Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice
Alzheimer’s disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and the accumulation of hyperphosphorylated tau, both of which are accompanied by neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in...
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MDPI AG
2021-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/7/2369 |
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| author | Cristina Rosell-Cardona Christian Griñan-Ferré Anna Pérez-Bosque Javier Polo Mercè Pallàs Concepció Amat Miquel Moretó Lluïsa Miró |
| author_facet | Cristina Rosell-Cardona Christian Griñan-Ferré Anna Pérez-Bosque Javier Polo Mercè Pallàs Concepció Amat Miquel Moretó Lluïsa Miró |
| author_sort | Cristina Rosell-Cardona |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and the accumulation of hyperphosphorylated tau, both of which are accompanied by neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in inflammation models. We investigated whether dietary supplementation with SDP prevents the neuropathological features of AD. The experiments were performed in 2- and 6-month-old SAMP8 mice fed a control diet, or a diet supplemented with 8% SDP, for 4 months. AD brain molecular markers were determined by Western blot and real-time PCR. Senescent mice showed reduced levels of p-GSK3β (Ser9) and an increase in p-CDK5, p-tau (Ser396), sAPPβ, and the concentration of Aβ<sub>40,</sub> (all <i>p</i> < 0.05). SDP prevented these effects of aging and reduced <i>Bace1</i> levels (all <i>p</i> < 0.05). Senescence increased the expression of <i>Mme1</i> and <i>Ide1</i> and pro-inflammatory cytokines (<i>Il-17</i> and <i>Il-18</i>; all <i>p</i> < 0.05); these changes were prevented by SDP supplementation. Moreover, SDP increased <i>Tgf-β</i> expression (<i>p</i> < 0.05). Furthermore, in aged mice, the gene expression levels of the microglial activation markers <i>Trem2</i>, <i>Ym1</i>, and <i>Arg1</i> were increased, and SDP prevented these increases (all <i>p</i> < 0.05). Thus, dietary SDP might delay AD onset by reducing its hallmarks in senescent mice. |
| first_indexed | 2024-03-10T09:29:11Z |
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| id | doaj.art-0e61cc97701d4ef6b60ca5d56dc92346 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T09:29:11Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-0e61cc97701d4ef6b60ca5d56dc923462023-11-22T04:36:26ZengMDPI AGNutrients2072-66432021-07-01137236910.3390/nu13072369Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 MiceCristina Rosell-Cardona0Christian Griñan-Ferré1Anna Pérez-Bosque2Javier Polo3Mercè Pallàs4Concepció Amat5Miquel Moretó6Lluïsa Miró7Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Pharmacology, Toxicology, and Medicinal Chemistry, Faculty of Pharmacy and Food Sciences, Institute of Neurosciences, CIBERNED, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainAPC Europe S.L.U., 08403 Granollers, SpainDepartment of Pharmacology, Toxicology, and Medicinal Chemistry, Faculty of Pharmacy and Food Sciences, Institute of Neurosciences, CIBERNED, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainAlzheimer’s disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and the accumulation of hyperphosphorylated tau, both of which are accompanied by neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in inflammation models. We investigated whether dietary supplementation with SDP prevents the neuropathological features of AD. The experiments were performed in 2- and 6-month-old SAMP8 mice fed a control diet, or a diet supplemented with 8% SDP, for 4 months. AD brain molecular markers were determined by Western blot and real-time PCR. Senescent mice showed reduced levels of p-GSK3β (Ser9) and an increase in p-CDK5, p-tau (Ser396), sAPPβ, and the concentration of Aβ<sub>40,</sub> (all <i>p</i> < 0.05). SDP prevented these effects of aging and reduced <i>Bace1</i> levels (all <i>p</i> < 0.05). Senescence increased the expression of <i>Mme1</i> and <i>Ide1</i> and pro-inflammatory cytokines (<i>Il-17</i> and <i>Il-18</i>; all <i>p</i> < 0.05); these changes were prevented by SDP supplementation. Moreover, SDP increased <i>Tgf-β</i> expression (<i>p</i> < 0.05). Furthermore, in aged mice, the gene expression levels of the microglial activation markers <i>Trem2</i>, <i>Ym1</i>, and <i>Arg1</i> were increased, and SDP prevented these increases (all <i>p</i> < 0.05). Thus, dietary SDP might delay AD onset by reducing its hallmarks in senescent mice.https://www.mdpi.com/2072-6643/13/7/2369neuroinflammationdietary supplementationagingplasma proteinsAlzheimer’s diseaseSAMP8 |
| spellingShingle | Cristina Rosell-Cardona Christian Griñan-Ferré Anna Pérez-Bosque Javier Polo Mercè Pallàs Concepció Amat Miquel Moretó Lluïsa Miró Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice Nutrients neuroinflammation dietary supplementation aging plasma proteins Alzheimer’s disease SAMP8 |
| title | Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice |
| title_full | Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice |
| title_fullStr | Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice |
| title_full_unstemmed | Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice |
| title_short | Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice |
| title_sort | dietary spray dried porcine plasma reduces neuropathological alzheimer s disease hallmarks in samp8 mice |
| topic | neuroinflammation dietary supplementation aging plasma proteins Alzheimer’s disease SAMP8 |
| url | https://www.mdpi.com/2072-6643/13/7/2369 |
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