Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1)
<i>Fasciola gigantica</i>, a giant liver fluke, causes tremendous loss to the livestock economy in several regions throughout the world. The situation of drug resistance has been emerging increasingly; therefore, novel drugs and drug targets need to be discovered. The adult <i>F. g...
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1458 |
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| author | Pongsakorn Martviset Pathanin Chantree Salisa Chaimon Nattaya Torungkitmangmi Parisa Prathaphan Jittiporn Ruangtong Phornphan Sornchuer Nattaya Thongsepee Kant Sangpairoj Poom Adisakwattana |
| author_facet | Pongsakorn Martviset Pathanin Chantree Salisa Chaimon Nattaya Torungkitmangmi Parisa Prathaphan Jittiporn Ruangtong Phornphan Sornchuer Nattaya Thongsepee Kant Sangpairoj Poom Adisakwattana |
| author_sort | Pongsakorn Martviset |
| collection | DOAJ |
| description | <i>Fasciola gigantica</i>, a giant liver fluke, causes tremendous loss to the livestock economy in several regions throughout the world. The situation of drug resistance has been emerging increasingly; therefore, novel drugs and drug targets need to be discovered. The adult <i>F. gigantica</i> inhabits the major bile ducts where bile salts accumulate—these are steroid-like molecules that mediate several physiological processes in organisms through interacting with their specific nuclear receptors. However, the molecular mechanism of the interaction in the parasitic organisms have not been clearly understood. In this study, putative nuclear receptor subfamily 1 of <i>F. gigantica</i> (FgNR1) was identified. Nucleotide and amino acid sequences of the FgNR1 homolog were obtained from the transcriptome of <i>F. gigantica</i> and predicted for properties and functions using bioinformatics. The full-length cDNA was cloned and expressed in the bacterial expression system and then used for immunization. Western analysis and immunolocalization suggested that FgNR1 could be detected in the crude worm antigens and was highly expressed in the caeca and testes of the adult parasite. Moreover, the bile could significantly activate the expression of FgNR1 in cultured parasites. Our results indicated that FgNR1 has high potential for the development of a novel anthelminthic drug in the future. |
| first_indexed | 2024-03-09T15:59:00Z |
| format | Article |
| id | doaj.art-0e5c18d81f614c3993213102bb9e41a1 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:00Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-0e5c18d81f614c3993213102bb9e41a12023-11-24T17:13:04ZengMDPI AGPathogens2076-08172022-12-011112145810.3390/pathogens11121458Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1)Pongsakorn Martviset0Pathanin Chantree1Salisa Chaimon2Nattaya Torungkitmangmi3Parisa Prathaphan4Jittiporn Ruangtong5Phornphan Sornchuer6Nattaya Thongsepee7Kant Sangpairoj8Poom Adisakwattana9Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandThammasat University Research Unit in Nutraceuticals and Food Safety, Thammasat University, Pathumthani 12120, ThailandGraduate Program in Biochemistry and Molecular Biology, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandThammasat University Research Unit in Nutraceuticals and Food Safety, Thammasat University, Pathumthani 12120, ThailandThammasat University Research Unit in Nutraceuticals and Food Safety, Thammasat University, Pathumthani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand<i>Fasciola gigantica</i>, a giant liver fluke, causes tremendous loss to the livestock economy in several regions throughout the world. The situation of drug resistance has been emerging increasingly; therefore, novel drugs and drug targets need to be discovered. The adult <i>F. gigantica</i> inhabits the major bile ducts where bile salts accumulate—these are steroid-like molecules that mediate several physiological processes in organisms through interacting with their specific nuclear receptors. However, the molecular mechanism of the interaction in the parasitic organisms have not been clearly understood. In this study, putative nuclear receptor subfamily 1 of <i>F. gigantica</i> (FgNR1) was identified. Nucleotide and amino acid sequences of the FgNR1 homolog were obtained from the transcriptome of <i>F. gigantica</i> and predicted for properties and functions using bioinformatics. The full-length cDNA was cloned and expressed in the bacterial expression system and then used for immunization. Western analysis and immunolocalization suggested that FgNR1 could be detected in the crude worm antigens and was highly expressed in the caeca and testes of the adult parasite. Moreover, the bile could significantly activate the expression of FgNR1 in cultured parasites. Our results indicated that FgNR1 has high potential for the development of a novel anthelminthic drug in the future.https://www.mdpi.com/2076-0817/11/12/1458<i>Fasciola gigantica</i>FgNR1nuclear receptordrug target |
| spellingShingle | Pongsakorn Martviset Pathanin Chantree Salisa Chaimon Nattaya Torungkitmangmi Parisa Prathaphan Jittiporn Ruangtong Phornphan Sornchuer Nattaya Thongsepee Kant Sangpairoj Poom Adisakwattana Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1) Pathogens <i>Fasciola gigantica</i> FgNR1 nuclear receptor drug target |
| title | Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1) |
| title_full | Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1) |
| title_fullStr | Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1) |
| title_full_unstemmed | Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1) |
| title_short | Molecular Cloning and Characterization of a <i>Fasciola gigantica</i> Nuclear Receptor Subfamily 1 (FgNR1) |
| title_sort | molecular cloning and characterization of a i fasciola gigantica i nuclear receptor subfamily 1 fgnr1 |
| topic | <i>Fasciola gigantica</i> FgNR1 nuclear receptor drug target |
| url | https://www.mdpi.com/2076-0817/11/12/1458 |
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