Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls
Heat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bul...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-06-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201506-0003_association-of-heat-shock-protein-70-with-motility-of-frozen-thawed-sperm-in-bulls.php |
| _version_ | 1797899167209619456 |
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| author | X.-G. Zhang J.-Y. Hong G.-J. Yan Y.-F. Wang Q.-W. Li J.-H. Hu |
| author_facet | X.-G. Zhang J.-Y. Hong G.-J. Yan Y.-F. Wang Q.-W. Li J.-H. Hu |
| author_sort | X.-G. Zhang |
| collection | DOAJ |
| description | Heat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bulls by artificial vagina. Sperm motility and plasma membrane integrity of the semen samples at three stages (fresh, after equilibration, and frozen-thawed) were evaluated. The HSP70 expression level at the three stages was detected using real-time PCR. The results indicated that HSP70 expression level, membrane integrity, and sperm motility in the fresh semen were higher than those of the sperm after equilibration and freezing-thawing (P < 0.05), the HSP70 expression level, plasma membrane integrity, and sperm motility in sperm after equilibration were higher than those of the frozen-thawed sperm (P < 0.05). The correlation between HSP70 expression level and sperm motility was positive (ranging from 0.327 to 0.785). The results suggest that HSP70 expression level in bull spermatozoa was gradually decreased following the process of freezing-thawing, and might be associated with bull sperm motility. |
| first_indexed | 2024-04-10T08:25:44Z |
| format | Article |
| id | doaj.art-0e7e968500474a2c8f685365c205fe09 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:44Z |
| publishDate | 2015-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-0e7e968500474a2c8f685365c205fe092023-02-23T03:33:18ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092015-06-0160625626210.17221/8239-CJAScjs-201506-0003Association of heat shock protein 70 with motility of frozen-thawed sperm in bullsX.-G. Zhang0J.-Y. Hong1G.-J. Yan2Y.-F. Wang3Q.-W. Li4J.-H. Hu5College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaHeat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bulls by artificial vagina. Sperm motility and plasma membrane integrity of the semen samples at three stages (fresh, after equilibration, and frozen-thawed) were evaluated. The HSP70 expression level at the three stages was detected using real-time PCR. The results indicated that HSP70 expression level, membrane integrity, and sperm motility in the fresh semen were higher than those of the sperm after equilibration and freezing-thawing (P < 0.05), the HSP70 expression level, plasma membrane integrity, and sperm motility in sperm after equilibration were higher than those of the frozen-thawed sperm (P < 0.05). The correlation between HSP70 expression level and sperm motility was positive (ranging from 0.327 to 0.785). The results suggest that HSP70 expression level in bull spermatozoa was gradually decreased following the process of freezing-thawing, and might be associated with bull sperm motility.https://cjas.agriculturejournals.cz/artkey/cjs-201506-0003_association-of-heat-shock-protein-70-with-motility-of-frozen-thawed-sperm-in-bulls.phpgene expressioncryopreservationhsp70sperm motility |
| spellingShingle | X.-G. Zhang J.-Y. Hong G.-J. Yan Y.-F. Wang Q.-W. Li J.-H. Hu Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls Czech Journal of Animal Science gene expression cryopreservation hsp70 sperm motility |
| title | Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls |
| title_full | Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls |
| title_fullStr | Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls |
| title_full_unstemmed | Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls |
| title_short | Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls |
| title_sort | association of heat shock protein 70 with motility of frozen thawed sperm in bulls |
| topic | gene expression cryopreservation hsp70 sperm motility |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201506-0003_association-of-heat-shock-protein-70-with-motility-of-frozen-thawed-sperm-in-bulls.php |
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