PRODUCTION OF CERTAIN PRO- AND ANTI-INFLAMMATORY CYTOKINES DURING STIMULATION BY LIVE AND INACTIVATED LEPTOSPIRA PATHOGENS IN THE MODEL OF HUMAN WHOLE BLOOD

Aim. Study of the ability of clinical isolates of leptospira to cause production of certain pro-and anti-inflammatory cytokines in the model of human whole blood. Materials and methods. Leptospira interrogans strain was taken for the experiment. Cytokine content was determined by a method based on x...

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Bibliographic Details
Main Authors: O. A Petrova, N. A Stoyanova, N. K Tokarevich, N. A Arsentieva, N. E Lyubimova, A. V Semenov, A. Totolyan Areg
Format: Article
Language:Russian
Published: Central Research Institute for Epidemiology 2015-12-01
Series:Журнал микробиологии, эпидемиологии и иммунобиологии
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Online Access:https://microbiol.crie.ru/jour/article/view/14128
Description
Summary:Aim. Study of the ability of clinical isolates of leptospira to cause production of certain pro-and anti-inflammatory cytokines in the model of human whole blood. Materials and methods. Leptospira interrogans strain was taken for the experiment. Cytokine content was determined by a method based on xMAP technology using a standard panel, composed of 9 analytes: TNF-a, MСP-1, IL-8, IL-4, IL-6, IL-10, IL-1Rn, IL-12^70), IFN-y. Results. An optimal concentration of L. interrogans was selected for stimulation of human whole blood - 1x106 leptospirae/ml. For the first time in the model of human whole blood it was determined, that at early stages of incubation IFN-y, IL-12(p70), IL-4 and IL-1Ra are more actively produced; at later stages (6 hour incubation) - IL-8 and TNF-a. Conclusion. A differential pattern of cytokine production stimulation was shown in the model of human whole blood by live and inactivated leptospirae.
ISSN:0372-9311
2686-7613