C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice
Occupational medicamentose-like dermatitis due to trichloroethylene (OMDT) is a key but unresolved question. OMDT patients often present multiple organ damage, including kidney damage. However, the underlying mechanism remains unknown. The purpose of our study was to explore the effect of tubule-spe...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322008600 |
| _version_ | 1798031380078133248 |
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| author | Zhibing Liu Jinru Ma Xulei Zuo Xuesong Zhang Yiting Hong Shuyang Cai Hua Huang Feng Wang Changhao Wu Jiaxiang Zhang Qixing Zhu |
| author_facet | Zhibing Liu Jinru Ma Xulei Zuo Xuesong Zhang Yiting Hong Shuyang Cai Hua Huang Feng Wang Changhao Wu Jiaxiang Zhang Qixing Zhu |
| author_sort | Zhibing Liu |
| collection | DOAJ |
| description | Occupational medicamentose-like dermatitis due to trichloroethylene (OMDT) is a key but unresolved question. OMDT patients often present multiple organ damage, including kidney damage. However, the underlying mechanism remains unknown. The purpose of our study was to explore the effect of tubule-specific C5b-9 deposition induced by TCE sensitization on renal tubular ferroptosis and its mechanism. By analyzing pathological changes of TCE-sensitization-mice kidney, we observed a significant renal tubular ferroptosis, which was alleviated by CD59, a C5b-9 inhibitory protein. Moreover, this phenomenon was also replicated in a C5b-9-attacked HK-2 cell model. Further experiments identified that C5b-9 induced cytosolic Ca2+ overload in renal tubular epithelia cells from TCE-sensitization-mice and HK-2 cells. Furthermore, in vitro experiments showed that BAPTA-AM, an intracellular Ca2+ chelator, could rescued ferroptosis induced by C5b-9 in HK-2 cells. Taken together, TCE sensitization induced renal tubular ferroptosis is mediated by C5b-9 and cytosolic Ca2+ overload may play a key role. |
| first_indexed | 2024-04-11T19:56:34Z |
| format | Article |
| id | doaj.art-0e7cf4ae0620469695c8d4419d981152 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T19:56:34Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-0e7cf4ae0620469695c8d4419d9811522022-12-22T04:06:00ZengElsevierEcotoxicology and Environmental Safety0147-65132022-10-01244114020C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization miceZhibing Liu0Jinru Ma1Xulei Zuo2Xuesong Zhang3Yiting Hong4Shuyang Cai5Hua Huang6Feng Wang7Changhao Wu8Jiaxiang Zhang9Qixing Zhu10Department of Dermatology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui, ChinaDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, ChinaDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, ChinaDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, ChinaDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, ChinaDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, ChinaDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, ChinaDepartment of Dermatology, Second Hospital of Anhui Medical University, Hefei, Anhui, ChinaSchool of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UKDepartment of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Corresponding author.Department of Dermatology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui, China; Corresponding author at: Department of Dermatology, First Affiliated Hospital of Anhui Medical, University, Hefei, Anhui, China.Occupational medicamentose-like dermatitis due to trichloroethylene (OMDT) is a key but unresolved question. OMDT patients often present multiple organ damage, including kidney damage. However, the underlying mechanism remains unknown. The purpose of our study was to explore the effect of tubule-specific C5b-9 deposition induced by TCE sensitization on renal tubular ferroptosis and its mechanism. By analyzing pathological changes of TCE-sensitization-mice kidney, we observed a significant renal tubular ferroptosis, which was alleviated by CD59, a C5b-9 inhibitory protein. Moreover, this phenomenon was also replicated in a C5b-9-attacked HK-2 cell model. Further experiments identified that C5b-9 induced cytosolic Ca2+ overload in renal tubular epithelia cells from TCE-sensitization-mice and HK-2 cells. Furthermore, in vitro experiments showed that BAPTA-AM, an intracellular Ca2+ chelator, could rescued ferroptosis induced by C5b-9 in HK-2 cells. Taken together, TCE sensitization induced renal tubular ferroptosis is mediated by C5b-9 and cytosolic Ca2+ overload may play a key role.http://www.sciencedirect.com/science/article/pii/S0147651322008600OMDTC5b-9Renal tubulesCa2+ overloadFerroptosis |
| spellingShingle | Zhibing Liu Jinru Ma Xulei Zuo Xuesong Zhang Yiting Hong Shuyang Cai Hua Huang Feng Wang Changhao Wu Jiaxiang Zhang Qixing Zhu C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice Ecotoxicology and Environmental Safety OMDT C5b-9 Renal tubules Ca2+ overload Ferroptosis |
| title | C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice |
| title_full | C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice |
| title_fullStr | C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice |
| title_full_unstemmed | C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice |
| title_short | C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice |
| title_sort | c5b 9 mediates ferroptosis of tubular epithelial cells in trichloroethylene sensitization mice |
| topic | OMDT C5b-9 Renal tubules Ca2+ overload Ferroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322008600 |
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