Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice
Introduction: Drug-induced liver injury is the most common cause of acute liver failure. Off-Target effect “hepatotoxicity “frequently detected during clinical examination of patients on anti-Tb medication particularly isoniazid (INH), and rifampin (RMP). However, there is no any treatment option ag...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Metabolism Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589936823000270 |
| _version_ | 1797394795692294144 |
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| author | Endalkachew Gugsa Tewodros Shibabaw Molla Tesfahun Bekele Tadesse Asmamaw Dejenie |
| author_facet | Endalkachew Gugsa Tewodros Shibabaw Molla Tesfahun Bekele Tadesse Asmamaw Dejenie |
| author_sort | Endalkachew Gugsa |
| collection | DOAJ |
| description | Introduction: Drug-induced liver injury is the most common cause of acute liver failure. Off-Target effect “hepatotoxicity “frequently detected during clinical examination of patients on anti-Tb medication particularly isoniazid (INH), and rifampin (RMP). However, there is no any treatment option against isoniazid and rifampicin induced hepatotoxicity. It is, therefore, necessary to search for effective affordable and safe drugs from medicinal plants for the prevention of liver toxicity caused by isoniazid and rifampicin. The aim the current study is to evaluate hepatoprotective effect of hydro methanol extract from Otostegia integrifolia leaves in isoniazid and rifampicin-induced hepatotoxicity in Swiss albino mice. Methods: O. integrifolia leaves powder was macerated in hydromethanol and thirty Swiss albino mice 29.0–40.6 g were grouped in to five groups. Group I were given 20 ml/kg distilled water, group II were given 100 mg INH and 150 mg RIF per kg body weight. Group III, group IV, and group V were given 200 mg extract, 400 mg extract, and 100 mg of N-acetyl cysteine respectively per kg 1hr before induction with 100 mg INH plus 150 mg RIF per kg. The treatments were followed for 14 days. On the 15th day, all mice were anaesthetized with diethyl ether; blood samples were collected for the assessment liver enzyme and function test. Results: Group II mice's serum ALT, AST and total bilirubin levels were significantly increased and serum total protein and albumin levels were significantly decreased as compared with group I mice. The groups of mice treated with O. integrifolia at a dose of 400 mg/kg and N-acetyl cysteine AST, ALT and total bilirubin level were significantly decreased; and total protein and albumin levels were significantly (P < 0.05) increased as compared with group II. The liver index of the group IV showed decreased (P < 0.05) as compared to the group II. Conclusion: Evidence from our study revealed that the hydromethanol extract of O. integrifolia has a hepatoprotective effect against isoniazid and rifampicin-induced hepatotoxicity in Swiss Albino mice. This protective effect of O. integrifolia extract may be based on its metal ion reducing power, free radical scavenging activity, and anti-inflammatory activity and could be used as a potential therapeutic option. |
| first_indexed | 2024-03-09T00:24:54Z |
| format | Article |
| id | doaj.art-0e52656ee1974b019df2558055cb73fb |
| institution | Directory Open Access Journal |
| issn | 2589-9368 |
| language | English |
| last_indexed | 2024-03-09T00:24:54Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolism Open |
| spelling | doaj.art-0e52656ee1974b019df2558055cb73fb2023-12-12T04:35:23ZengElsevierMetabolism Open2589-93682023-12-0120100255Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino miceEndalkachew Gugsa0Tewodros Shibabaw Molla1Tesfahun Bekele2Tadesse Asmamaw Dejenie3Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, EthiopiaDepartment of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, EthiopiaDepartment of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, EthiopiaCorresponding author.; Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, EthiopiaIntroduction: Drug-induced liver injury is the most common cause of acute liver failure. Off-Target effect “hepatotoxicity “frequently detected during clinical examination of patients on anti-Tb medication particularly isoniazid (INH), and rifampin (RMP). However, there is no any treatment option against isoniazid and rifampicin induced hepatotoxicity. It is, therefore, necessary to search for effective affordable and safe drugs from medicinal plants for the prevention of liver toxicity caused by isoniazid and rifampicin. The aim the current study is to evaluate hepatoprotective effect of hydro methanol extract from Otostegia integrifolia leaves in isoniazid and rifampicin-induced hepatotoxicity in Swiss albino mice. Methods: O. integrifolia leaves powder was macerated in hydromethanol and thirty Swiss albino mice 29.0–40.6 g were grouped in to five groups. Group I were given 20 ml/kg distilled water, group II were given 100 mg INH and 150 mg RIF per kg body weight. Group III, group IV, and group V were given 200 mg extract, 400 mg extract, and 100 mg of N-acetyl cysteine respectively per kg 1hr before induction with 100 mg INH plus 150 mg RIF per kg. The treatments were followed for 14 days. On the 15th day, all mice were anaesthetized with diethyl ether; blood samples were collected for the assessment liver enzyme and function test. Results: Group II mice's serum ALT, AST and total bilirubin levels were significantly increased and serum total protein and albumin levels were significantly decreased as compared with group I mice. The groups of mice treated with O. integrifolia at a dose of 400 mg/kg and N-acetyl cysteine AST, ALT and total bilirubin level were significantly decreased; and total protein and albumin levels were significantly (P < 0.05) increased as compared with group II. The liver index of the group IV showed decreased (P < 0.05) as compared to the group II. Conclusion: Evidence from our study revealed that the hydromethanol extract of O. integrifolia has a hepatoprotective effect against isoniazid and rifampicin-induced hepatotoxicity in Swiss Albino mice. This protective effect of O. integrifolia extract may be based on its metal ion reducing power, free radical scavenging activity, and anti-inflammatory activity and could be used as a potential therapeutic option.http://www.sciencedirect.com/science/article/pii/S2589936823000270HepatotoxicityIsoniazidOtostegia integrifoliaOxidative stressRifampicin |
| spellingShingle | Endalkachew Gugsa Tewodros Shibabaw Molla Tesfahun Bekele Tadesse Asmamaw Dejenie Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice Metabolism Open Hepatotoxicity Isoniazid Otostegia integrifolia Oxidative stress Rifampicin |
| title | Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice |
| title_full | Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice |
| title_fullStr | Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice |
| title_full_unstemmed | Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice |
| title_short | Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice |
| title_sort | hepatoprotective effect of hydromethanol extract of otostegia integrifolia benth leaves in isoniazid and rifampicin induced swiss albino mice |
| topic | Hepatotoxicity Isoniazid Otostegia integrifolia Oxidative stress Rifampicin |
| url | http://www.sciencedirect.com/science/article/pii/S2589936823000270 |
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