| Summary: | This study was conducted to investigate the effect of cell wall yeast, aluminosilicate and some probiotic strains in diets contaminated with
aflatoxin, on the expression of GOT2, CYP450 1A5 genes and serum concentrations of liver enzymes including GGT, ALT, AST and LDH in broilers.
400 seven-days old chicks from strain Cobb 500 were used as a completely randomized design with 8 treatments, 5 replications. Treatments
were included: 1- Negative control (NC), 2- NC + 1 mg/kg aflatoxin or positive control (PC), 3- PC + 1 kg/ton Toxeat® (a toxin binder), 4- PC +
Lactobacillus strains (L), 5- PC + Bacillus subtilis JQ618 strain (B), 6- PC + Saccharomyces cerevisiaeʼs cell wall (Y), 7- PC + [PC+ BLY ) B+ L+ Y(], 8- PC +
Hydrated sodium calcium Aluminosilicate (HA). The serum concentrations of LDH, ALT, GGT and AST were increased in PC group at 42d (P˂0.05).
Tox®, L and BLY reduced serum levels of AST (P˂0.05). Increased serum concentration of GGT was observed in PC treatment, decreased by HA, Y,
B and L treatments (P˂0.05). The results showed the upregulation of GOT2, CYP450 1A5 in PC group. But Y, B, L and Tox® reduced the expression of
GOT2. The groups receiving aflatoxin adsorbent compounds reduced the adverse effects of aflatoxin on increasing the expression of CYP450 1A5.
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