiaofeng Cao Yajun Wang Yan Xu Gaoqi Duan Miansong Huang Jianfeng Peng |
| author_sort | Xiaofeng Cao |
| collection | DOAJ |
| description | Anthropogenic activities strongly influence river habitat conditions and surrounding landscape patterns. A major challenge is to understand how these changes impact microbial community composition and structure. Here, a comprehensive analysis combining physicochemical characteristics in sediment with sequencing targeting the V4 region of the 16S rRNA gene was conducted to test the hypothesis that diverse habitat conditions induce dissimilarity of microbial community composition and structure in a regulated urban river. The results suggested that observed species richness and Shannon–Wiener diversity had a decreasing variation along the land use intensified gradient, while beta diversity also revealed significant separation of microbial community structure between headwaters and urban reaches. Total nitrogen (TN), total phosphorus (TP), oxidation–reduction potential (ORP) and total organic carbon (TOC) in sediment were the dominant factors in structuring bacterial and archaeal community assemblages. Further analysis in dominant fecal-associated bacteria indicated that elevated nutrient concentrations may significantly (<i>p</i> < 0.05) increase the relative abundance of <i>Clostridium</i> and <i>Acinetobacter</i> in sediment. The findings highlight the pivotal roles of alpha diversity and fecal-associated bacteria in understanding the dynamics of microbial communities in a regulated urban river ecosystem. |
| first_indexed | 2024-03-10T19:56:06Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T19:56:06Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
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| series | Water |
| spelling | doaj.art-0e4ddc456fae48ce90a818633a415f6c2023-11-19T23:53:55ZengMDPI AGWater2073-44412020-05-01125134410.3390/w12051344Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban RiverXiaofeng Cao0Yajun Wang1Yan Xu2Gaoqi Duan3Miansong Huang4Jianfeng Peng5Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, ChinaCenter for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, ChinaCenter for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, ChinaCenter for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, ChinaNingxia Capital Sponge City Construction & Development CO., LTD, Guyuan 756000, ChinaCenter for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, ChinaAnthropogenic activities strongly influence river habitat conditions and surrounding landscape patterns. A major challenge is to understand how these changes impact microbial community composition and structure. Here, a comprehensive analysis combining physicochemical characteristics in sediment with sequencing targeting the V4 region of the 16S rRNA gene was conducted to test the hypothesis that diverse habitat conditions induce dissimilarity of microbial community composition and structure in a regulated urban river. The results suggested that observed species richness and Shannon–Wiener diversity had a decreasing variation along the land use intensified gradient, while beta diversity also revealed significant separation of microbial community structure between headwaters and urban reaches. Total nitrogen (TN), total phosphorus (TP), oxidation–reduction potential (ORP) and total organic carbon (TOC) in sediment were the dominant factors in structuring bacterial and archaeal community assemblages. Further analysis in dominant fecal-associated bacteria indicated that elevated nutrient concentrations may significantly (<i>p</i> < 0.05) increase the relative abundance of <i>Clostridium</i> and <i>Acinetobacter</i> in sediment. The findings highlight the pivotal roles of alpha diversity and fecal-associated bacteria in understanding the dynamics of microbial communities in a regulated urban river ecosystem.https://www.mdpi.com/2073-4441/12/5/1344adaptive variationsdissimilaritymicrobial communityheadwaterurban reachfecal-associated bacteria |
| spellingShingle | Xiaofeng Cao Yajun Wang Yan Xu Gaoqi Duan Miansong Huang Jianfeng Peng Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River Water adaptive variations dissimilarity microbial community headwater urban reach fecal-associated bacteria |
| title | Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River |
| title_full | Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River |
| title_fullStr | Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River |
| title_full_unstemmed | Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River |
| title_short | Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River |
| title_sort | adaptive variations of sediment microbial communities and indication of fecal associated bacteria to nutrients in a regulated urban river |
| topic | adaptive variations dissimilarity microbial community headwater urban reach fecal-associated bacteria |
| url | https://www.mdpi.com/2073-4441/12/5/1344 |
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