Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings
Abstract Pseudogymnoascus destructans (Pd) is the causative agent of white-nose syndrome, which has resulted in the death of millions of bats in North America (NA) since 2006. Based on mortalities in eastern NA, the westward spread of infections likely poses a significant threat to western NA bats....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14223-9 |
| _version_ | 1818236803644129280 |
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| author | Adrian Forsythe Nick Fontaine Julianna Bissonnette Brandon Hayashi Chadabhorn Insuk Soumya Ghosh Gabrielle Kam Aaron Wong Cori Lausen Jianping Xu Naowarat Cheeptham |
| author_facet | Adrian Forsythe Nick Fontaine Julianna Bissonnette Brandon Hayashi Chadabhorn Insuk Soumya Ghosh Gabrielle Kam Aaron Wong Cori Lausen Jianping Xu Naowarat Cheeptham |
| author_sort | Adrian Forsythe |
| collection | DOAJ |
| description | Abstract Pseudogymnoascus destructans (Pd) is the causative agent of white-nose syndrome, which has resulted in the death of millions of bats in North America (NA) since 2006. Based on mortalities in eastern NA, the westward spread of infections likely poses a significant threat to western NA bats. To help prevent/reduce Pd infections in bats in western NA, we isolated bacteria from the wings of wild bats and screened for inhibitory activity against Pd. In total, we obtained 1,362 bacterial isolates from 265 wild bats of 13 species in western Canada. Among the 1,362 isolates, 96 showed inhibitory activity against Pd based on a coculture assay. The inhibitory activities varied widely among these isolates, ranging from slowing fungal growth to complete inhibition. Interestingly, host bats containing isolates with anti-Pd activities were widely distributed, with no apparent geographic or species-specific pattern. However, characteristics of roosting sites and host demography showed significant associations with the isolation of anti-Pd bacteria. Specifically, anthropogenic roosts and swabs from young males had higher frequencies of anti-Pd bacteria than those from natural roosts and those from other sex and age-groups, respectively. These anti-Pd bacteria could be potentially used to help mitigate the impact of WNS. Field trials using these as well as additional microbes from future screenings are needed in order to determine their effectiveness for the prevention and treatment against WNS. |
| first_indexed | 2024-12-12T12:15:40Z |
| format | Article |
| id | doaj.art-0e6aec8e1d3d48e49aca5b58ce513181 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T12:15:40Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0e6aec8e1d3d48e49aca5b58ce5131812022-12-22T00:24:46ZengNature PortfolioScientific Reports2045-23222022-06-0112111610.1038/s41598-022-14223-9Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wingsAdrian Forsythe0Nick Fontaine1Julianna Bissonnette2Brandon Hayashi3Chadabhorn Insuk4Soumya Ghosh5Gabrielle Kam6Aaron Wong7Cori Lausen8Jianping Xu9Naowarat Cheeptham10Department of Biology, Faculty of Science, McMaster UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityDepartment of Biology, Faculty of Science, McMaster UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityWildlife Conservation Society CanadaDepartment of Biology, Faculty of Science, McMaster UniversityDepartment of Biological Sciences, Faculty of Science, Thompson Rivers UniversityAbstract Pseudogymnoascus destructans (Pd) is the causative agent of white-nose syndrome, which has resulted in the death of millions of bats in North America (NA) since 2006. Based on mortalities in eastern NA, the westward spread of infections likely poses a significant threat to western NA bats. To help prevent/reduce Pd infections in bats in western NA, we isolated bacteria from the wings of wild bats and screened for inhibitory activity against Pd. In total, we obtained 1,362 bacterial isolates from 265 wild bats of 13 species in western Canada. Among the 1,362 isolates, 96 showed inhibitory activity against Pd based on a coculture assay. The inhibitory activities varied widely among these isolates, ranging from slowing fungal growth to complete inhibition. Interestingly, host bats containing isolates with anti-Pd activities were widely distributed, with no apparent geographic or species-specific pattern. However, characteristics of roosting sites and host demography showed significant associations with the isolation of anti-Pd bacteria. Specifically, anthropogenic roosts and swabs from young males had higher frequencies of anti-Pd bacteria than those from natural roosts and those from other sex and age-groups, respectively. These anti-Pd bacteria could be potentially used to help mitigate the impact of WNS. Field trials using these as well as additional microbes from future screenings are needed in order to determine their effectiveness for the prevention and treatment against WNS.https://doi.org/10.1038/s41598-022-14223-9 |
| spellingShingle | Adrian Forsythe Nick Fontaine Julianna Bissonnette Brandon Hayashi Chadabhorn Insuk Soumya Ghosh Gabrielle Kam Aaron Wong Cori Lausen Jianping Xu Naowarat Cheeptham Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings Scientific Reports |
| title | Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings |
| title_full | Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings |
| title_fullStr | Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings |
| title_full_unstemmed | Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings |
| title_short | Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings |
| title_sort | microbial isolates with anti pseudogymnoascus destructans activities from western canadian bat wings |
| url | https://doi.org/10.1038/s41598-022-14223-9 |
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