3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions
The immobilization of enzymes in biocatalytic flow reactors is a common strategy to increase enzyme reusability and improve biocatalytic performance. Extrusion-based 3D bioprinting has recently emerged as a versatile tool for the fabrication of perfusable hydrogel grids containing entrapped enzymes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00713/full |
| _version_ | 1818403713093468160 |
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| author | Lukas Wenger Lukas Wenger Carsten P. Radtke Jacqueline Göpper Michael Wörner Jürgen Hubbuch Jürgen Hubbuch |
| author_facet | Lukas Wenger Lukas Wenger Carsten P. Radtke Jacqueline Göpper Michael Wörner Jürgen Hubbuch Jürgen Hubbuch |
| author_sort | Lukas Wenger |
| collection | DOAJ |
| description | The immobilization of enzymes in biocatalytic flow reactors is a common strategy to increase enzyme reusability and improve biocatalytic performance. Extrusion-based 3D bioprinting has recently emerged as a versatile tool for the fabrication of perfusable hydrogel grids containing entrapped enzymes for the use in such reactors. This study demonstrates the suitability of water-in-oil high internal phase emulsions (HIPEs) as 3D-printable bioinks for the fabrication of composite materials with a porous polymeric scaffold (polyHIPE) filled with enzyme-laden hydrogel. The prepared HIPEs exhibited excellent printability and are shown to be suitable for the printing of complex three-dimensional structures without the need for sacrificial support material. An automated activity assay method for the systematic screening of different material compositions in small-scale batch experiments is presented. The monomer mass fraction in the aqueous phase and the thickness of printed objects were found to be the most important parameters determining the apparent activity of the immobilized enzyme. Mass transfer limitations and enzyme inactivation were identified as probable factors reducing the apparent activity. The presented HIPE-based bioinks enable the fabrication of flow-optimized and more efficient biocatalytic reactors while the automated activity assay method allows the rapid screening of materials to optimize the biocatalytic efficiency further without time-consuming flow-through experiments involving whole printed reactors. |
| first_indexed | 2024-12-14T08:28:38Z |
| format | Article |
| id | doaj.art-0e830b0f02154c2a8aacff7623276e36 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T08:28:38Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-0e830b0f02154c2a8aacff7623276e362022-12-21T23:09:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-07-01810.3389/fbioe.2020.007135444393D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase EmulsionsLukas Wenger0Lukas Wenger1Carsten P. Radtke2Jacqueline Göpper3Michael Wörner4Jürgen Hubbuch5Jürgen Hubbuch6Institute of Functional Interfaces, Department of Bioengineering and Biosystems, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, GermanyInstitute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute of Functional Interfaces, Department of Bioengineering and Biosystems, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, GermanyInstitute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Karlsruhe, GermanyThe immobilization of enzymes in biocatalytic flow reactors is a common strategy to increase enzyme reusability and improve biocatalytic performance. Extrusion-based 3D bioprinting has recently emerged as a versatile tool for the fabrication of perfusable hydrogel grids containing entrapped enzymes for the use in such reactors. This study demonstrates the suitability of water-in-oil high internal phase emulsions (HIPEs) as 3D-printable bioinks for the fabrication of composite materials with a porous polymeric scaffold (polyHIPE) filled with enzyme-laden hydrogel. The prepared HIPEs exhibited excellent printability and are shown to be suitable for the printing of complex three-dimensional structures without the need for sacrificial support material. An automated activity assay method for the systematic screening of different material compositions in small-scale batch experiments is presented. The monomer mass fraction in the aqueous phase and the thickness of printed objects were found to be the most important parameters determining the apparent activity of the immobilized enzyme. Mass transfer limitations and enzyme inactivation were identified as probable factors reducing the apparent activity. The presented HIPE-based bioinks enable the fabrication of flow-optimized and more efficient biocatalytic reactors while the automated activity assay method allows the rapid screening of materials to optimize the biocatalytic efficiency further without time-consuming flow-through experiments involving whole printed reactors.https://www.frontiersin.org/article/10.3389/fbioe.2020.00713/full3D printingbioprintingcure-on-dispensehydrogelsenzymesbeta-galactosidase |
| spellingShingle | Lukas Wenger Lukas Wenger Carsten P. Radtke Jacqueline Göpper Michael Wörner Jürgen Hubbuch Jürgen Hubbuch 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions Frontiers in Bioengineering and Biotechnology 3D printing bioprinting cure-on-dispense hydrogels enzymes beta-galactosidase |
| title | 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions |
| title_full | 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions |
| title_fullStr | 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions |
| title_full_unstemmed | 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions |
| title_short | 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions |
| title_sort | 3d printable and enzymatically active composite materials based on hydrogel filled high internal phase emulsions |
| topic | 3D printing bioprinting cure-on-dispense hydrogels enzymes beta-galactosidase |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00713/full |
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