Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study
Abstract Background Betel-nut consumption is the fourth most common addictive habit globally and there is good evidence linking the habit to obesity, type 2 diabetes (T2D) and the metabolic syndrome. The aim of our pilot study was to identify gene expression relevant to obesity, T2D and the metaboli...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | BMC Endocrine Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12902-021-00827-1 |
| _version_ | 1819094697713336320 |
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| author | Shirleny R Cardosa B. William Ogunkolade Rob Lowe Emanuel Savage Charles A Mein Barbara J Boucher Graham A Hitman |
| author_facet | Shirleny R Cardosa B. William Ogunkolade Rob Lowe Emanuel Savage Charles A Mein Barbara J Boucher Graham A Hitman |
| author_sort | Shirleny R Cardosa |
| collection | DOAJ |
| description | Abstract Background Betel-nut consumption is the fourth most common addictive habit globally and there is good evidence linking the habit to obesity, type 2 diabetes (T2D) and the metabolic syndrome. The aim of our pilot study was to identify gene expression relevant to obesity, T2D and the metabolic syndrome using a genome-wide transcriptomic approach in a human monocyte cell line incubated with arecoline and its nitrosated products. Results The THP1 monocyte cell line was incubated separately with arecoline and 3-methylnitrosaminopropionaldehyde (MNPA) in triplicate for 24 h and pooled cDNA indexed paired-end libraries were sequenced (Illumina NextSeq 500). After incubation with arecoline and MNPA, 15 and 39 genes respectively had significant changes in their expression (q < 0.05, log fold change 1.5). Eighteen of those genes have reported associations with T2D and obesity in humans; of these genes there was most marked evidence for CLEC10A, MAPK8IP1, NEGR1, NQ01 and INHBE genes. Conclusions Our preliminary studies have identified a large number of genes relevant to obesity, T2D and metabolic syndrome whose expression was changed significantly in human TPH1 cells following incubation with betel-nut derived arecoline or with MNPA. These findings require validation by further cell-based work and investigation amongst betel-chewing communities. |
| first_indexed | 2024-12-21T23:31:32Z |
| format | Article |
| id | doaj.art-0e6031ae7c5145e1967a4d3e913bcf7d |
| institution | Directory Open Access Journal |
| issn | 1472-6823 |
| language | English |
| last_indexed | 2024-12-21T23:31:32Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj.art-0e6031ae7c5145e1967a4d3e913bcf7d2022-12-21T18:46:28ZengBMCBMC Endocrine Disorders1472-68232021-08-0121111110.1186/s12902-021-00827-1Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot studyShirleny R Cardosa0B. William Ogunkolade1Rob Lowe2Emanuel Savage3Charles A Mein4Barbara J Boucher5Graham A Hitman6Centre for Genomics and Child Health, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of LondonCentre for Genomics and Child Health, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of LondonCentre for Genomics and Child Health, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of LondonBarts and The London Genome Centre, Blizard Institute, Queen Mary University of LondonBarts and The London Genome Centre, Blizard Institute, Queen Mary University of LondonCentre for Genomics and Child Health, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of LondonCentre for Genomics and Child Health, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of LondonAbstract Background Betel-nut consumption is the fourth most common addictive habit globally and there is good evidence linking the habit to obesity, type 2 diabetes (T2D) and the metabolic syndrome. The aim of our pilot study was to identify gene expression relevant to obesity, T2D and the metabolic syndrome using a genome-wide transcriptomic approach in a human monocyte cell line incubated with arecoline and its nitrosated products. Results The THP1 monocyte cell line was incubated separately with arecoline and 3-methylnitrosaminopropionaldehyde (MNPA) in triplicate for 24 h and pooled cDNA indexed paired-end libraries were sequenced (Illumina NextSeq 500). After incubation with arecoline and MNPA, 15 and 39 genes respectively had significant changes in their expression (q < 0.05, log fold change 1.5). Eighteen of those genes have reported associations with T2D and obesity in humans; of these genes there was most marked evidence for CLEC10A, MAPK8IP1, NEGR1, NQ01 and INHBE genes. Conclusions Our preliminary studies have identified a large number of genes relevant to obesity, T2D and metabolic syndrome whose expression was changed significantly in human TPH1 cells following incubation with betel-nut derived arecoline or with MNPA. These findings require validation by further cell-based work and investigation amongst betel-chewing communities.https://doi.org/10.1186/s12902-021-00827-1Betel-nutType 2 diabetesObesityTranscriptomicsRNA-sequencing |
| spellingShingle | Shirleny R Cardosa B. William Ogunkolade Rob Lowe Emanuel Savage Charles A Mein Barbara J Boucher Graham A Hitman Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study BMC Endocrine Disorders Betel-nut Type 2 diabetes Obesity Transcriptomics RNA-sequencing |
| title | Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study |
| title_full | Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study |
| title_fullStr | Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study |
| title_full_unstemmed | Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study |
| title_short | Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study |
| title_sort | areca catechu betel nut induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity a pilot study |
| topic | Betel-nut Type 2 diabetes Obesity Transcriptomics RNA-sequencing |
| url | https://doi.org/10.1186/s12902-021-00827-1 |
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