Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke
Increasing evidence suggests that features of the gut microbiota correlate with ischemic stroke. However, the specific characteristics of the gut microbiota in patients suffering different types of ischemic stroke, or recovering from such strokes, have rarely been studied, and potential microbiotic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2020.587284/full |
| _version_ | 1818838904587943936 |
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| author | Lei Xiang Yanfeng Lou Lingyu Liu Yuanling Liu Weizheng Zhang Jianxin Deng Yubin Guan Miaoqin She Xinchao You Minqi Liu Hongwei Li Xiaosong Xu Fang Liu Xiangsheng Cai Xiangsheng Cai |
| author_facet | Lei Xiang Yanfeng Lou Lingyu Liu Yuanling Liu Weizheng Zhang Jianxin Deng Yubin Guan Miaoqin She Xinchao You Minqi Liu Hongwei Li Xiaosong Xu Fang Liu Xiangsheng Cai Xiangsheng Cai |
| author_sort | Lei Xiang |
| collection | DOAJ |
| description | Increasing evidence suggests that features of the gut microbiota correlate with ischemic stroke. However, the specific characteristics of the gut microbiota in patients suffering different types of ischemic stroke, or recovering from such strokes, have rarely been studied, and potential microbiotic predictors of different types of stroke have seldom been analyzed. We subjected fecal specimens from patients with lacunar or non-lacunar acute ischemic infarctions, and those recovering from such strokes, to bacterial 16S rRNA sequencing and compared the results to those of healthy volunteers. We identified microbial markers of different types of ischemic stroke and verified that these were of diagnostic utility. Patients with two types of ischemic stroke, and those recovering from ischemic stroke, exhibited significant shifts in microbiotic diversities compared to healthy subjects. Cluster of Orthologous Groups of Proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed reduced metabolic and transport-related pathway activities in ischemic stroke patients. We performed fivefold cross-validation using a Random Forest model to identify two optimal bacterial species (operational taxonomic units; OTUs) serving as markers of lacunar infarction; these were Lachnospiraceae (OTU_45) and Bacteroides (OTU_4), and the areas under the receiver operating characteristic curves (AUCs under the ROCs) were 0.881 and 0.872 respectively. In terms of non-lacunar acute ischemic infarction detection, the two optimal species were Bilophila (OTU_330) and Lachnospiraceae (OTU_338); the AUCs under the ROCs were 0.985 and 0.929 respectively. In post-ischemic stroke patients, the three optimal species were Pseudomonas (OTU_35), Sphingomonadaceae (OTU_303), and Akkermansia (OTU_9); the AUCs under the ROCs were 1, 0.897, and 0.846 respectively. Notably, the gut microbial markers were of considerable value for utility when diagnosing lacunar infarction, non-lacunar acute ischemic infarction, and post-ischemic stroke. This study is the first to characterize the gut microbiotic profiles of patients with lacunar or non-lacunar, acute ischemic strokes, and those recovering from stroke, and to identify microbiotic predictors of such strokes. |
| first_indexed | 2024-12-19T03:45:48Z |
| format | Article |
| id | doaj.art-0e74c36623e54d42bec63ec6e4652331 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-19T03:45:48Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0e74c36623e54d42bec63ec6e46523312022-12-21T20:37:07ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-12-011010.3389/fcimb.2020.587284587284Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic StrokeLei Xiang0Yanfeng Lou1Lingyu Liu2Yuanling Liu3Weizheng Zhang4Jianxin Deng5Yubin Guan6Miaoqin She7Xinchao You8Minqi Liu9Hongwei Li10Xiaosong Xu11Fang Liu12Xiangsheng Cai13Xiangsheng Cai14Department of Integrative Chinese and Western Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, ChinaDepartment of Dermatology, Jinling Hospital, Southern Medical University, Nanjing, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, ChinaAdministrative Department, Guangdong Province Hospital for Women and Children Healthcare, Guangzhou, ChinaClinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Endocrinology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center of Shenzhen University, Shenzhen, ChinaClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, ChinaResearch Section, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, ChinaGuangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China0Institute of Biotherapy, Southern Medical University, Guangzhou, ChinaClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, ChinaDepartment of Dermatology, Jinling Hospital, Southern Medical University, Nanjing, ChinaClinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China1Center for Medical Experiments, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, ChinaIncreasing evidence suggests that features of the gut microbiota correlate with ischemic stroke. However, the specific characteristics of the gut microbiota in patients suffering different types of ischemic stroke, or recovering from such strokes, have rarely been studied, and potential microbiotic predictors of different types of stroke have seldom been analyzed. We subjected fecal specimens from patients with lacunar or non-lacunar acute ischemic infarctions, and those recovering from such strokes, to bacterial 16S rRNA sequencing and compared the results to those of healthy volunteers. We identified microbial markers of different types of ischemic stroke and verified that these were of diagnostic utility. Patients with two types of ischemic stroke, and those recovering from ischemic stroke, exhibited significant shifts in microbiotic diversities compared to healthy subjects. Cluster of Orthologous Groups of Proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed reduced metabolic and transport-related pathway activities in ischemic stroke patients. We performed fivefold cross-validation using a Random Forest model to identify two optimal bacterial species (operational taxonomic units; OTUs) serving as markers of lacunar infarction; these were Lachnospiraceae (OTU_45) and Bacteroides (OTU_4), and the areas under the receiver operating characteristic curves (AUCs under the ROCs) were 0.881 and 0.872 respectively. In terms of non-lacunar acute ischemic infarction detection, the two optimal species were Bilophila (OTU_330) and Lachnospiraceae (OTU_338); the AUCs under the ROCs were 0.985 and 0.929 respectively. In post-ischemic stroke patients, the three optimal species were Pseudomonas (OTU_35), Sphingomonadaceae (OTU_303), and Akkermansia (OTU_9); the AUCs under the ROCs were 1, 0.897, and 0.846 respectively. Notably, the gut microbial markers were of considerable value for utility when diagnosing lacunar infarction, non-lacunar acute ischemic infarction, and post-ischemic stroke. This study is the first to characterize the gut microbiotic profiles of patients with lacunar or non-lacunar, acute ischemic strokes, and those recovering from stroke, and to identify microbiotic predictors of such strokes.https://www.frontiersin.org/articles/10.3389/fcimb.2020.587284/fulllacunar infarctionpost-ischemic strokegut microbiotaacute ischemic infarctionnon-lacunar acute ischemic infarctionrandom forest model |
| spellingShingle | Lei Xiang Yanfeng Lou Lingyu Liu Yuanling Liu Weizheng Zhang Jianxin Deng Yubin Guan Miaoqin She Xinchao You Minqi Liu Hongwei Li Xiaosong Xu Fang Liu Xiangsheng Cai Xiangsheng Cai Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke Frontiers in Cellular and Infection Microbiology lacunar infarction post-ischemic stroke gut microbiota acute ischemic infarction non-lacunar acute ischemic infarction random forest model |
| title | Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke |
| title_full | Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke |
| title_fullStr | Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke |
| title_full_unstemmed | Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke |
| title_short | Gut Microbiotic Features Aiding the Diagnosis of Acute Ischemic Stroke |
| title_sort | gut microbiotic features aiding the diagnosis of acute ischemic stroke |
| topic | lacunar infarction post-ischemic stroke gut microbiota acute ischemic infarction non-lacunar acute ischemic infarction random forest model |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2020.587284/full |
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