Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens
Basophils and mast cells have high affinity IgE receptors (FcεRI) on their plasma membrane and play important roles in FcεRI-associated allergic diseases, such as pollen allergy, food allergy, chronic spontaneous urticarial (CSU), and atopic dermatitis (AD). To date, several...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/20/1/45 |
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| author | Yuhki Yanase Yoshimi Matsuo Tomoko Kawaguchi Kaori Ishii Akio Tanaka Kazumasa Iwamoto Shunsuke Takahagi Michihiro Hide |
| author_facet | Yuhki Yanase Yoshimi Matsuo Tomoko Kawaguchi Kaori Ishii Akio Tanaka Kazumasa Iwamoto Shunsuke Takahagi Michihiro Hide |
| author_sort | Yuhki Yanase |
| collection | DOAJ |
| description | Basophils and mast cells have high affinity IgE receptors (FcεRI) on their plasma membrane and play important roles in FcεRI-associated allergic diseases, such as pollen allergy, food allergy, chronic spontaneous urticarial (CSU), and atopic dermatitis (AD). To date, several reports have revealed that high IgE antibody concentrations activate mast cells—which reside in tissue—in the absence of any antigens (allergens). However, IgE antibody-induced activation of basophils—which circulate in blood—has not been reported. Here, we investigated whether IgE antibodies may regulate functions of human peripheral basophils without antigens in vitro. We successfully removed IgE antibodies bound to FcεRI on the surface of human peripheral basophils by treating with 0.1% lactic acid. We also demonstrated that high IgE antibody concentrations (>1 μM) induced histamine release, polarization, and CD203c upregulation of IgE antibody-stripped basophils. Thus, high IgE antibody concentrations directly activate basophils, which express IgE-free FcεRI on the cell surface. This mechanism may contribute to the pathogenesis of patients with AD and CSU who have higher serum IgE concentrations compared to healthy donors. |
| first_indexed | 2024-04-12T17:35:27Z |
| format | Article |
| id | doaj.art-0e74e7acbc5a4aefacc5e476e20f53a0 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T17:35:27Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0e74e7acbc5a4aefacc5e476e20f53a02022-12-22T03:22:59ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-12-012014510.3390/ijms20010045ijms20010045Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without AntigensYuhki Yanase0Yoshimi Matsuo1Tomoko Kawaguchi2Kaori Ishii3Akio Tanaka4Kazumasa Iwamoto5Shunsuke Takahagi6Michihiro Hide7Department of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Dermatology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanBasophils and mast cells have high affinity IgE receptors (FcεRI) on their plasma membrane and play important roles in FcεRI-associated allergic diseases, such as pollen allergy, food allergy, chronic spontaneous urticarial (CSU), and atopic dermatitis (AD). To date, several reports have revealed that high IgE antibody concentrations activate mast cells—which reside in tissue—in the absence of any antigens (allergens). However, IgE antibody-induced activation of basophils—which circulate in blood—has not been reported. Here, we investigated whether IgE antibodies may regulate functions of human peripheral basophils without antigens in vitro. We successfully removed IgE antibodies bound to FcεRI on the surface of human peripheral basophils by treating with 0.1% lactic acid. We also demonstrated that high IgE antibody concentrations (>1 μM) induced histamine release, polarization, and CD203c upregulation of IgE antibody-stripped basophils. Thus, high IgE antibody concentrations directly activate basophils, which express IgE-free FcεRI on the cell surface. This mechanism may contribute to the pathogenesis of patients with AD and CSU who have higher serum IgE concentrations compared to healthy donors.http://www.mdpi.com/1422-0067/20/1/45basophilsIgE antibodyFcεRIhistamineCD203c |
| spellingShingle | Yuhki Yanase Yoshimi Matsuo Tomoko Kawaguchi Kaori Ishii Akio Tanaka Kazumasa Iwamoto Shunsuke Takahagi Michihiro Hide Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens International Journal of Molecular Sciences basophils IgE antibody FcεRI histamine CD203c |
| title | Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens |
| title_full | Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens |
| title_fullStr | Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens |
| title_full_unstemmed | Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens |
| title_short | Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens |
| title_sort | activation of human peripheral basophils in response to high ige antibody concentrations without antigens |
| topic | basophils IgE antibody FcεRI histamine CD203c |
| url | http://www.mdpi.com/1422-0067/20/1/45 |
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