Ribbon scanning confocal for high-speed high-resolution volume imaging of brain.
Whole-brain imaging is becoming a fundamental means of experimental insight; however, achieving subcellular resolution imagery in a reasonable time window has not been possible. We describe the first application of multicolor ribbon scanning confocal methods to collect high-resolution volume images...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5501561?pdf=render |
| _version_ | 1831755475318734848 |
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| author | Alan M Watson Annika H Rose Gregory A Gibson Christina L Gardner Chengqun Sun Douglas S Reed L K Metthew Lam Claudette M St Croix Peter L Strick William B Klimstra Simon C Watkins |
| author_facet | Alan M Watson Annika H Rose Gregory A Gibson Christina L Gardner Chengqun Sun Douglas S Reed L K Metthew Lam Claudette M St Croix Peter L Strick William B Klimstra Simon C Watkins |
| author_sort | Alan M Watson |
| collection | DOAJ |
| description | Whole-brain imaging is becoming a fundamental means of experimental insight; however, achieving subcellular resolution imagery in a reasonable time window has not been possible. We describe the first application of multicolor ribbon scanning confocal methods to collect high-resolution volume images of chemically cleared brains. We demonstrate that ribbon scanning collects images over ten times faster than conventional high speed confocal systems but with equivalent spectral and spatial resolution. Further, using this technology, we reconstruct large volumes of mouse brain infected with encephalitic alphaviruses and demonstrate that regions of the brain with abundant viral replication were inaccessible to vascular perfusion. This reveals that the destruction or collapse of large regions of brain micro vasculature may contribute to the severe disease caused by Venezuelan equine encephalitis virus. Visualization of this fundamental impact of infection would not be possible without sampling at subcellular resolution within large brain volumes. |
| first_indexed | 2024-12-21T23:56:13Z |
| format | Article |
| id | doaj.art-0e6afc151b5a45e0a619bd14bbc2f3f3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T23:56:13Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0e6afc151b5a45e0a619bd14bbc2f3f32022-12-21T18:45:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018048610.1371/journal.pone.0180486Ribbon scanning confocal for high-speed high-resolution volume imaging of brain.Alan M WatsonAnnika H RoseGregory A GibsonChristina L GardnerChengqun SunDouglas S ReedL K Metthew LamClaudette M St CroixPeter L StrickWilliam B KlimstraSimon C WatkinsWhole-brain imaging is becoming a fundamental means of experimental insight; however, achieving subcellular resolution imagery in a reasonable time window has not been possible. We describe the first application of multicolor ribbon scanning confocal methods to collect high-resolution volume images of chemically cleared brains. We demonstrate that ribbon scanning collects images over ten times faster than conventional high speed confocal systems but with equivalent spectral and spatial resolution. Further, using this technology, we reconstruct large volumes of mouse brain infected with encephalitic alphaviruses and demonstrate that regions of the brain with abundant viral replication were inaccessible to vascular perfusion. This reveals that the destruction or collapse of large regions of brain micro vasculature may contribute to the severe disease caused by Venezuelan equine encephalitis virus. Visualization of this fundamental impact of infection would not be possible without sampling at subcellular resolution within large brain volumes.http://europepmc.org/articles/PMC5501561?pdf=render |
| spellingShingle | Alan M Watson Annika H Rose Gregory A Gibson Christina L Gardner Chengqun Sun Douglas S Reed L K Metthew Lam Claudette M St Croix Peter L Strick William B Klimstra Simon C Watkins Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. PLoS ONE |
| title | Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. |
| title_full | Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. |
| title_fullStr | Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. |
| title_full_unstemmed | Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. |
| title_short | Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. |
| title_sort | ribbon scanning confocal for high speed high resolution volume imaging of brain |
| url | http://europepmc.org/articles/PMC5501561?pdf=render |
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