Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan
<i>Background and Objective</i>: <i>Helicobacter pylori</i> is a human-stomach-dwelling organism that causes many gastric illnesses, including gastritis, ulcer, and gastric cancer. The purpose of the study was to perform differential proteomic analysis on <i>H. pylori&l...
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MDPI AG
2022-08-01
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| author | Syed Ali Raza Shah Hazir Rahman Muhammad Qasim Muhammad Safwan Akram Yasemin Saygideger Nanda Puspita Burcu Saygıdeğer Demir Khalid J. Alzahrani Muhammad Fayyaz ur Rehman Fuad M. Alzahrani Mohamed A. Alblihd |
| author_facet | Syed Ali Raza Shah Hazir Rahman Muhammad Qasim Muhammad Safwan Akram Yasemin Saygideger Nanda Puspita Burcu Saygıdeğer Demir Khalid J. Alzahrani Muhammad Fayyaz ur Rehman Fuad M. Alzahrani Mohamed A. Alblihd |
| author_sort | Syed Ali Raza Shah |
| collection | DOAJ |
| description | <i>Background and Objective</i>: <i>Helicobacter pylori</i> is a human-stomach-dwelling organism that causes many gastric illnesses, including gastritis, ulcer, and gastric cancer. The purpose of the study was to perform differential proteomic analysis on <i>H. pylori</i> isolates from gastritis, ulcer, and gastric cancer patients. <i>Materials and Methods</i>: <i>H. pylori</i> was isolated from antrum and fundus biopsies obtained from patients who visited the Department of Gastroenterology. Using nano-LC-QTOF MS/MS analysis, differentially regulated proteins were identified through proteome profiling of pooled samples of <i>H. pylori</i> isolated from gastritis, ulcer, and gastric cancer patients. Antigenic scores and cellular localization of proteins were determined using additional prediction tools. <i>Results</i>: A total of 14 significantly regulated proteins were identified in <i>H. pylori</i> isolated from patients with either gastritis, ulcer, or gastric cancer. Comparative analysis of groups revealed that in the case of cancer vs. gastritis, six proteins were overexpressed, out of which two proteins, including hydrogenase maturation factor (hypA) and nucleoside diphosphate kinase (ndk) involved in bacterial colonization, were only upregulated in isolates from cancer patients. Similarly, in cancer vs. ulcer, a total of nine proteins were expressed. Sec-independent protein translocase protein (tatB), involved in protein translocation, and pseudaminic acid synthase I (pseI), involved in the synthesis of functional flagella, were upregulated in cancer, while hypA and ndk were downregulated. In ulcer vs. gastritis, eight proteins were expressed. In this group, tatB was overexpressed. A reduction in thioredoxin peroxidase (bacterioferritin co-migratory protein (bcp)) was observed in ulcer vs. gastritis and cancer vs. ulcer. <i>Conclusion</i>: Our study suggested three discrete protein signatures, hypA, tatB, and bcp, with differential expression in gastritis, ulcer, and cancer. Protein expression profiles of <i>H. pylori</i> isolated from patients with these gastric diseases will help to understand the virulence and pathogenesis of <i>H. pylori</i>. |
| first_indexed | 2024-03-09T23:14:19Z |
| format | Article |
| id | doaj.art-0e4e7635dbd6496387f2a6ff5819b6cb |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T23:14:19Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj.art-0e4e7635dbd6496387f2a6ff5819b6cb2023-11-23T17:38:52ZengMDPI AGMedicina1010-660X1648-91442022-08-01589116810.3390/medicina58091168Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest PakistanSyed Ali Raza Shah0Hazir Rahman1Muhammad Qasim2Muhammad Safwan Akram3Yasemin Saygideger4Nanda Puspita5Burcu Saygıdeğer Demir6Khalid J. Alzahrani7Muhammad Fayyaz ur Rehman8Fuad M. Alzahrani9Mohamed A. Alblihd10Department of Microbiology, Abdul Wali Khan University, Mardan 23200, KP, PakistanDepartment of Microbiology, Abdul Wali Khan University, Mardan 23200, KP, PakistanDepartment of Microbiology, Kohat University of Science and Technology, Kohat 