The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis
Background The enhancement of immunity is very important for immunocompromised patients such as cancer patients with radiotherapy or chemotherapy. Glycyrrhiza uralensis has been used as food and medicine for a long history. G. uralensis polysaccharides (GUPS) were prepared and its immunostimulatory...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-01-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8294.pdf |
| _version_ | 1797420238902394880 |
|---|---|
| author | Adila Aipire Mahepali Mahabati Shanshan Cai Xianxian Wei Pengfei Yuan Alimu Aimaier Xinhui Wang Jinyao Li |
| author_facet | Adila Aipire Mahepali Mahabati Shanshan Cai Xianxian Wei Pengfei Yuan Alimu Aimaier Xinhui Wang Jinyao Li |
| author_sort | Adila Aipire |
| collection | DOAJ |
| description | Background The enhancement of immunity is very important for immunocompromised patients such as cancer patients with radiotherapy or chemotherapy. Glycyrrhiza uralensis has been used as food and medicine for a long history. G. uralensis polysaccharides (GUPS) were prepared and its immunostimulatory effects were investigated. Methods Human monocyte-derived dendritic cells (DCs) and murine bone marrow-derived DCs were treated with different concentrations of GUPS. The DCs maturation and cytokine production were analyzed by flow cytometry and ELISA, respectively. Inhibitors and Western blot were used to study the mechanism of GUPS. The immunostimulatory effects of GUPS were further evaluated by naïve mouse model and immunosuppressive mouse model induced by cyclophosphamide. Results GUPS significantly promoted the maturation and cytokine secretion of human monocyte-derived DCs and murine bone marrow-derived DCs through TLR4 and down-stream p38, JNK and NF-κB signaling pathways. Interestingly, the migration of GUPS treated-DCs to lymph node was increased. In the mouse model, GUPS increased IL-12 production in sera but not for TNF-α. Moreover, GUPS ameliorated the side effect of cyclophosphamide and improved the immunity of immunosuppressive mice induced by cyclophosphamide. These results suggested that GUPS might be used for cancer therapy to ameliorate the side effect of chemotherapy and enhance the immunity. |
| first_indexed | 2024-03-09T06:59:45Z |
| format | Article |
| id | doaj.art-0e53c5d3a979465e9d0e6cccdf7b83a5 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:59:45Z |
| publishDate | 2020-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-0e53c5d3a979465e9d0e6cccdf7b83a52023-12-03T09:55:37ZengPeerJ Inc.PeerJ2167-83592020-01-018e829410.7717/peerj.8294The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensisAdila Aipire0Mahepali Mahabati1Shanshan Cai2Xianxian Wei3Pengfei Yuan4Alimu Aimaier5Xinhui Wang6Jinyao Li7Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaCollege of Resource and Environment Sciences, Xinjiang University, Urumqi, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, ChinaBackground The enhancement of immunity is very important for immunocompromised patients such as cancer patients with radiotherapy or chemotherapy. Glycyrrhiza uralensis has been used as food and medicine for a long history. G. uralensis polysaccharides (GUPS) were prepared and its immunostimulatory effects were investigated. Methods Human monocyte-derived dendritic cells (DCs) and murine bone marrow-derived DCs were treated with different concentrations of GUPS. The DCs maturation and cytokine production were analyzed by flow cytometry and ELISA, respectively. Inhibitors and Western blot were used to study the mechanism of GUPS. The immunostimulatory effects of GUPS were further evaluated by naïve mouse model and immunosuppressive mouse model induced by cyclophosphamide. Results GUPS significantly promoted the maturation and cytokine secretion of human monocyte-derived DCs and murine bone marrow-derived DCs through TLR4 and down-stream p38, JNK and NF-κB signaling pathways. Interestingly, the migration of GUPS treated-DCs to lymph node was increased. In the mouse model, GUPS increased IL-12 production in sera but not for TNF-α. Moreover, GUPS ameliorated the side effect of cyclophosphamide and improved the immunity of immunosuppressive mice induced by cyclophosphamide. These results suggested that GUPS might be used for cancer therapy to ameliorate the side effect of chemotherapy and enhance the immunity.https://peerj.com/articles/8294.pdfGlycyrrhiza uralensis polysaccharidesDendritic cellMaturationCytokine productionMigrationSignaling pathway |
| spellingShingle | Adila Aipire Mahepali Mahabati Shanshan Cai Xianxian Wei Pengfei Yuan Alimu Aimaier Xinhui Wang Jinyao Li The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis PeerJ Glycyrrhiza uralensis polysaccharides Dendritic cell Maturation Cytokine production Migration Signaling pathway |
| title | The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis |
| title_full | The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis |
| title_fullStr | The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis |
| title_full_unstemmed | The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis |
| title_short | The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis |
| title_sort | immunostimulatory activity of polysaccharides from glycyrrhiza uralensis |
| topic | Glycyrrhiza uralensis polysaccharides Dendritic cell Maturation Cytokine production Migration Signaling pathway |
| url | https://peerj.com/articles/8294.pdf |
| work_keys_str_mv | AT adilaaipire theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT mahepalimahabati theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT shanshancai theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT xianxianwei theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT pengfeiyuan theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT alimuaimaier theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT xinhuiwang theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT jinyaoli theimmunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT adilaaipire immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT mahepalimahabati immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT shanshancai immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT xianxianwei immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT pengfeiyuan immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT alimuaimaier immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT xinhuiwang immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis AT jinyaoli immunostimulatoryactivityofpolysaccharidesfromglycyrrhizauralensis |