Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells
Objective: In the recent years, mesenchymal stem cells (MSCs) were considered as the suitable source of cells for transplantation into the damaged tissues in regenerative medicine. There was low number of these cells in different organs and this characteristic was the main drawback to use them in...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2021-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/fulltext/the-correlation-of-tcf4-gsk-tert-and-terc-expression-with-the-proliferation-potential-of-early-and-late-culture-of-human-mesenchymal-stem-cells-derived-from-peripheral-blood.pdf |
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| author | Zahra Fazeli Masoumeh Rajabibazl Sepideh Faramarzi Mir Davood Omrani Sayyed Mohammad Hossein Ghaderian Niloufar Safavi Naini |
| author_facet | Zahra Fazeli Masoumeh Rajabibazl Sepideh Faramarzi Mir Davood Omrani Sayyed Mohammad Hossein Ghaderian Niloufar Safavi Naini |
| author_sort | Zahra Fazeli |
| collection | DOAJ |
| description | Objective: In the recent years, mesenchymal stem cells (MSCs) were considered as the suitable source of cells for
transplantation into the damaged tissues in regenerative medicine. There was low number of these cells in different
organs and this characteristic was the main drawback to use them in treatment of diseases. Cellular senescence of the
stem cells has been demonstrated to be dependent to the telomerase activity. The aim of present experimental study
was to evaluate correlation of the expression of telomerase components and WNT signaling pathway in MSCs derived
from human peripheral blood (PB-MSCs).
Materials and Methods: In this experimental study, following the isolation of MSCs from peripheral blood mononuclear
cells, RNA was extracted from these cells in the early culture (8-9th days) and late culture (14-17th days). Then, expression
of TERT, TERC, TCF4, GSK and CTNNB1 was determined by quantitative reverse transcription polymerase chain
reaction (qRT-PCR) based on SYBR Green.
Results: Our data indicated that there was a significantly reduced expression of TERT in the late culture of human
MSCs derived from peripheral blood (P<0.05). Although a negative correlation was observed between GSK and TERC
expression levels in the early culture of MSCs, spearman analysis showed that there was no significant correlation
between the expression of telomerase components (TERC and TERT) and WNT signaling pathway (P>0.05).
Conclusion: The obtained results suggested that WNT signaling pathway likely plays a minor role in the maintenance
of telomere length and proliferation potential of MSCs. |
| first_indexed | 2024-12-19T15:41:03Z |
| format | Article |
| id | doaj.art-0e509cd3b23a4f69bf1baaf91ebee5d0 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-19T15:41:03Z |
| publishDate | 2021-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-0e509cd3b23a4f69bf1baaf91ebee5d02022-12-21T20:15:28ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142021-01-0122443143610.22074/cellj.2021.6920Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem CellsZahra Fazeli0Masoumeh Rajabibazl1Sepideh Faramarzi2Mir Davood Omrani3Sayyed Mohammad Hossein Ghaderian4Niloufar Safavi Naini5Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran,Email:z.fazeli@sbmu.ac.irDepartment of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranObjective: In the recent years, mesenchymal stem cells (MSCs) were considered as the suitable source of cells for transplantation into the damaged tissues in regenerative medicine. There was low number of these cells in different organs and this characteristic was the main drawback to use them in treatment of diseases. Cellular senescence of the stem cells has been demonstrated to be dependent to the telomerase activity. The aim of present experimental study was to evaluate correlation of the expression of telomerase components and WNT signaling pathway in MSCs derived from human peripheral blood (PB-MSCs). Materials and Methods: In this experimental study, following the isolation of MSCs from peripheral blood mononuclear cells, RNA was extracted from these cells in the early culture (8-9th days) and late culture (14-17th days). Then, expression of TERT, TERC, TCF4, GSK and CTNNB1 was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) based on SYBR Green. Results: Our data indicated that there was a significantly reduced expression of TERT in the late culture of human MSCs derived from peripheral blood (P<0.05). Although a negative correlation was observed between GSK and TERC expression levels in the early culture of MSCs, spearman analysis showed that there was no significant correlation between the expression of telomerase components (TERC and TERT) and WNT signaling pathway (P>0.05). Conclusion: The obtained results suggested that WNT signaling pathway likely plays a minor role in the maintenance of telomere length and proliferation potential of MSCs.https://celljournal.org/journal/article/fulltext/the-correlation-of-tcf4-gsk-tert-and-terc-expression-with-the-proliferation-potential-of-early-and-late-culture-of-human-mesenchymal-stem-cells-derived-from-peripheral-blood.pdfcellular senescencemesenchymal stem cellsregenerative medicinewnt signaling pathway |
| spellingShingle | Zahra Fazeli Masoumeh Rajabibazl Sepideh Faramarzi Mir Davood Omrani Sayyed Mohammad Hossein Ghaderian Niloufar Safavi Naini Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells Cell Journal cellular senescence mesenchymal stem cells regenerative medicine wnt signaling pathway |
| title | Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells |
| title_full | Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells |
| title_fullStr | Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells |
| title_full_unstemmed | Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells |
| title_short | Correlation Of TCF4, GSK, TERT And TERC Expressions With Proliferation Potential Of Early And Late Culture Of Human Peripheral Blood Mesenchymal Stem Cells |
| title_sort | correlation of tcf4 gsk tert and terc expressions with proliferation potential of early and late culture of human peripheral blood mesenchymal stem cells |
| topic | cellular senescence mesenchymal stem cells regenerative medicine wnt signaling pathway |
| url | https://celljournal.org/journal/article/fulltext/the-correlation-of-tcf4-gsk-tert-and-terc-expression-with-the-proliferation-potential-of-early-and-late-culture-of-human-mesenchymal-stem-cells-derived-from-peripheral-blood.pdf |
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