Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system
Three-dimensional (3D) cell culture systems are reported to be more physiologically similar to the in vivo state than 2-dimensional (2D) models, which are extensively employed in periodontal research. Herein, we developed a 3D gingival tissue model with both epithelial and lamina propria layers usin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790222002173 |
| _version_ | 1797862155414929408 |
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| author | Yusuke Kurosawa Hirofumi Yamaguchi Kazuki Uemichi Keiji Shinozuka Yuki Kirihara Hiromasa Tsuda |
| author_facet | Yusuke Kurosawa Hirofumi Yamaguchi Kazuki Uemichi Keiji Shinozuka Yuki Kirihara Hiromasa Tsuda |
| author_sort | Yusuke Kurosawa |
| collection | DOAJ |
| description | Three-dimensional (3D) cell culture systems are reported to be more physiologically similar to the in vivo state than 2-dimensional (2D) models, which are extensively employed in periodontal research. Herein, we developed a 3D gingival tissue model with both epithelial and lamina propria layers using human gingival epithelial Ca9-22 cells and primary gingival fibroblasts. The epithelial layer of the developed 3D gingival tissue culture was treated with butyrate, a metabolite of oral bacteria, and the treatment induced the release of damage-associated molecular patterns, such as DNA and Sin3A associated protein 130 kDa (SAP130). Taken together, butyrate exposure to the epithelium of 3D gingival epithelial-connective tissue hybrid systems could induce epithelial cell death and the subsequent release of damage-associated molecular patterns. |
| first_indexed | 2024-04-09T22:14:41Z |
| format | Article |
| id | doaj.art-0e70517bef0f49dbb4016375200cd618 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-09T22:14:41Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-0e70517bef0f49dbb4016375200cd6182023-03-23T04:35:01ZengElsevierJournal of Dental Sciences1991-79022023-04-01182893897Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture systemYusuke Kurosawa0Hirofumi Yamaguchi1Kazuki Uemichi2Keiji Shinozuka3Yuki Kirihara4Hiromasa Tsuda5Nihon University School of Dentistry, Tokyo, JapanNihon University School of Dentistry, Tokyo, JapanDivision of Oral Structural and Functional Biology, Nihon University Graduate School of Dentistry, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, JapanDepartment of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, JapanDivision of Oral Structural and Functional Biology, Nihon University Graduate School of Dentistry, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, JapanDepartment of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan; Corresponding author. Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda-surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.Three-dimensional (3D) cell culture systems are reported to be more physiologically similar to the in vivo state than 2-dimensional (2D) models, which are extensively employed in periodontal research. Herein, we developed a 3D gingival tissue model with both epithelial and lamina propria layers using human gingival epithelial Ca9-22 cells and primary gingival fibroblasts. The epithelial layer of the developed 3D gingival tissue culture was treated with butyrate, a metabolite of oral bacteria, and the treatment induced the release of damage-associated molecular patterns, such as DNA and Sin3A associated protein 130 kDa (SAP130). Taken together, butyrate exposure to the epithelium of 3D gingival epithelial-connective tissue hybrid systems could induce epithelial cell death and the subsequent release of damage-associated molecular patterns.http://www.sciencedirect.com/science/article/pii/S1991790222002173Three-dimensional gingival tissue cultureButyrateDamage-associated molecular patternsCellular death |
| spellingShingle | Yusuke Kurosawa Hirofumi Yamaguchi Kazuki Uemichi Keiji Shinozuka Yuki Kirihara Hiromasa Tsuda Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system Journal of Dental Sciences Three-dimensional gingival tissue culture Butyrate Damage-associated molecular patterns Cellular death |
| title | Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system |
| title_full | Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system |
| title_fullStr | Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system |
| title_full_unstemmed | Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system |
| title_short | Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system |
| title_sort | butyrate treatment induces gingival epithelial cell death in a three dimensional gingival connective tissue hybrid co culture system |
| topic | Three-dimensional gingival tissue culture Butyrate Damage-associated molecular patterns Cellular death |
| url | http://www.sciencedirect.com/science/article/pii/S1991790222002173 |
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