A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model
Escherichia coli (E. coli) is a major bacterial pathogen associated with many cases of serious infections, such as urinary tract infections (UTI) and meningitis intestinal. The rapid emergence of antimicrobial multidrug-resistant bacteria occurring worldwide has been attributed to the overuse of ant...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2021-11-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_124947_ed280f97d0ced0f2088369e533b609fd.pdf |
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| author | B Mijbel Ali S. A Gatea Kaabi M. A Al-Bayati H. K Musafer |
| author_facet | B Mijbel Ali S. A Gatea Kaabi M. A Al-Bayati H. K Musafer |
| author_sort | B Mijbel Ali |
| collection | DOAJ |
| description | Escherichia coli (E. coli) is a major bacterial pathogen associated with many cases of serious infections, such as urinary tract infections (UTI) and meningitis intestinal. The rapid emergence of antimicrobial multidrug-resistant bacteria occurring worldwide has been attributed to the overuse of antibiotics. Alternative strategies must be developed to overcome antibiotic resistance. A promising alternative for the treatment of infections is the use of phages as antibacterial agents. A total of 90 female albino mice were randomly divided into three groups (n=30) and used for the induction of UTI. The animals were acclimatized in their cages for 24 h before inoculation and allowed to access chow and water freely. For UTI induction, the peri-urethral area was sterilized with 70% ethanol, and bacterial inoculation was then injected into the bladder through the urethra using a 24-gauge sterile Teflon catheter with an outer diameter of 0.7 mm and length of 19 mm. A single phage and a phage cocktail preparation have been evaluated for their therapeutic activity in the mouse model of chronic UTI induced by transurethral injection of two isolates of the uropathogenic E. coli 8 and E. coli 302. The results of the transurethral and intra-peritoneal injection of phage(s) that prepared on day 10 after the establishment of the mouse chronic model showed no effect of a single phage PEC80 in the treatment of UTI, whereas both administration routes of the phage cocktail preparation resulted in the clearance of bacteria from mice urine and homogenates of the urinary bladders and kidneys of the sacrificed mice after 24 h following the administration of phage cocktail dose. The high activity of the phage cocktail in the treatment of mouse chronic model of UTI is attributed to the broader host range of the phage cocktail, compared to the very narrow host range of the phage PEC80. It is concluded that the phage therapy by using phage preparations as the 25 phages cocktail evaluated in this study is a highly promising and potential alternative therapy for human UTIs. |
| first_indexed | 2024-12-12T14:40:33Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-12T14:40:33Z |
| publishDate | 2021-11-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-0e76a4ede9894b70b5c55b3f06bad59c2022-12-22T00:21:14ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-11-017651229123610.22092/ari.2021.356004.1762124947A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse ModelB Mijbel Ali0S. A Gatea Kaabi1M. A Al-Bayati2H. K Musafer3Department of Biology, College of Sciences, Mustansiriyah University, Baghdad, IraqDepartment of Biology, College of Sciences, Mustansiriyah University, Baghdad, IraqDepartment of Biology, College of Sciences, Mustansiriyah University, Baghdad, IraqDepartment of Biology, College of Sciences, Mustansiriyah University, Baghdad, IraqEscherichia coli (E. coli) is a major bacterial pathogen associated with many cases of serious infections, such as urinary tract infections (UTI) and meningitis intestinal. The rapid emergence of antimicrobial multidrug-resistant bacteria occurring worldwide has been attributed to the overuse of antibiotics. Alternative strategies must be developed to overcome antibiotic resistance. A promising alternative for the treatment of infections is the use of phages as antibacterial agents. A total of 90 female albino mice were randomly divided into three groups (n=30) and used for the induction of UTI. The animals were acclimatized in their cages for 24 h before inoculation and allowed to access chow and water freely. For UTI induction, the peri-urethral area was sterilized with 70% ethanol, and bacterial inoculation was then injected into the bladder through the urethra using a 24-gauge sterile Teflon catheter with an outer diameter of 0.7 mm and length of 19 mm. A single phage and a phage cocktail preparation have been evaluated for their therapeutic activity in the mouse model of chronic UTI induced by transurethral injection of two isolates of the uropathogenic E. coli 8 and E. coli 302. The results of the transurethral and intra-peritoneal injection of phage(s) that prepared on day 10 after the establishment of the mouse chronic model showed no effect of a single phage PEC80 in the treatment of UTI, whereas both administration routes of the phage cocktail preparation resulted in the clearance of bacteria from mice urine and homogenates of the urinary bladders and kidneys of the sacrificed mice after 24 h following the administration of phage cocktail dose. The high activity of the phage cocktail in the treatment of mouse chronic model of UTI is attributed to the broader host range of the phage cocktail, compared to the very narrow host range of the phage PEC80. It is concluded that the phage therapy by using phage preparations as the 25 phages cocktail evaluated in this study is a highly promising and potential alternative therapy for human UTIs.https://archrazi.areeo.ac.ir/article_124947_ed280f97d0ced0f2088369e533b609fd.pdfphage therapyutidrug resistancephage cocktailalternative therapy |
| spellingShingle | B Mijbel Ali S. A Gatea Kaabi M. A Al-Bayati H. K Musafer A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model Archives of Razi Institute phage therapy uti drug resistance phage cocktail alternative therapy |
| title | A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model |
| title_full | A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model |
| title_fullStr | A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model |
| title_full_unstemmed | A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model |
| title_short | A Novel Phage Cocktail Therapy of the Urinary Tract Infection in a Mouse Model |
| title_sort | novel phage cocktail therapy of the urinary tract infection in a mouse model |
| topic | phage therapy uti drug resistance phage cocktail alternative therapy |
| url | https://archrazi.areeo.ac.ir/article_124947_ed280f97d0ced0f2088369e533b609fd.pdf |
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