Genetic polymorphism of local Abkhazian grape cultivars
Local grape cultivars from different countries of the world are an important part of the gene pool of this culture. Of particular interest are the genotypes of the most ancient regions of viticulture. The territories of the subtropical zone of Georgia and the central part of Abkhazia belong to one o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2022-01-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/3197 |
| _version_ | 1797213989726322688 |
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| author | E. T. Ilnitskaya M. V. Makarkina I. V. Stepanov I. I. Suprun S. V. Tokmakov V. Ch. Aiba M. A. Avidzba V. K. Kotlyar |
| author_facet | E. T. Ilnitskaya M. V. Makarkina I. V. Stepanov I. I. Suprun S. V. Tokmakov V. Ch. Aiba M. A. Avidzba V. K. Kotlyar |
| author_sort | E. T. Ilnitskaya |
| collection | DOAJ |
| description | Local grape cultivars from different countries of the world are an important part of the gene pool of this culture. Of particular interest are the genotypes of the most ancient regions of viticulture. The territories of the subtropical zone of Georgia and the central part of Abkhazia belong to one of the centers of origin of the cultural grapevine. The purpose of the work was to genotype native Abkhazian grape cultivars, to study their genetic diversity based on DNA profiling data and to compare them with the genotypes of local varieties of other viticultural regions. Samples of plants were taken on the territory of the Republic of Abkhazia in private farmsteads and in the collection of the agricultural firm “Vina i Vody Abkhazii“ (“Wines and Waters of Abkhazia”). The genotyping of the Abkhazian cultivars Avasirhva, Agbizh, Azhapsh, Azhizhkvakva, Azhikvaca, Atvizh, Atyrkuazh, Achkykazh, Kachich was carried out using 14 DNA markers, 9 of which are standard microsatellite markers recommended for the identification of grape varieties. To improve our knowledge about the sizes of the identified alleles, we used the DNA of grape cultivars with a known allelic composition at the analyzed loci. Statistical analysis of the data showed that the observed heterozygosity for the analyzed loci exceeded expected values, which indicates a genetic polymorphism of the studied sample of varieties. Evaluation of genetic similarity within the analyzed group based on the results of genotyping at 14 loci showed that the cultivars Kachich and Azhapsh differed from the other Abkhazian varieties. The obtained DNA profiles of the Abkhazian cultivars were checked for compliance with DNA-fingerprints of grape varieties in the Vitis International Variety Catalogue. The Georgian varieties Azhizhkvakva and Tsitska turned out to be synonyms according to DNA profiles, two varieties from the Database (Italian Albana bianca and Georgian Ojaleshi) have differences in DNA-fingerprints from the varieties Atyrkuazh and Azhikvatsa only in one allele, respectively. When comparing the identified Abkhazian grape genotypes, their difference from the sample of Dagestan, Don, Greek, Turkish, Italian, Spanish, and French varieties and genetic similarity with the genotypes of Georgian grapes were shown. |
| first_indexed | 2024-03-07T16:04:43Z |
| format | Article |
| id | doaj.art-0e7761abb1e34fd6a4c287ca27049d47 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:07:03Z |
| publishDate | 2022-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-0e7761abb1e34fd6a4c287ca27049d472024-04-11T15:31:04ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592022-01-0125879780410.18699/VJ21.0921217Genetic polymorphism of local Abkhazian grape cultivarsE. T. Ilnitskaya0M. V. Makarkina1I. V. Stepanov2I. I. Suprun3S. V. Tokmakov4V. Ch. Aiba5M. A. Avidzba6V. K. Kotlyar7North-Caucasian Federal Scientific Center of Horticulture, Viticulture, WinemakingNorth-Caucasian Federal Scientific Center of Horticulture, Viticulture, WinemakingNorth-Caucasian Federal Scientific Center of Horticulture, Viticulture, WinemakingNorth-Caucasian Federal Scientific Center of Horticulture, Viticulture, WinemakingNorth-Caucasian Federal Scientific Center of Horticulture, Viticulture, WinemakingInstitute of Agriculture of the Academy of Sciences of AbkhaziaInstitute of Agriculture of the Academy of Sciences of AbkhaziaNorth-Caucasian Federal Scientific Center of Horticulture, Viticulture, WinemakingLocal grape cultivars from different countries of the world are an important part of the gene pool of this culture. Of particular interest are the genotypes of the most ancient regions of viticulture. The territories of the subtropical zone of Georgia and the central part of Abkhazia belong to one of the centers of origin of the cultural grapevine. The purpose of the work was to genotype native Abkhazian grape cultivars, to study their genetic diversity based on DNA profiling data and to compare them with the genotypes of local varieties of other viticultural regions. Samples of plants were taken on the territory of the Republic of Abkhazia in private farmsteads and in the collection of the agricultural firm “Vina i Vody Abkhazii“ (“Wines and Waters of Abkhazia”). The genotyping of the Abkhazian cultivars Avasirhva, Agbizh, Azhapsh, Azhizhkvakva, Azhikvaca, Atvizh, Atyrkuazh, Achkykazh, Kachich was carried out using 14 DNA markers, 9 of which are standard microsatellite markers recommended for the identification of grape varieties. To improve our knowledge about the sizes of the identified alleles, we used the DNA of grape cultivars with a known allelic composition at the analyzed loci. Statistical analysis of the data showed that the observed heterozygosity for the analyzed loci exceeded expected values, which indicates a genetic polymorphism of the studied sample of varieties. Evaluation of genetic similarity within the analyzed group based on the results of genotyping at 14 loci showed that the cultivars Kachich and Azhapsh differed from the other Abkhazian varieties. The obtained DNA profiles of the Abkhazian cultivars were checked for compliance with DNA-fingerprints of grape varieties in the Vitis International Variety Catalogue. The Georgian varieties Azhizhkvakva and Tsitska turned out to be synonyms according to DNA profiles, two varieties from the Database (Italian Albana bianca and Georgian Ojaleshi) have differences in DNA-fingerprints from the varieties Atyrkuazh and Azhikvatsa only in one allele, respectively. When comparing the identified Abkhazian grape genotypes, their difference from the sample of Dagestan, Don, Greek, Turkish, Italian, Spanish, and French varieties and genetic similarity with the genotypes of Georgian grapes were shown.https://vavilov.elpub.ru/jour/article/view/3197vitis vinifera l.local grape varietiesgenetic diversityssr-loci |
| spellingShingle | E. T. Ilnitskaya M. V. Makarkina I. V. Stepanov I. I. Suprun S. V. Tokmakov V. Ch. Aiba M. A. Avidzba V. K. Kotlyar Genetic polymorphism of local Abkhazian grape cultivars Вавиловский журнал генетики и селекции vitis vinifera l. local grape varieties genetic diversity ssr-loci |
| title | Genetic polymorphism of local Abkhazian grape cultivars |
| title_full | Genetic polymorphism of local Abkhazian grape cultivars |
| title_fullStr | Genetic polymorphism of local Abkhazian grape cultivars |
| title_full_unstemmed | Genetic polymorphism of local Abkhazian grape cultivars |
| title_short | Genetic polymorphism of local Abkhazian grape cultivars |
| title_sort | genetic polymorphism of local abkhazian grape cultivars |
| topic | vitis vinifera l. local grape varieties genetic diversity ssr-loci |
| url | https://vavilov.elpub.ru/jour/article/view/3197 |
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