The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat
Abstract Background The epithelial mesenchymal transition (EMT) plays a critical role in normal embryonic development as well as abnormal pathological events such as tumor formation and metastasis. One of these important events that may explain this phenomenon during embryogenesis is the migration o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-01-01
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| Series: | Journal of Basic and Applied Zoology |
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| Online Access: | https://doi.org/10.1186/s41936-019-0137-8 |
| _version_ | 1818598660609409024 |
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| author | Lina A. Aeshra Maiada Moustafa Mohammed I.Y. Elmallah Said Abdelrahman Salih Ibrahim Y. Abdel Kader |
| author_facet | Lina A. Aeshra Maiada Moustafa Mohammed I.Y. Elmallah Said Abdelrahman Salih Ibrahim Y. Abdel Kader |
| author_sort | Lina A. Aeshra |
| collection | DOAJ |
| description | Abstract Background The epithelial mesenchymal transition (EMT) plays a critical role in normal embryonic development as well as abnormal pathological events such as tumor formation and metastasis. One of these important events that may explain this phenomenon during embryogenesis is the migration of primordial germ cells (PGCs) from hindgut into the genital ridge during gonadogenesis. Yet, more studies are needed to explain the molecular changes underlying the role of EMT during gonadogenesis. Here, we aimed to study the molecular changes of four genes to determine whether the EMT occurs during gonadogenesis. These studied genes are alkaline phosphatase (ALP) as PGC marker, E-cadherin (CDH1) as epithelial marker, vimentin (VIM) as mesenchymal marker, and signal transducer and activator of transcription 3 (STAT3) as migration regulator and differentiation marker. Results The results showed a substantial decrease in the expression of ALP by the increase of the embryonic age. The expression of CDH1 is inversely proportional to the expression of VIM during the days of PGC migration. STAT3 expression related to the time of occurrence of migration and differentiation. Conclusion Taken together, our findings demonstrated the molecular changes of ALP, PGC, CDH1, and VIM during the process of gonadogenesis. Further studies are warranted to understand other molecular mechanisms associated with gonadogenesis. |
| first_indexed | 2024-12-16T12:07:14Z |
| format | Article |
| id | doaj.art-0e86fefc92ea470d98a40f6d578d5895 |
| institution | Directory Open Access Journal |
| issn | 2090-990X |
| language | English |
| last_indexed | 2024-12-16T12:07:14Z |
| publishDate | 2020-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj.art-0e86fefc92ea470d98a40f6d578d58952022-12-21T22:32:17ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2020-01-018111510.1186/s41936-019-0137-8The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino ratLina A. Aeshra0Maiada Moustafa1Mohammed I.Y. Elmallah2Said Abdelrahman Salih3Ibrahim Y. Abdel Kader4Chemistry Department, Faculty of Science, Cairo UniversityZoology and Entomology Department, Faculty of Science, Helwan UniversityChemistry Department, Faculty of ScienceChemistry Department, Faculty of Science, Cairo UniversityBritish UniversityAbstract Background The epithelial mesenchymal transition (EMT) plays a critical role in normal embryonic development as well as abnormal pathological events such as tumor formation and metastasis. One of these important events that may explain this phenomenon during embryogenesis is the migration of primordial germ cells (PGCs) from hindgut into the genital ridge during gonadogenesis. Yet, more studies are needed to explain the molecular changes underlying the role of EMT during gonadogenesis. Here, we aimed to study the molecular changes of four genes to determine whether the EMT occurs during gonadogenesis. These studied genes are alkaline phosphatase (ALP) as PGC marker, E-cadherin (CDH1) as epithelial marker, vimentin (VIM) as mesenchymal marker, and signal transducer and activator of transcription 3 (STAT3) as migration regulator and differentiation marker. Results The results showed a substantial decrease in the expression of ALP by the increase of the embryonic age. The expression of CDH1 is inversely proportional to the expression of VIM during the days of PGC migration. STAT3 expression related to the time of occurrence of migration and differentiation. Conclusion Taken together, our findings demonstrated the molecular changes of ALP, PGC, CDH1, and VIM during the process of gonadogenesis. Further studies are warranted to understand other molecular mechanisms associated with gonadogenesis.https://doi.org/10.1186/s41936-019-0137-8GonadogenesisEMTMETEmbryogenesisPGC migration |
| spellingShingle | Lina A. Aeshra Maiada Moustafa Mohammed I.Y. Elmallah Said Abdelrahman Salih Ibrahim Y. Abdel Kader The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat Journal of Basic and Applied Zoology Gonadogenesis EMT MET Embryogenesis PGC migration |
| title | The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat |
| title_full | The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat |
| title_fullStr | The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat |
| title_full_unstemmed | The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat |
| title_short | The role of epithelial–mesenchymal transition (EMT)-associated genes during gonadogenesis of albino rat |
| title_sort | role of epithelial mesenchymal transition emt associated genes during gonadogenesis of albino rat |
| topic | Gonadogenesis EMT MET Embryogenesis PGC migration |
| url | https://doi.org/10.1186/s41936-019-0137-8 |
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