3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity
The growing demand for personalized medicine requires innovation in drug manufacturing to combine versatility with automation. Here, three-dimensional (3D) printing was explored for the production of chitosan (CH)/alginate (ALG)-based hydrogels intended as active dressings for wound healing. ALG hyd...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/1/137 |
| _version_ | 1797438568100003840 |
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| author | Carlo Bergonzi Annalisa Bianchera Giulia Remaggi Maria Cristina Ossiprandi Ruggero Bettini Lisa Elviri |
| author_facet | Carlo Bergonzi Annalisa Bianchera Giulia Remaggi Maria Cristina Ossiprandi Ruggero Bettini Lisa Elviri |
| author_sort | Carlo Bergonzi |
| collection | DOAJ |
| description | The growing demand for personalized medicine requires innovation in drug manufacturing to combine versatility with automation. Here, three-dimensional (3D) printing was explored for the production of chitosan (CH)/alginate (ALG)-based hydrogels intended as active dressings for wound healing. ALG hydrogels were loaded with 0.75% w/v silver sulfadiazine (SSD), selected as a drug model commonly used for the therapeutic treatment of infected burn wounds, and four different 3D CH/ALG architectures were designed to modulate the release of this active compound. CH/ALG constructs were characterized by their water content, elasticity and porosity. ALG hydrogels (Young’s modulus 0.582 ± 0.019 Mpa) were statistically different in terms of elasticity compared to CH (Young’s modulus 0.365 ± 0.015 Mpa) but very similar in terms of swelling properties (water content in ALG: 93.18 ± 0.88% and in CH: 92.76 ± 1.17%). In vitro SSD release tests were performed by using vertical diffusion Franz cells, and statistically significant different behaviors in terms of the amount and kinetics of drugs released were observed as a function of the construct. Moreover, strong antimicrobial potency (100% of growth inhibition) against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> was demonstrated depending on the type of construct, offering a proof of concept that 3D printing techniques could be efficiently applied to the production of hydrogels for controlled drug delivery. |
| first_indexed | 2024-03-09T11:39:52Z |
| format | Article |
| id | doaj.art-0e7355cb035846beaa1963728f9c9158 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T11:39:52Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-0e7355cb035846beaa1963728f9c91582023-11-30T23:33:43ZengMDPI AGMicromachines2072-666X2023-01-0114113710.3390/mi140101373D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial ActivityCarlo Bergonzi0Annalisa Bianchera1Giulia Remaggi2Maria Cristina Ossiprandi3Ruggero Bettini4Lisa Elviri5Food and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyThe growing demand for personalized medicine requires innovation in drug manufacturing to combine versatility with automation. Here, three-dimensional (3D) printing was explored for the production of chitosan (CH)/alginate (ALG)-based hydrogels intended as active dressings for wound healing. ALG hydrogels were loaded with 0.75% w/v silver sulfadiazine (SSD), selected as a drug model commonly used for the therapeutic treatment of infected burn wounds, and four different 3D CH/ALG architectures were designed to modulate the release of this active compound. CH/ALG constructs were characterized by their water content, elasticity and porosity. ALG hydrogels (Young’s modulus 0.582 ± 0.019 Mpa) were statistically different in terms of elasticity compared to CH (Young’s modulus 0.365 ± 0.015 Mpa) but very similar in terms of swelling properties (water content in ALG: 93.18 ± 0.88% and in CH: 92.76 ± 1.17%). In vitro SSD release tests were performed by using vertical diffusion Franz cells, and statistically significant different behaviors in terms of the amount and kinetics of drugs released were observed as a function of the construct. Moreover, strong antimicrobial potency (100% of growth inhibition) against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> was demonstrated depending on the type of construct, offering a proof of concept that 3D printing techniques could be efficiently applied to the production of hydrogels for controlled drug delivery.https://www.mdpi.com/2072-666X/14/1/137chitosanalginatesilver sulfadiazine3D printed hydrogelsantimicrobial activitywound healing |
| spellingShingle | Carlo Bergonzi Annalisa Bianchera Giulia Remaggi Maria Cristina Ossiprandi Ruggero Bettini Lisa Elviri 3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity Micromachines chitosan alginate silver sulfadiazine 3D printed hydrogels antimicrobial activity wound healing |
| title | 3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity |
| title_full | 3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity |
| title_fullStr | 3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity |
| title_full_unstemmed | 3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity |
| title_short | 3D Printed Chitosan/Alginate Hydrogels for the Controlled Release of Silver Sulfadiazine in Wound Healing Applications: Design, Characterization and Antimicrobial Activity |
| title_sort | 3d printed chitosan alginate hydrogels for the controlled release of silver sulfadiazine in wound healing applications design characterization and antimicrobial activity |
| topic | chitosan alginate silver sulfadiazine 3D printed hydrogels antimicrobial activity wound healing |
| url | https://www.mdpi.com/2072-666X/14/1/137 |
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