Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite

Abstract A method with high sensitivity, good accuracy and fast response is of ever increasing importance for the simultaneous detection of AA, DA and UA. In this paper, a simple and sensitive electrochemical sensor, which based on the polyvinylpyrrolidone (PVP)-graphene composite film modified glas...

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Main Authors: Yiyong Wu, Peihong Deng, Yaling Tian, Jinxia Feng, Jingyun Xiao, Junhua Li, Jun Liu, Guangli Li, Quanguo He
Format: Article
Language:English
Published: BMC 2020-08-01
Series:Journal of Nanobiotechnology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12951-020-00672-9
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author Yiyong Wu
Peihong Deng
Yaling Tian
Jinxia Feng
Jingyun Xiao
Junhua Li
Jun Liu
Guangli Li
Quanguo He
author_facet Yiyong Wu
Peihong Deng
Yaling Tian
Jinxia Feng
Jingyun Xiao
Junhua Li
Jun Liu
Guangli Li
Quanguo He
author_sort Yiyong Wu
collection DOAJ
description Abstract A method with high sensitivity, good accuracy and fast response is of ever increasing importance for the simultaneous detection of AA, DA and UA. In this paper, a simple and sensitive electrochemical sensor, which based on the polyvinylpyrrolidone (PVP)-graphene composite film modified glassy carbon electrode (PVP-GR/GCE), was presented for detecting ascorbic acid (AA), dopamine (DA) and uric acid (UA) simultaneously. The PVP-GR/GCE has excellent electrocatalytic activity for the oxidation of AA, DA and UA. The second-order derivative linear sweep voltammetry was used for the electrochemical measurements. The peak potential differences of DA-AA, DA-UA, and UA-AA (measured on the PVP-GR/GCE) were 212, 130 and 342 mV respectively. Besides, the over potential of AA, DA and UA reduced obviously, so did the peak current increase. Under the optimum conditions, the linear ranges of AA, DA and UA were 4.0 μM–1.0 mM, 0.02–100 μM, and 0.04–100 μM, respectively. The detection limits were 0.8 μM, 0.002 μM and 0.02 μM for AA, DA, and UA. The electrochemical sensor presented the advantages of high sensitivity and selectivity, excellent reproducibility and long-term stability. Furthermore, the sensor was successfully applied to the analysis of real samples.
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spelling doaj.art-0e4ea66255e54e7a99009b5c88678c112022-12-22T03:59:50ZengBMCJournal of Nanobiotechnology1477-31552020-08-0118111310.1186/s12951-020-00672-9Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene compositeYiyong Wu0Peihong Deng1Yaling Tian2Jinxia Feng3Jingyun Xiao4Junhua Li5Jun Liu6Guangli Li7Quanguo He8School of Life Science and Chemistry, Hunan University of TechnologyKey Laboratory of Functional Metal–Organic Compounds of Hunan Province; Key Laboratory of Functional Organometallic Materials of Hunan Provincial Universities, Department of Chemistry and Material Science, Hengyang Normal UniversitySchool of Life Science and Chemistry, Hunan University of TechnologySchool of Life Science and Chemistry, Hunan University of TechnologySchool of Life Science and Chemistry, Hunan University of TechnologyKey Laboratory of Functional Metal–Organic Compounds of Hunan Province; Key Laboratory of Functional Organometallic Materials of Hunan Provincial Universities, Department of Chemistry and Material Science, Hengyang Normal UniversitySchool of Life Science and Chemistry, Hunan University of TechnologySchool of Life Science and Chemistry, Hunan University of TechnologySchool of Life Science and Chemistry, Hunan University of TechnologyAbstract A method with high sensitivity, good accuracy and fast response is of ever increasing importance for the simultaneous detection of AA, DA and UA. In this paper, a simple and sensitive electrochemical sensor, which based on the polyvinylpyrrolidone (PVP)-graphene composite film modified glassy carbon electrode (PVP-GR/GCE), was presented for detecting ascorbic acid (AA), dopamine (DA) and uric acid (UA) simultaneously. The PVP-GR/GCE has excellent electrocatalytic activity for the oxidation of AA, DA and UA. The second-order derivative linear sweep voltammetry was used for the electrochemical measurements. The peak potential differences of DA-AA, DA-UA, and UA-AA (measured on the PVP-GR/GCE) were 212, 130 and 342 mV respectively. Besides, the over potential of AA, DA and UA reduced obviously, so did the peak current increase. Under the optimum conditions, the linear ranges of AA, DA and UA were 4.0 μM–1.0 mM, 0.02–100 μM, and 0.04–100 μM, respectively. The detection limits were 0.8 μM, 0.002 μM and 0.02 μM for AA, DA, and UA. The electrochemical sensor presented the advantages of high sensitivity and selectivity, excellent reproducibility and long-term stability. Furthermore, the sensor was successfully applied to the analysis of real samples.http://link.springer.com/article/10.1186/s12951-020-00672-9Simultaneous determinationPolyvinylpyrrolidoneAscorbic acidDopamineUric acidElectrochemical sensor
spellingShingle Yiyong Wu
Peihong Deng
Yaling Tian
Jinxia Feng
Jingyun Xiao
Junhua Li
Jun Liu
Guangli Li
Quanguo He
Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite
Journal of Nanobiotechnology
Simultaneous determination
Polyvinylpyrrolidone
Ascorbic acid
Dopamine
Uric acid
Electrochemical sensor
title Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite
title_full Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite
title_fullStr Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite
title_full_unstemmed Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite
title_short Simultaneous and sensitive determination of ascorbic acid, dopamine and uric acid via an electrochemical sensor based on PVP-graphene composite
title_sort simultaneous and sensitive determination of ascorbic acid dopamine and uric acid via an electrochemical sensor based on pvp graphene composite
topic Simultaneous determination
Polyvinylpyrrolidone
Ascorbic acid
Dopamine
Uric acid
Electrochemical sensor
url http://link.springer.com/article/10.1186/s12951-020-00672-9
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