Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study
Introduction: Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the most frequently encountered enzymopathy in humans. It is closely linked to neonatal jaundice, chronic non-spherocytic haemolytic anaemia, and acute haemolytic anaemia. G6PD deficiency is a common cause of jaundice among neonate...
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JCDR Research and Publications Pvt. Ltd.
2023-07-01
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| Series: | Indian Journal of Neonatal Medicine and Research |
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| Online Access: | https://www.ijnmr.net/articles/PDF/2391/62844_CE[Ra1]_F(SHU)_PF1(AKA_SHU)_PFA_(OM)_QC(SHK_SHU)_PN(SHU).pdf |
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| author | BHaskar Borah Sonowal Rita Panyang Kataki Alaka Das Arpita Gogoi |
| author_facet | BHaskar Borah Sonowal Rita Panyang Kataki Alaka Das Arpita Gogoi |
| author_sort | BHaskar Borah Sonowal |
| collection | DOAJ |
| description | Introduction: Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the most frequently encountered enzymopathy in humans. It is closely linked to neonatal jaundice, chronic non-spherocytic haemolytic anaemia, and acute haemolytic anaemia. G6PD deficiency is a common cause of jaundice among neonates.
Aim: To screen for G6PD deficiency among newborns at a tertiary care teaching centre in Assam.
Materials and Methods: This hospital-based cross-sectional study was conducted in the postnatal ward of the Department of Paediatrics at Medical College and Hospital, Dibrugarh, Assam, India, from June 2021 to May 2022. A randomly selected sample of 630 term neonates aged between 24 hours and 7 days of life was included in the study. A 2 mL blood sample was collected in an Ethylenediamine Tetra-acetic Acid (EDTA) vial from each neonate, and G6PD enzyme activity was estimated using the Kinetic Method with a commercially available G-six kit. Newborns with G6PD enzyme activity values less than 6.4 U/g Haemoglobin (Hb) were considered G6PD deficient. Data, including age, sex, religion, Total Serum Bilirubin (TSB/NBIL), G6PD activity, and Hb of the neonates, were entered into pre-designed forms. The data were analysed using the Chi-square test.
Results: Among the 630 screened neonates, 48 were found to be G6PD deficient, of which the majority were males (34 males, 14 females), resulting in a male-to-female ratio of 2.4:1. The occurrence of G6PD deficiency was 7.62%. The mean G6PD enzyme activity in deficient neonates was 4.53±1.17 U/g Hb.
Conclusion: This study identified a significant occurrence of G6PD deficiency in newborns, including females. The mean G6PD enzyme activity in deficient neonates was significantly lower than in normal cases. |
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| issn | 2277-8527 2455-6890 |
| language | English |
| last_indexed | 2024-04-24T07:22:45Z |
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| series | Indian Journal of Neonatal Medicine and Research |
| spelling | doaj.art-0e5b3af2b5a14419897691b76cb3b5e02024-04-21T01:54:25ZengJCDR Research and Publications Pvt. Ltd.Indian Journal of Neonatal Medicine and Research2277-85272455-68902023-07-01113PO05PO0810.7860/IJNMR/2023/62844.2391Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional StudyBHaskar Borah Sonowal0Rita Panyang Kataki1Alaka Das2Arpita Gogoi3Resident, Department of Paediatrics, Assam Medical College, Dibrugarh, Assam, India.Associate Professor, Department of Paediatrics, Assam Medical College, Dibrugarh, Assam, India.Associate Professor, Department of Biochemistry, Assam Medical College, Dibrugarh, Assam, India.Associate Professor, Department of Paediatrics, Assam Medical College, Dibrugarh, Assam, India.Introduction: Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the most frequently encountered enzymopathy in humans. It is closely linked to neonatal jaundice, chronic non-spherocytic haemolytic anaemia, and acute haemolytic anaemia. G6PD deficiency is a common cause of jaundice among neonates. Aim: To screen for G6PD deficiency among newborns at a tertiary care teaching centre in Assam. Materials and Methods: This hospital-based cross-sectional study was conducted in the postnatal ward of the Department of Paediatrics at Medical College and Hospital, Dibrugarh, Assam, India, from June 2021 to May 2022. A randomly selected sample of 630 term neonates aged between 24 hours and 7 days of life was included in the study. A 2 mL blood sample was collected in an Ethylenediamine Tetra-acetic Acid (EDTA) vial from each neonate, and G6PD enzyme activity was estimated using the Kinetic Method with a commercially available G-six kit. Newborns with G6PD enzyme activity values less than 6.4 U/g Haemoglobin (Hb) were considered G6PD deficient. Data, including age, sex, religion, Total Serum Bilirubin (TSB/NBIL), G6PD activity, and Hb of the neonates, were entered into pre-designed forms. The data were analysed using the Chi-square test. Results: Among the 630 screened neonates, 48 were found to be G6PD deficient, of which the majority were males (34 males, 14 females), resulting in a male-to-female ratio of 2.4:1. The occurrence of G6PD deficiency was 7.62%. The mean G6PD enzyme activity in deficient neonates was 4.53±1.17 U/g Hb. Conclusion: This study identified a significant occurrence of G6PD deficiency in newborns, including females. The mean G6PD enzyme activity in deficient neonates was significantly lower than in normal cases.https://www.ijnmr.net/articles/PDF/2391/62844_CE[Ra1]_F(SHU)_PF1(AKA_SHU)_PFA_(OM)_QC(SHK_SHU)_PN(SHU).pdfenzyme activityglucose-6-phosphate dehydrogenase deficiencyjaundiceneonate |
| spellingShingle | BHaskar Borah Sonowal Rita Panyang Kataki Alaka Das Arpita Gogoi Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study Indian Journal of Neonatal Medicine and Research enzyme activity glucose-6-phosphate dehydrogenase deficiency jaundice neonate |
| title | Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study |
| title_full | Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study |
| title_fullStr | Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study |
| title_full_unstemmed | Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study |
| title_short | Screening of G6PD Deficiency in Newborns at a Tertiary Care Teaching Hospital in Assam, North East India: A Cross-sectional Study |
| title_sort | screening of g6pd deficiency in newborns at a tertiary care teaching hospital in assam north east india a cross sectional study |
| topic | enzyme activity glucose-6-phosphate dehydrogenase deficiency jaundice neonate |
| url | https://www.ijnmr.net/articles/PDF/2391/62844_CE[Ra1]_F(SHU)_PF1(AKA_SHU)_PFA_(OM)_QC(SHK_SHU)_PN(SHU).pdf |
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