Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans
Lipidomics provides an overview of lipid profiles in biological systems. Although blood is commonly used for lipid profiling, cerebrospinal fluid (CSF) is more suitable for exploring lipid homeostasis in brain diseases. However, whether an individual’s background affects the CSF lipid profile remain...
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MDPI AG
2021-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/5/268 |
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| author | Kosuke Saito Kotaro Hattori Shinsuke Hidese Daimei Sasayama Tomoko Miyakawa Ryo Matsumura Megumi Tatsumi Yuuki Yokota Miho Ota Hiroaki Hori Hiroshi Kunugi |
| author_facet | Kosuke Saito Kotaro Hattori Shinsuke Hidese Daimei Sasayama Tomoko Miyakawa Ryo Matsumura Megumi Tatsumi Yuuki Yokota Miho Ota Hiroaki Hori Hiroshi Kunugi |
| author_sort | Kosuke Saito |
| collection | DOAJ |
| description | Lipidomics provides an overview of lipid profiles in biological systems. Although blood is commonly used for lipid profiling, cerebrospinal fluid (CSF) is more suitable for exploring lipid homeostasis in brain diseases. However, whether an individual’s background affects the CSF lipid profile remains unclear, and the association between CSF and plasma lipid profiles in heathy individuals has not yet been defined. Herein, lipidomics approaches were employed to analyze CSF and plasma samples obtained from 114 healthy Japanese subjects. Results showed that the global lipid profiles differed significantly between CSF and plasma, with only 13 of 114 lipids found to be significantly correlated between the two matrices. Additionally, the CSF total protein content was the primary factor associated with CSF lipids. In the CSF, the levels of major lipids, namely, phosphatidylcholines, sphingomyelins, and cholesterolesters, correlated with CSF total protein levels. These findings indicate that CSF lipidomics can be applied to explore changes in lipid homeostasis in patients with brain diseases. |
| first_indexed | 2024-03-10T12:00:30Z |
| format | Article |
| id | doaj.art-0e819405ca82443c888a68e7e5d1b6ab |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T12:00:30Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-0e819405ca82443c888a68e7e5d1b6ab2023-11-21T16:56:54ZengMDPI AGMetabolites2218-19892021-04-0111526810.3390/metabo11050268Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy HumansKosuke Saito0Kotaro Hattori1Shinsuke Hidese2Daimei Sasayama3Tomoko Miyakawa4Ryo Matsumura5Megumi Tatsumi6Yuuki Yokota7Miho Ota8Hiroaki Hori9Hiroshi Kunugi10Division of Medical Safety Science, National Institute of Health Sciences, Kanagawa 210-9501, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanMedical Genome Center, National Center of Neurology and Psychiatry, Tokyo 187-8551, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, JapanLipidomics provides an overview of lipid profiles in biological systems. Although blood is commonly used for lipid profiling, cerebrospinal fluid (CSF) is more suitable for exploring lipid homeostasis in brain diseases. However, whether an individual’s background affects the CSF lipid profile remains unclear, and the association between CSF and plasma lipid profiles in heathy individuals has not yet been defined. Herein, lipidomics approaches were employed to analyze CSF and plasma samples obtained from 114 healthy Japanese subjects. Results showed that the global lipid profiles differed significantly between CSF and plasma, with only 13 of 114 lipids found to be significantly correlated between the two matrices. Additionally, the CSF total protein content was the primary factor associated with CSF lipids. In the CSF, the levels of major lipids, namely, phosphatidylcholines, sphingomyelins, and cholesterolesters, correlated with CSF total protein levels. These findings indicate that CSF lipidomics can be applied to explore changes in lipid homeostasis in patients with brain diseases.https://www.mdpi.com/2218-1989/11/5/268cerebrospinal fluidlipidomicslipid profilingmass spectrometryplasma lipid |
| spellingShingle | Kosuke Saito Kotaro Hattori Shinsuke Hidese Daimei Sasayama Tomoko Miyakawa Ryo Matsumura Megumi Tatsumi Yuuki Yokota Miho Ota Hiroaki Hori Hiroshi Kunugi Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans Metabolites cerebrospinal fluid lipidomics lipid profiling mass spectrometry plasma lipid |
| title | Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans |
| title_full | Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans |
| title_fullStr | Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans |
| title_full_unstemmed | Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans |
| title_short | Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans |
| title_sort | profiling of cerebrospinal fluid lipids and their relationship with plasma lipids in healthy humans |
| topic | cerebrospinal fluid lipidomics lipid profiling mass spectrometry plasma lipid |
| url | https://www.mdpi.com/2218-1989/11/5/268 |
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