Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream
Abstract Purpose: To evaluate the inflammatory reaction and measure the content of mucins, in the colonic mucosa without fecal stream submit to intervention with mesalazine. Methods: Twenty-four rats were submitted to a left colostomy and a distal mucous fistula and divided into two groups accord...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2019-04-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/acb/v34n4/1678-2674-acb-34-04-e201900406.pdf |
| _version_ | 1818887020214222848 |
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| author | Carlos Augusto Real Martinez Fábio Guilherme Campos Danilo Toshio Kanno Eli Cristiano Meneses Gabrielle Maira Matijascic Eduardo Felipe Kim Goto José Aires Pereira |
| author_facet | Carlos Augusto Real Martinez Fábio Guilherme Campos Danilo Toshio Kanno Eli Cristiano Meneses Gabrielle Maira Matijascic Eduardo Felipe Kim Goto José Aires Pereira |
| author_sort | Carlos Augusto Real Martinez |
| collection | DOAJ |
| description | Abstract Purpose: To evaluate the inflammatory reaction and measure the content of mucins, in the colonic mucosa without fecal stream submit to intervention with mesalazine. Methods: Twenty-four rats were submitted to a left colostomy and a distal mucous fistula and divided into two groups according to euthanasia to be performed two or four weeks. Each group was divided into two subgroups according daily application of enemas containing saline or mesalazine at 1.0 g/kg/day. Colitis was diagnosed by histological analysis and the inflammatory reaction by validated score. Acidic mucins and neutral mucins were determined with the alcian-blue and periodic acid of Schiff techniques, respectively. Sulfomucin and sialomucin were identified by high iron diamine-alcian blue technique. The tissue contents of mucins were quantified by computer-assisted image analysis. Mann-Whitney test was used to analyze the results establishing the level of significance of 5%. Results: Enemas with mesalazine in colonic segments without fecal stream decreased the inflammation score and increased the tissue content of all subtypes of mucins. The increase of tissue content of neutral, acid and sulfomucin was related to the time of intervention. Conclusion: Mesalazine enemas reduce the inflammatory process and preserve the content of mucins in colonic mucosa devoid of fecal stream. |
| first_indexed | 2024-12-19T16:30:35Z |
| format | Article |
| id | doaj.art-0e7f269bd08849a1b9751aa05b8cd22d |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-19T16:30:35Z |
| publishDate | 2019-04-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-0e7f269bd08849a1b9751aa05b8cd22d2022-12-21T20:14:12ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502019-04-0134410.1590/s0102-865020190040000006Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal streamCarlos Augusto Real Martinezhttps://orcid.org/0000-0001-8088-427XFábio Guilherme Camposhttps://orcid.org/0000-0002-6233-6904Danilo Toshio Kannohttps://orcid.org/0000-0001-7120-4017Eli Cristiano Meneseshttps://orcid.org/0000-0003-4395-0623Gabrielle Maira Matijascichttps://orcid.org/0000-0002-9270-1837Eduardo Felipe Kim Gotohttps://orcid.org/0000-0002-1218-1035José Aires Pereirahttps://orcid.org/0000-0003-3937-7182Abstract Purpose: To evaluate the inflammatory reaction and measure the content of mucins, in the colonic mucosa without fecal stream submit to intervention with mesalazine. Methods: Twenty-four rats were submitted to a left colostomy and a distal mucous fistula and divided into two groups according to euthanasia to be performed two or four weeks. Each group was divided into two subgroups according daily application of enemas containing saline or mesalazine at 1.0 g/kg/day. Colitis was diagnosed by histological analysis and the inflammatory reaction by validated score. Acidic mucins and neutral mucins were determined with the alcian-blue and periodic acid of Schiff techniques, respectively. Sulfomucin and sialomucin were identified by high iron diamine-alcian blue technique. The tissue contents of mucins were quantified by computer-assisted image analysis. Mann-Whitney test was used to analyze the results establishing the level of significance of 5%. Results: Enemas with mesalazine in colonic segments without fecal stream decreased the inflammation score and increased the tissue content of all subtypes of mucins. The increase of tissue content of neutral, acid and sulfomucin was related to the time of intervention. Conclusion: Mesalazine enemas reduce the inflammatory process and preserve the content of mucins in colonic mucosa devoid of fecal stream.http://www.scielo.br/pdf/acb/v34n4/1678-2674-acb-34-04-e201900406.pdfMesalamineColitisFatty Acids, VolatileMucinsImage Processing, Computer-AssistedRats |
| spellingShingle | Carlos Augusto Real Martinez Fábio Guilherme Campos Danilo Toshio Kanno Eli Cristiano Meneses Gabrielle Maira Matijascic Eduardo Felipe Kim Goto José Aires Pereira Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream Acta Cirúrgica Brasileira Mesalamine Colitis Fatty Acids, Volatile Mucins Image Processing, Computer-Assisted Rats |
| title | Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream |
| title_full | Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream |
| title_fullStr | Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream |
| title_full_unstemmed | Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream |
| title_short | Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream |
| title_sort | enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream |
| topic | Mesalamine Colitis Fatty Acids, Volatile Mucins Image Processing, Computer-Assisted Rats |
| url | http://www.scielo.br/pdf/acb/v34n4/1678-2674-acb-34-04-e201900406.pdf |
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