<b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil
Tibouchina hatschbachii Wurdack (Melastomataceae) is an autogamous shrub restricted to granite (GO) and sandstone (SO) rock outcrops from subtropical Brazil. We designed primers for the amplification of microsatellite regions for T. hatschbachii, and characterized these primers to estimate genetic d...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Maringá
2016-12-01
|
| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31496 |
| _version_ | 1828464762513522688 |
|---|---|
| author | Fabiano Rodrigo Maia Patricia Sanae Sujii Renato Goldenberg Viviane Silva Pereira Maria Imaculada Zucchi |
| author_facet | Fabiano Rodrigo Maia Patricia Sanae Sujii Renato Goldenberg Viviane Silva Pereira Maria Imaculada Zucchi |
| author_sort | Fabiano Rodrigo Maia |
| collection | DOAJ |
| description | Tibouchina hatschbachii Wurdack (Melastomataceae) is an autogamous shrub restricted to granite (GO) and sandstone (SO) rock outcrops from subtropical Brazil. We designed primers for the amplification of microsatellite regions for T. hatschbachii, and characterized these primers to estimate genetic diversity parameters and contemporary genetic structure patterns. Eight loci were successfully amplified and were characterized using 70 individuals from three natural populations. Polymorphic information content ranged from 0.200 to 0.772 per locus. All loci were polymorphic, with allele numbers ranging from two to eight. The low degree of polymorphism may be explained by the fact that T. hatschbachii has disjunct populations and a recent genetic bottleneck, and also that it is self-pollinated. The observed and expected heterozygosities ranged from 0.115 to 1.000 and from 0.112 to 0.800, respectively. We observed private alleles in all loci. These are important features that enable us to identify population differentiation and help to us understand gene flow patterns for T. hatschbachii in subtropical Brazil. Eight microsatellite loci from other species of Tibouchina amplified positively in T. hatschbachii.
|
| first_indexed | 2024-12-11T03:20:07Z |
| format | Article |
| id | doaj.art-0e642cbc40404532a8c73880ceb202a8 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-11T03:20:07Z |
| publishDate | 2016-12-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-0e642cbc40404532a8c73880ceb202a82022-12-22T01:22:39ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2016-12-0138310.4025/actascibiolsci.v38i3.3149614296<b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from BrazilFabiano Rodrigo Maia0Patricia Sanae Sujii1Renato Goldenberg2Viviane Silva Pereira3Maria Imaculada Zucchi4Universidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Federal do ParanáUniversidade Federal do ParanáAgência Paulista de Tecnologia dos AgronegóciosTibouchina hatschbachii Wurdack (Melastomataceae) is an autogamous shrub restricted to granite (GO) and sandstone (SO) rock outcrops from subtropical Brazil. We designed primers for the amplification of microsatellite regions for T. hatschbachii, and characterized these primers to estimate genetic diversity parameters and contemporary genetic structure patterns. Eight loci were successfully amplified and were characterized using 70 individuals from three natural populations. Polymorphic information content ranged from 0.200 to 0.772 per locus. All loci were polymorphic, with allele numbers ranging from two to eight. The low degree of polymorphism may be explained by the fact that T. hatschbachii has disjunct populations and a recent genetic bottleneck, and also that it is self-pollinated. The observed and expected heterozygosities ranged from 0.115 to 1.000 and from 0.112 to 0.800, respectively. We observed private alleles in all loci. These are important features that enable us to identify population differentiation and help to us understand gene flow patterns for T. hatschbachii in subtropical Brazil. Eight microsatellite loci from other species of Tibouchina amplified positively in T. hatschbachii. https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31496MelastomataceaeTibouchinagranitesandstonerock outcropspopulation genetics |
| spellingShingle | Fabiano Rodrigo Maia Patricia Sanae Sujii Renato Goldenberg Viviane Silva Pereira Maria Imaculada Zucchi <b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil Acta Scientiarum: Biological Sciences Melastomataceae Tibouchina granite sandstone rock outcrops population genetics |
| title | <b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil |
| title_full | <b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil |
| title_fullStr | <b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil |
| title_full_unstemmed | <b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil |
| title_short | <b>Development and characterization of microsatellite markers for <i>Tibouchina hatschbachii</i> (Melastomataceae), an endemic and habitat-restricted shrub from Brazil |
| title_sort | b development and characterization of microsatellite markers for i tibouchina hatschbachii i melastomataceae an endemic and habitat restricted shrub from brazil |
| topic | Melastomataceae Tibouchina granite sandstone rock outcrops population genetics |
| url | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31496 |
| work_keys_str_mv | AT fabianorodrigomaia bdevelopmentandcharacterizationofmicrosatellitemarkersforitibouchinahatschbachiiimelastomataceaeanendemicandhabitatrestrictedshrubfrombrazil AT patriciasanaesujii bdevelopmentandcharacterizationofmicrosatellitemarkersforitibouchinahatschbachiiimelastomataceaeanendemicandhabitatrestrictedshrubfrombrazil AT renatogoldenberg bdevelopmentandcharacterizationofmicrosatellitemarkersforitibouchinahatschbachiiimelastomataceaeanendemicandhabitatrestrictedshrubfrombrazil AT vivianesilvapereira bdevelopmentandcharacterizationofmicrosatellitemarkersforitibouchinahatschbachiiimelastomataceaeanendemicandhabitatrestrictedshrubfrombrazil AT mariaimaculadazucchi bdevelopmentandcharacterizationofmicrosatellitemarkersforitibouchinahatschbachiiimelastomataceaeanendemicandhabitatrestrictedshrubfrombrazil |