26000, KP, PakistanNational Horizons Centre, Teesside University, Darlington DL11HG, UKDepartment of Pulmonary, Cukurova University School of Medicine, 01330 Adana, TurkeyNational Horizons Centre, Teesside University, Darlington DL11HG, UKDepartment of Biotechnology, Institute of Natural and Applied Sciences, Cukurova University, 01330 Adana, TurkeyDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaInstitute of Chemistry, University of Sargodha, Sargodha 40100, PakistanDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Medical Microbiology, College of Medicine, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia<i>Background and Objective</i>: <i>Helicobacter pylori</i> is a human-stomach-dwelling organism that causes many gastric illnesses, including gastritis, ulcer, and gastric cancer. The purpose of the study was to perform differential proteomic analysis on <i>H. pylori</i> isolates from gastritis, ulcer, and gastric cancer patients. <i>Materials and Methods</i>: <i>H. pylori</i> was isolated from antrum and fundus biopsies obtained from patients who visited the Department of Gastroenterology. Using nano-LC-QTOF MS/MS analysis, differentially regulated proteins were identified through proteome profiling of pooled samples of <i>H. pylori</i> isolated from gastritis, ulcer, and gastric cancer patients. Antigenic scores and cellular localization of proteins were determined using additional prediction tools. <i>Results</i>: A total of 14 significantly regulated proteins were identified in <i>H. pylori</i> isolated from patients with either gastritis, ulcer, or gastric cancer. Comparative analysis of groups revealed that in the case of cancer vs. gastritis, six proteins were overexpressed, out of which two proteins, including hydrogenase maturation factor (hypA) and nucleoside diphosphate kinase (ndk) involved in bacterial colonization, were only upregulated in isolates from cancer patients. Similarly, in cancer vs. ulcer, a total of nine proteins were expressed. Sec-independent protein translocase protein (tatB), involved in protein translocation, and pseudaminic acid synthase I (pseI), involved in the synthesis of functional flagella, were upregulated in cancer, while hypA and ndk were downregulated. In ulcer vs. gastritis, eight proteins were expressed. In this group, tatB was overexpressed. A reduction in thioredoxin peroxidase (bacterioferritin co-migratory protein (bcp)) was observed in ulcer vs. gastritis and cancer vs. ulcer. <i>Conclusion</i>: Our study suggested three discrete protein signatures, hypA, tatB, and bcp, with differential expression in gastritis, ulcer, and cancer. Protein expression profiles of <i>H. pylori</i> isolated from patients with these gastric diseases will help to understand the virulence and pathogenesis of <i>H. pylori</i>.https://www.mdpi.com/1648-9144/58/9/1168<i>H. pylori</i>proteomicsgastric patientsmass spectrometry |
| spellingShingle | Syed Ali Raza Shah Hazir Rahman Muhammad Qasim Muhammad Safwan Akram Yasemin Saygideger Nanda Puspita Burcu Saygıdeğer Demir Khalid J. Alzahrani Muhammad Fayyaz ur Rehman Fuad M. Alzahrani Mohamed A. Alblihd Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan Medicina <i>H. pylori</i> proteomics gastric patients mass spectrometry |
| title | Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan |
| title_full | Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan |
| title_fullStr | Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan |
| title_full_unstemmed | Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan |
| title_short | Differential Proteomics of <i>Helicobacter pylori</i> Isolates from Gastritis, Ulcer, and Cancer Patients: First Study from Northwest Pakistan |
| title_sort | differential proteomics of i helicobacter pylori i isolates from gastritis ulcer and cancer patients first study from northwest pakistan |
| topic | <i>H. pylori</i> proteomics gastric patients mass spectrometry |
| url | https://www.mdpi.com/1648-9144/58/9/1168 |
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