The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran
Background: Genetic variants affect the expression of microRNAs (miRNA) resulted in changes in the expression levels of the miRNA target genes that may lead to the increased risk of cancer. The aim of the current study was to investigate the association between the common single-nucleotide gene poly...
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Isfahan University of Medical Sciences
2019-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/11900 |
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| author | Manijeh Vaheb Modjtaba Emadi-Baygi Nastaran Injinari Hossein Teimori |
| author_facet | Manijeh Vaheb Modjtaba Emadi-Baygi Nastaran Injinari Hossein Teimori |
| author_sort | Manijeh Vaheb |
| collection | DOAJ |
| description | Background: Genetic variants affect the expression of microRNAs (miRNA) resulted in changes in the expression levels of the miRNA target genes that may lead to the increased risk of cancer. The aim of the current study was to investigate the association between the common single-nucleotide gene polymorphism T < C (rs11614913) in MIR196A2 and the risk of developing prostate cancer (PCa) in Chahar Mahal and Bakhtiari Province, Iran.
Methods: In this case-control study, 99 patients were enrolled in the case group and 100 healthy controls in the control group. From all participants, 5 ml venous blood sample was collected and DNA was extracted by phenol-chloroform extraction. Specific primers were designed for rs11614913 polymorphism, and this polymorphism was genotyped using High Resolution Melt (HRM) analysis. Hardy-Weinberg equilibrium for the genotypes was tested by chi-squared test. The association between different genotypes and the risk of prostate cancer was investigated by logistic regression analysis.
Findings: Among 99 patients, 68 had prostate cancer and 31 had benign prostatic hyperplasia (BPH). Logistic regression analysis showed that those cases with CC genotype had a higher risk of developing prostate cancer [Odds ratio (OR) = 1.08] than T (TT+CT) carriers. Allele frequency analysis revealed that the C allele increased the risk of developing prostate cancer and BPH 1.3 more than T allele.
Conclusion: The C allele in rs11614913 polymorphism increases the risk of prostate cancer. Because this polymorphism is present in MIR196A2 gene, evaluation of expression of the miRNA gene and its target genes in patients with prostate cancer could be valuable. |
| first_indexed | 2024-03-12T09:27:54Z |
| format | Article |
| id | doaj.art-0e7e6386808440229b2db14a15d2733d |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:27:54Z |
| publishDate | 2019-08-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-0e7e6386808440229b2db14a15d2733d2023-09-02T14:03:43ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2019-08-013752753554110.22122/jims.v37i527.119003422The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, IranManijeh Vaheb0Modjtaba Emadi-Baygi1Nastaran Injinari2Hossein Teimori3Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranAssociate Professor, Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, IranMSc Student, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranAssociate Professor, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranBackground: Genetic variants affect the expression of microRNAs (miRNA) resulted in changes in the expression levels of the miRNA target genes that may lead to the increased risk of cancer. The aim of the current study was to investigate the association between the common single-nucleotide gene polymorphism T < C (rs11614913) in MIR196A2 and the risk of developing prostate cancer (PCa) in Chahar Mahal and Bakhtiari Province, Iran. Methods: In this case-control study, 99 patients were enrolled in the case group and 100 healthy controls in the control group. From all participants, 5 ml venous blood sample was collected and DNA was extracted by phenol-chloroform extraction. Specific primers were designed for rs11614913 polymorphism, and this polymorphism was genotyped using High Resolution Melt (HRM) analysis. Hardy-Weinberg equilibrium for the genotypes was tested by chi-squared test. The association between different genotypes and the risk of prostate cancer was investigated by logistic regression analysis. Findings: Among 99 patients, 68 had prostate cancer and 31 had benign prostatic hyperplasia (BPH). Logistic regression analysis showed that those cases with CC genotype had a higher risk of developing prostate cancer [Odds ratio (OR) = 1.08] than T (TT+CT) carriers. Allele frequency analysis revealed that the C allele increased the risk of developing prostate cancer and BPH 1.3 more than T allele. Conclusion: The C allele in rs11614913 polymorphism increases the risk of prostate cancer. Because this polymorphism is present in MIR196A2 gene, evaluation of expression of the miRNA gene and its target genes in patients with prostate cancer could be valuable.http://jims.mui.ac.ir/index.php/jims/article/view/11900GenesProstate cancerMicroRNAsGenetic polymorphism |
| spellingShingle | Manijeh Vaheb Modjtaba Emadi-Baygi Nastaran Injinari Hossein Teimori The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran مجله دانشکده پزشکی اصفهان Genes Prostate cancer MicroRNAs Genetic polymorphism |
| title | The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran |
| title_full | The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran |
| title_fullStr | The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran |
| title_full_unstemmed | The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran |
| title_short | The Evaluation of rs11614913 Polymorphism in MIR196A2 Gene and the Risk of Prostate Cancer in Chaharmahal and Bakhtiari Province, Iran |
| title_sort | evaluation of rs11614913 polymorphism in mir196a2 gene and the risk of prostate cancer in chaharmahal and bakhtiari province iran |
| topic | Genes Prostate cancer MicroRNAs Genetic polymorphism |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/11900 |
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