Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
Bovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preserv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/10/10/268 |
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| author | Mira Abou-Diab Jacinthe Thibodeau Barbara Deracinois Christophe Flahaut Ismail Fliss Pascal Dhulster Laurent Bazinet Naima Nedjar |
| author_facet | Mira Abou-Diab Jacinthe Thibodeau Barbara Deracinois Christophe Flahaut Ismail Fliss Pascal Dhulster Laurent Bazinet Naima Nedjar |
| author_sort | Mira Abou-Diab |
| collection | DOAJ |
| description | Bovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preservatives for the food sector. In this work, the hemoglobin hydrolysis to produce bioactive peptides was performed in a regulated pH medium without the use of chemical solvents and by an eco-efficient process: electrodialysis with bipolar membrane (EDBM). Bipolar/monopolar (anionic or cationic) configuration using the H<sup>+</sup> and OH<sup>−</sup> generated by the bipolar membranes to regulate the pH was investigated. The aim of this study was to present and identify the bioactive peptides produced by EDBM in comparison with conventional hydrolysis and to identify their biological activity. The use of the EDBM for the enzymatic hydrolysis of hemoglobin has allowed for the production and identification of 17 bioactive peptides. Hydrolysates obtained by EDBM showed an excellent antimicrobial activity against six strains, antioxidant activity measured by four different tests and for the first time anti-fungal activities against five yeasts and mold strains. Consequently, this enzymatic hydrolysis carried out in regulated pH medium with bipolar membranes could provide bioactive peptides presenting antibacterial, antifungal and antioxidant interest. |
| first_indexed | 2024-03-09T06:05:57Z |
| format | Article |
| id | doaj.art-0e85f6be5b184c4ab630ae863ec4ba8f |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T06:05:57Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-0e85f6be5b184c4ab630ae863ec4ba8f2023-12-03T12:03:40ZengMDPI AGMembranes2077-03752020-09-01101026810.3390/membranes10100268Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive PeptidesMira Abou-Diab0Jacinthe Thibodeau1Barbara Deracinois2Christophe Flahaut3Ismail Fliss4Pascal Dhulster5Laurent Bazinet6Naima Nedjar7Department of Food Science, Université Laval, Québec, QC G1V 0A6, CanadaDepartment of Food Science, Université Laval, Québec, QC G1V 0A6, CanadaUMR Transfrontalière BioEcoAgro N°1158, Université Lille, INRAE, Université Liège, UPJV, YNCREA, Université Artois, Université Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceUMR Transfrontalière BioEcoAgro N°1158, Université Lille, INRAE, Université Liège, UPJV, YNCREA, Université Artois, Université Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceDepartment of Food Science, Université Laval, Québec, QC G1V 0A6, CanadaUMR Transfrontalière BioEcoAgro N°1158, Université Lille, INRAE, Université Liège, UPJV, YNCREA, Université Artois, Université Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceDepartment of Food Science, Université Laval, Québec, QC G1V 0A6, CanadaUMR Transfrontalière BioEcoAgro N°1158, Université Lille, INRAE, Université Liège, UPJV, YNCREA, Université Artois, Université Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceBovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preservatives for the food sector. In this work, the hemoglobin hydrolysis to produce bioactive peptides was performed in a regulated pH medium without the use of chemical solvents and by an eco-efficient process: electrodialysis with bipolar membrane (EDBM). Bipolar/monopolar (anionic or cationic) configuration using the H<sup>+</sup> and OH<sup>−</sup> generated by the bipolar membranes to regulate the pH was investigated. The aim of this study was to present and identify the bioactive peptides produced by EDBM in comparison with conventional hydrolysis and to identify their biological activity. The use of the EDBM for the enzymatic hydrolysis of hemoglobin has allowed for the production and identification of 17 bioactive peptides. Hydrolysates obtained by EDBM showed an excellent antimicrobial activity against six strains, antioxidant activity measured by four different tests and for the first time anti-fungal activities against five yeasts and mold strains. Consequently, this enzymatic hydrolysis carried out in regulated pH medium with bipolar membranes could provide bioactive peptides presenting antibacterial, antifungal and antioxidant interest.https://www.mdpi.com/2077-0375/10/10/268bovine hemoglobinelectrodialysis with bipolar membranebioactive peptidesantibacterial activityantifungal activityantioxidant activity |
| spellingShingle | Mira Abou-Diab Jacinthe Thibodeau Barbara Deracinois Christophe Flahaut Ismail Fliss Pascal Dhulster Laurent Bazinet Naima Nedjar Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides Membranes bovine hemoglobin electrodialysis with bipolar membrane bioactive peptides antibacterial activity antifungal activity antioxidant activity |
| title | Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides |
| title_full | Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides |
| title_fullStr | Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides |
| title_full_unstemmed | Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides |
| title_short | Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides |
| title_sort | bovine hemoglobin enzymatic hydrolysis by a new eco efficient process part ii production of bioactive peptides |
| topic | bovine hemoglobin electrodialysis with bipolar membrane bioactive peptides antibacterial activity antifungal activity antioxidant activity |
| url | https://www.mdpi.com/2077-0375/10/10/268 |
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