Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report
Leptospirosis is a zoonotic infection for which diagnosis is difficult. It has appeared as a global emerging infectious disease over recent years. Genotype determination often requires a Leptospira strain obtained by culture, which is a long and fastidious technique. A method based on multilocus var...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-08-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971215001642 |
| _version_ | 1819059369621323776 |
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| author | Hélène Pailhoriès Rodolphe Buzelé Mathieu Picardeau Sylvie Robert Emmanuelle Mercier Laurent Mereghetti Philippe Lanotte |
| author_facet | Hélène Pailhoriès Rodolphe Buzelé Mathieu Picardeau Sylvie Robert Emmanuelle Mercier Laurent Mereghetti Philippe Lanotte |
| author_sort | Hélène Pailhoriès |
| collection | DOAJ |
| description | Leptospirosis is a zoonotic infection for which diagnosis is difficult. It has appeared as a global emerging infectious disease over recent years. Genotype determination often requires a Leptospira strain obtained by culture, which is a long and fastidious technique. A method based on multilocus variable number tandem repeat analysis (MLVA) to determine the genotype of Leptospira interrogans, performed directly on blood or urine samples, is proposed. This method was applied to a fatal case of leptospirosis for which the geographical origin of infection was unknown. This technique will allow a genotype to be obtained for L. interrogans, even when cultures remain negative. |
| first_indexed | 2024-12-21T14:10:00Z |
| format | Article |
| id | doaj.art-0e5d990d0cd4429488f421061d811d6d |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-21T14:10:00Z |
| publishDate | 2015-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-0e5d990d0cd4429488f421061d811d6d2022-12-21T19:01:04ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112015-08-0137C11912110.1016/j.ijid.2015.06.026Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case reportHélène Pailhoriès0Rodolphe Buzelé1Mathieu Picardeau2Sylvie Robert3Emmanuelle Mercier4Laurent Mereghetti5Philippe Lanotte6CHU d’Angers, Laboratoire de Bactériologie, Institut de Biologie en Santé, Angers, FranceService de Bactériologie-Virologie, Hôpital Bretonneau – CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, FranceInstitut Pasteur, Unité de Biologie des Spirochètes, National Reference Centre and WHO Collaborating Centre for Leptospirosis, Paris, FranceService de Bactériologie-Virologie, Hôpital Bretonneau – CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, FranceCHRU de Tours, Service de Réanimation Médicale, Tours, FranceService de Bactériologie-Virologie, Hôpital Bretonneau – CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, FranceService de Bactériologie-Virologie, Hôpital Bretonneau – CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, FranceLeptospirosis is a zoonotic infection for which diagnosis is difficult. It has appeared as a global emerging infectious disease over recent years. Genotype determination often requires a Leptospira strain obtained by culture, which is a long and fastidious technique. A method based on multilocus variable number tandem repeat analysis (MLVA) to determine the genotype of Leptospira interrogans, performed directly on blood or urine samples, is proposed. This method was applied to a fatal case of leptospirosis for which the geographical origin of infection was unknown. This technique will allow a genotype to be obtained for L. interrogans, even when cultures remain negative.http://www.sciencedirect.com/science/article/pii/S1201971215001642LeptospirosisGenotypeVNTRMLVA |
| spellingShingle | Hélène Pailhoriès Rodolphe Buzelé Mathieu Picardeau Sylvie Robert Emmanuelle Mercier Laurent Mereghetti Philippe Lanotte Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report International Journal of Infectious Diseases Leptospirosis Genotype VNTR MLVA |
| title | Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report |
| title_full | Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report |
| title_fullStr | Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report |
| title_full_unstemmed | Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report |
| title_short | Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report |
| title_sort | molecular characterization of leptospira sp by multilocus variable number tandem repeat analysis mlva from clinical samples a case report |
| topic | Leptospirosis Genotype VNTR MLVA |
| url | http://www.sciencedirect.com/science/article/pii/S1201971215001642 |
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