A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions
A comprehensive approach using phenomics and global transcriptomics for dissecting plant response to biostimulants is illustrated with tomato (Solanum lycopersicum cv. Micro-Tom and Rio Grande) plants cultivated in the laboratory, greenhouse, and open field conditions. Biostimulant treatment based o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.983772/full |
| _version_ | 1828379747851173888 |
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| author | Ali Baghdadi Maria Cristina Della Lucia Matteo Borella Giovanni Bertoldo Samathmika Ravi Walter Zegada-Lizarazu Claudia Chiodi Elena Pagani Christian Hermans Piergiorgio Stevanato Serenella Nardi Andrea Monti Francesca Mangione |
| author_facet | Ali Baghdadi Maria Cristina Della Lucia Matteo Borella Giovanni Bertoldo Samathmika Ravi Walter Zegada-Lizarazu Claudia Chiodi Elena Pagani Christian Hermans Piergiorgio Stevanato Serenella Nardi Andrea Monti Francesca Mangione |
| author_sort | Ali Baghdadi |
| collection | DOAJ |
| description | A comprehensive approach using phenomics and global transcriptomics for dissecting plant response to biostimulants is illustrated with tomato (Solanum lycopersicum cv. Micro-Tom and Rio Grande) plants cultivated in the laboratory, greenhouse, and open field conditions. Biostimulant treatment based on an Ascophyllum nodosum extract (ANE) was applied as a foliar spray with two doses (1 or 2 l ha-1) at three different phenological stages (BBCH51, BBCH61, and BBCH65) during the flowering phase. Both ANE doses resulted in greater net photosynthesis rate, stomatal conductance, and fruit yield across all culture conditions. A global transcriptomic analysis of leaves from plants grown in the climate chamber, revealed a greater number of differentially expressed genes (DEGs) with the low ANE dose compared to the greater one. The second and third applications induced broader transcriptome changes compared to the first one, indicating a cumulative treatment effect. The functional enrichment analysis of DEGs highlighted pathways related to stimulus-response and photosynthesis, consistent with the morpho-physiological observations. This study is the first comprehensive dual-omics approach for profiling plant responses to biostimulants across three different culture conditions. |
| first_indexed | 2024-12-10T03:45:12Z |
| format | Article |
| id | doaj.art-0e85091b05aa43ee827b3437c7fdb2c1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T03:45:12Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0e85091b05aa43ee827b3437c7fdb2c12022-12-22T02:03:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.983772983772A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditionsAli Baghdadi0Maria Cristina Della Lucia1Matteo Borella2Giovanni Bertoldo3Samathmika Ravi4Walter Zegada-Lizarazu5Claudia Chiodi6Elena Pagani7Christian Hermans8Piergiorgio Stevanato9Serenella Nardi10Andrea Monti11Francesca Mangione12Department of Agricultural and Food Sciences, University of Bologna, Bologna, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Legnaro, ItalyDepartment of Agricultural and Food Sciences, University of Bologna, Bologna, ItalyCrop Production and Biostimulation Laboratory, Brussels Bioengineering School, Université libre de Bruxelles, Brussels, BelgiumDepartment of Agricultural and Food Sciences, University of Bologna, Bologna, ItalyCrop Production and Biostimulation Laboratory, Brussels Bioengineering School, Université libre de Bruxelles, Brussels, BelgiumDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Legnaro, ItalyDepartment of Agricultural and Food Sciences, University of Bologna, Bologna, ItalySipcam Italia S.p.A. belonging together with Sofbey SA to the Sipcam Oxon S.p.A. Group, Pero, MI, ItalyA comprehensive approach using phenomics and global transcriptomics for dissecting plant response to biostimulants is illustrated with tomato (Solanum lycopersicum cv. Micro-Tom and Rio Grande) plants cultivated in the laboratory, greenhouse, and open field conditions. Biostimulant treatment based on an Ascophyllum nodosum extract (ANE) was applied as a foliar spray with two doses (1 or 2 l ha-1) at three different phenological stages (BBCH51, BBCH61, and BBCH65) during the flowering phase. Both ANE doses resulted in greater net photosynthesis rate, stomatal conductance, and fruit yield across all culture conditions. A global transcriptomic analysis of leaves from plants grown in the climate chamber, revealed a greater number of differentially expressed genes (DEGs) with the low ANE dose compared to the greater one. The second and third applications induced broader transcriptome changes compared to the first one, indicating a cumulative treatment effect. The functional enrichment analysis of DEGs highlighted pathways related to stimulus-response and photosynthesis, consistent with the morpho-physiological observations. This study is the first comprehensive dual-omics approach for profiling plant responses to biostimulants across three different culture conditions.https://www.frontiersin.org/articles/10.3389/fpls.2022.983772/fullAscophyllum nodosumbiostimulantcrop yieldplant physiologytomatotranscriptome |
| spellingShingle | Ali Baghdadi Maria Cristina Della Lucia Matteo Borella Giovanni Bertoldo Samathmika Ravi Walter Zegada-Lizarazu Claudia Chiodi Elena Pagani Christian Hermans Piergiorgio Stevanato Serenella Nardi Andrea Monti Francesca Mangione A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions Frontiers in Plant Science Ascophyllum nodosum biostimulant crop yield plant physiology tomato transcriptome |
| title | A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions |
| title_full | A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions |
| title_fullStr | A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions |
| title_full_unstemmed | A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions |
| title_short | A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions |
| title_sort | dual omics approach for profiling plant responses to biostimulant applications under controlled and field conditions |
| topic | Ascophyllum nodosum biostimulant crop yield plant physiology tomato transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.983772/full |
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