Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells
Abstract Hyperthermia is one of the most patient-friendly methods to cure cancer diseases owing to its noninvasiveness, minimally induced side-effects and toxicity, and easy implementation, prompting the development of novel therapeutic methods like photothermally triggering dose system. This resear...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-06-01
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| Series: | Nanoscale Research Letters |
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| Online Access: | https://doi.org/10.1186/s11671-021-03565-4 |
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| author | Po-Sheng Hu Hsiu-Jen Chou Chi-An Chen Po-Yi Wu Kai-Hsien Hsiao Yu-Min Kuo |
| author_facet | Po-Sheng Hu Hsiu-Jen Chou Chi-An Chen Po-Yi Wu Kai-Hsien Hsiao Yu-Min Kuo |
| author_sort | Po-Sheng Hu |
| collection | DOAJ |
| description | Abstract Hyperthermia is one of the most patient-friendly methods to cure cancer diseases owing to its noninvasiveness, minimally induced side-effects and toxicity, and easy implementation, prompting the development of novel therapeutic methods like photothermally triggering dose system. This research herein interrogates the variables of photothermal effects of Cs0.33WO3 nanoparticles (NPs), the duration of irradiation, optical power density and NP concentration, upon HepG2 liver cancer cell line in vitro, leading to the formulation of a near-infrared (NIR)-irradiated thermal dose. Expressly, the NPs with particulate feature sizes of 120 nm were synthesized through a series of oxidation–reduction (REDOX) reaction, thermal annealing and wet-grinding processes, and the subsequent characterization of physical, compositional, optical, photothermal properties were examined using dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDS), scanning and tunneling electron microscopies (SEM and TEM), X-ray diffraction (XRD) and visible-near-infrared (VIS–NIR) photospectroscopy. Cytotoxicity of the NPs and its irradiation parameters were obtained for the HepG2 cells. By incubating the cells with the NPs, the state of endocytosis was verified, and the dependence of cellular survival rate on the variable parameters of photothermal dose was determined while maintaining the medium temperature of the cell-containing culture dish at human body temperature around 36.5 °C. |
| first_indexed | 2024-03-12T08:54:28Z |
| format | Article |
| id | doaj.art-0e6ae66d19994959be58c7de7428bd01 |
| institution | Directory Open Access Journal |
| issn | 1556-276X |
| language | English |
| last_indexed | 2024-03-12T08:54:28Z |
| publishDate | 2021-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-0e6ae66d19994959be58c7de7428bd012023-09-02T16:08:29ZengSpringerOpenNanoscale Research Letters1556-276X2021-06-0116111010.1186/s11671-021-03565-4Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer CellsPo-Sheng Hu0Hsiu-Jen Chou1Chi-An Chen2Po-Yi Wu3Kai-Hsien Hsiao4Yu-Min Kuo5College of Photonics, National Yang Ming Chiao Tung UniversityCollege of Photonics, National Yang Ming Chiao Tung UniversityCollege of Photonics, National Yang Ming Chiao Tung UniversityCollege of Photonics, National Yang Ming Chiao Tung UniversityCollege of Photonics, National Yang Ming Chiao Tung UniversityDepartment of Cell Biology and Anatomy, College of Medicine, National Cheng Kung UniversityAbstract Hyperthermia is one of the most patient-friendly methods to cure cancer diseases owing to its noninvasiveness, minimally induced side-effects and toxicity, and easy implementation, prompting the development of novel therapeutic methods like photothermally triggering dose system. This research herein interrogates the variables of photothermal effects of Cs0.33WO3 nanoparticles (NPs), the duration of irradiation, optical power density and NP concentration, upon HepG2 liver cancer cell line in vitro, leading to the formulation of a near-infrared (NIR)-irradiated thermal dose. Expressly, the NPs with particulate feature sizes of 120 nm were synthesized through a series of oxidation–reduction (REDOX) reaction, thermal annealing and wet-grinding processes, and the subsequent characterization of physical, compositional, optical, photothermal properties were examined using dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDS), scanning and tunneling electron microscopies (SEM and TEM), X-ray diffraction (XRD) and visible-near-infrared (VIS–NIR) photospectroscopy. Cytotoxicity of the NPs and its irradiation parameters were obtained for the HepG2 cells. By incubating the cells with the NPs, the state of endocytosis was verified, and the dependence of cellular survival rate on the variable parameters of photothermal dose was determined while maintaining the medium temperature of the cell-containing culture dish at human body temperature around 36.5 °C.https://doi.org/10.1186/s11671-021-03565-4HepG2 cellCesium tungsten oxide nanoparticlesHyperthermia dosePhotothermal triggerCytotoxicity |
| spellingShingle | Po-Sheng Hu Hsiu-Jen Chou Chi-An Chen Po-Yi Wu Kai-Hsien Hsiao Yu-Min Kuo Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells Nanoscale Research Letters HepG2 cell Cesium tungsten oxide nanoparticles Hyperthermia dose Photothermal trigger Cytotoxicity |
| title | Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells |
| title_full | Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells |
| title_fullStr | Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells |
| title_full_unstemmed | Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells |
| title_short | Devising Hyperthermia Dose of NIR-Irradiated Cs0.33WO3 Nanoparticles for HepG2 Hepatic Cancer Cells |
| title_sort | devising hyperthermia dose of nir irradiated cs0 33wo3 nanoparticles for hepg2 hepatic cancer cells |
| topic | HepG2 cell Cesium tungsten oxide nanoparticles Hyperthermia dose Photothermal trigger Cytotoxicity |
| url | https://doi.org/10.1186/s11671-021-03565-4 |
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