Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i>
Nitrogen (N) is an essential macronutrient for plant growth. Plants absorb and utilize N mainly in the form of nitrate (NO<sub>3</sub><sup>−</sup>) or ammonium (NH<sub>4</sub><sup>+</sup>). In this study, the nitrate transporter DsNRT3.1 (also known as...
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2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Hongping Ma Junchao Zhao Shuang Feng Kun Qiao Shufang Gong Jingang Wang Aimin Zhou |
| author_facet | Hongping Ma Junchao Zhao Shuang Feng Kun Qiao Shufang Gong Jingang Wang Aimin Zhou |
| author_sort | Hongping Ma |
| collection | DOAJ |
| description | Nitrogen (N) is an essential macronutrient for plant growth. Plants absorb and utilize N mainly in the form of nitrate (NO<sub>3</sub><sup>−</sup>) or ammonium (NH<sub>4</sub><sup>+</sup>). In this study, the nitrate transporter DsNRT3.1 (also known as the nitrate assimilation-related protein DsNAR2.1) was characterized from <i>Dianthus spiculifolius</i>. A quantitative PCR (qPCR) analysis showed that the <i>DsNRT3.1</i> expression was induced by NO<sub>3</sub><sup>−</sup>. Under N-starvation conditions, the transformed <i>Arabidopsis</i> seedlings expressing <i>DsNRT3.1</i> had longer roots and a greater fresh weight than the wild type. Subcellular localization showed that DsNRT3.1 was mainly localized to the plasma membrane in <i>Arabidopsis</i> root hair cells. Non-invasive micro-test (NMT) monitoring showed that the root hairs of N-starved transformed <i>Arabidopsis</i> seedlings had a stronger NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> influx than the wild-type seedlings, using with NO<sub>3</sub><sup>−</sup> or NH<sub>4</sub><sup>+</sup> as the sole N source; contrastingly, transformed seedlings only had a stronger NO<sub>3</sub><sup>−</sup> influx when NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> were present simultaneously. In addition, the qPCR analysis showed that the expression of <i>AtNRT2</i> genes (<i>AtNRT2.1</i><i>–2.6</i>), and particularly of <i>AtNRT2.5</i>, in the transformed <i>Arabidopsis</i> differed from that in the wild type. Overall, our results suggest that the heterologous expression of <i>DsNRT3.1</i> affects seedlings’ growth by enhancing the NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> uptake in N-starved <i>Arabidopsis</i>. This may be related to the differential expression of <i>AtNRT2</i> genes. |
| first_indexed | 2024-03-10T19:21:28Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:21:28Z |
| publishDate | 2020-06-01 |
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| spelling | doaj.art-0e58412c20c84192b6f46055ada9eeee2023-11-20T02:52:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012111402710.3390/ijms21114027Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i>Hongping Ma0Junchao Zhao1Shuang Feng2Kun Qiao3Shufang Gong4Jingang Wang5Aimin Zhou6College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaNitrogen (N) is an essential macronutrient for plant growth. Plants absorb and utilize N mainly in the form of nitrate (NO<sub>3</sub><sup>−</sup>) or ammonium (NH<sub>4</sub><sup>+</sup>). In this study, the nitrate transporter DsNRT3.1 (also known as the nitrate assimilation-related protein DsNAR2.1) was characterized from <i>Dianthus spiculifolius</i>. A quantitative PCR (qPCR) analysis showed that the <i>DsNRT3.1</i> expression was induced by NO<sub>3</sub><sup>−</sup>. Under N-starvation conditions, the transformed <i>Arabidopsis</i> seedlings expressing <i>DsNRT3.1</i> had longer roots and a greater fresh weight than the wild type. Subcellular localization showed that DsNRT3.1 was mainly localized to the plasma membrane in <i>Arabidopsis</i> root hair cells. Non-invasive micro-test (NMT) monitoring showed that the root hairs of N-starved transformed <i>Arabidopsis</i> seedlings had a stronger NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> influx than the wild-type seedlings, using with NO<sub>3</sub><sup>−</sup> or NH<sub>4</sub><sup>+</sup> as the sole N source; contrastingly, transformed seedlings only had a stronger NO<sub>3</sub><sup>−</sup> influx when NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> were present simultaneously. In addition, the qPCR analysis showed that the expression of <i>AtNRT2</i> genes (<i>AtNRT2.1</i><i>–2.6</i>), and particularly of <i>AtNRT2.5</i>, in the transformed <i>Arabidopsis</i> differed from that in the wild type. Overall, our results suggest that the heterologous expression of <i>DsNRT3.1</i> affects seedlings’ growth by enhancing the NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> uptake in N-starved <i>Arabidopsis</i>. This may be related to the differential expression of <i>AtNRT2</i> genes.https://www.mdpi.com/1422-0067/21/11/4027nitrogen<i>DsNRT3.1</i>heterologous expressionNO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> uptakeseedling growth<i>Arabidopsis</i> |
| spellingShingle | Hongping Ma Junchao Zhao Shuang Feng Kun Qiao Shufang Gong Jingang Wang Aimin Zhou Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i> International Journal of Molecular Sciences nitrogen <i>DsNRT3.1</i> heterologous expression NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> uptake seedling growth <i>Arabidopsis</i> |
| title | Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i> |
| title_full | Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i> |
| title_fullStr | Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i> |
| title_full_unstemmed | Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i> |
| title_short | Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved <i>Arabidopsis</i> |
| title_sort | heterologous expression of nitrate assimilation related protein dsnar2 1 nrt3 1 affects uptake of nitrate and ammonium in nitrogen starved i arabidopsis i |
| topic | nitrogen <i>DsNRT3.1</i> heterologous expression NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> uptake seedling growth <i>Arabidopsis</i> |
| url | https://www.mdpi.com/1422-0067/21/11/4027 |
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