| Summary: | <i>Yersinia pseudotuberculosis</i> is an extracellular foodborne pathogen and usually causes self-limiting diarrhea in healthy humans. MgtC is known as a key subversion factor that contributes to intramacrophage adaptation and intracellular survival in certain important pathogens. Whether MgtC influences the fitness of <i>Y. pseudotuberculosis</i> is unclear. According to in silico analysis, MgtC in <i>Y. pseudotuberculosis</i> might share similar functions with other bacterial pathogens, such as <i>Salmonella</i>. Studies indicated that MgtC was clearly required for <i>Y. pseudotuberculosis</i> growth in vitro and bacterial survival in macrophages under Mg<sup>2+</sup> starvation. Transcriptome analysis by RNA-seq indicated that 127 differentially expressed genes (DEGs) (fold change > 2 and <i>p</i> < 0.001) were discovered between wild-type PB1+ and <i>mgtC</i> mutant inside macrophages. However, a lack of MgtC only moderately, albeit significantly, reduced the virulence of <i>Y. pseudotuberculosis</i> in mice. Overall, this study provides additional insights for the role of MgtC in <i>Y. pseudotuberculosis</i>.
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