Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia
ABSTRACT While Klebsiella pneumoniae is a common cause of nosocomial and community-acquired infections, including pneumonia and pyogenic liver abscess, little is known about the population structure of this bacterium. In this study, we investigated the prevalence and molecular characteristics of K....
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-04-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02646-21 |
| _version_ | 1811303417920880640 |
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| author | Lin Gan Chao Yan Jinghua Cui Guanhua Xue Hanyu Fu Bing Du Hanqing Zhao Junxia Feng Yanling Feng Zheng Fan Pan Mao Tongtong Fu Ziying Xu Shuheng Du Shiyu Liu Rui Zhang Qun Zhang Nannan Li Xiaohu Cui Xiaoran Li Yao Zhou Lei Huang Jing Yuan |
| author_facet | Lin Gan Chao Yan Jinghua Cui Guanhua Xue Hanyu Fu Bing Du Hanqing Zhao Junxia Feng Yanling Feng Zheng Fan Pan Mao Tongtong Fu Ziying Xu Shuheng Du Shiyu Liu Rui Zhang Qun Zhang Nannan Li Xiaohu Cui Xiaoran Li Yao Zhou Lei Huang Jing Yuan |
| author_sort | Lin Gan |
| collection | DOAJ |
| description | ABSTRACT While Klebsiella pneumoniae is a common cause of nosocomial and community-acquired infections, including pneumonia and pyogenic liver abscess, little is known about the population structure of this bacterium. In this study, we investigated the prevalence and molecular characteristics of K. pneumoniae isolates from carriers, pyogenic liver abscess patients, and pneumonia patients, and genomic and phenotypic assays were used to determine the differences among the isolates. A total of 232 K. pneumoniae isolates were subtyped into 74 sequence types (STs). The isolates from different sources had their own STs, and the predominant subtypes in liver abscess and pneumonia patients were ST23 and ST11, respectively. Pangenome analysis also distinguished three phylogroups that were consistent with the isolate sources. The isolates collected from liver abscess patients carried significantly more virulence factors, and those from pneumonia patients harbored significantly more resistance genes and replicons. Almost all isolate STs (93/97 [95.88%]) from liver abscesses strongly correlated with the virulence factor salmochelin, while most pneumonia isolate STs (52/53 [98.11%]) from pneumonia did not correlate with salmochelin. The isolates collected from liver abscesses showed higher virulence in the cytotoxicity and mouse models. These data provide genomic support for the proposal that isolates collected from carriers, liver abscess patients, and pneumonia patients have distinct genomic features. Isolates from the different sources are largely nonoverlapping, suggesting that different patients may be infected via different sources. Further studies on the pathogenic mechanisms of salmochelin and other virulence factors will be required. IMPORTANCE While Klebsiella pneumoniae is a common cause of nosocomial and community-acquired infections, including pneumonia and pyogenic liver abscess, little is known about the population structure of this bacterium. We collected 232 isolates from carriers, pyogenic liver abscess patients, and pneumonia patients, and the isolates from different sources had their own sequence types. Pangenome analysis also distinguished three phylogroups that were consistent with the isolate sources. The isolates collected from liver abscess patients carried significantly more virulence factors, and those from pneumonia patients harbored significantly more resistance genes and replicons. Besides, there was a strong link between salmochelin and liver abscess. The isolates collected from liver abscesses also showed higher virulence in the cytotoxicity and mouse models. Isolates collected from different sources have distinct genomic features, suggesting that different patients may be infected via different sources. |
| first_indexed | 2024-04-13T07:47:37Z |
| format | Article |
| id | doaj.art-0e89f4a1ae0f4dc09a3b560a5dbbda96 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-13T07:47:37Z |
| publishDate | 2022-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-0e89f4a1ae0f4dc09a3b560a5dbbda962022-12-22T02:55:38ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-04-0110210.1128/spectrum.02646-21Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and PneumoniaLin Gan0Chao Yan1Jinghua Cui2Guanhua Xue3Hanyu Fu4Bing Du5Hanqing Zhao6Junxia Feng7Yanling Feng8Zheng Fan9Pan Mao10Tongtong Fu11Ziying Xu12Shuheng Du13Shiyu Liu14Rui Zhang15Qun Zhang16Nannan Li17Xiaohu Cui18Xiaoran Li19Yao Zhou20Lei Huang21Jing Yuan22Department of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Disease Control and Prevention, Beijing, China Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaTreatment and Research Center for Infectious Diseases, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, ChinaABSTRACT While Klebsiella pneumoniae is a common cause of nosocomial and community-acquired infections, including pneumonia and pyogenic liver abscess, little is known about the population structure of this bacterium. In this study, we investigated the prevalence and molecular characteristics of K. pneumoniae isolates from carriers, pyogenic liver abscess patients, and pneumonia patients, and genomic and phenotypic assays were used to determine the differences among the isolates. A total of 232 K. pneumoniae isolates were subtyped into 74 sequence types (STs). The isolates from different sources had their own STs, and the predominant subtypes in liver abscess and pneumonia patients were ST23 and ST11, respectively. Pangenome analysis also distinguished three phylogroups that were consistent with the isolate sources. The isolates collected from liver abscess patients carried significantly more virulence factors, and those from pneumonia patients harbored significantly more resistance genes and replicons. Almost all isolate STs (93/97 [95.88%]) from liver abscesses strongly correlated with the virulence factor salmochelin, while most pneumonia isolate STs (52/53 [98.11%]) from pneumonia did not correlate with salmochelin. The isolates collected from liver abscesses showed higher virulence in the cytotoxicity and mouse models. These data provide genomic support for the proposal that isolates collected from carriers, liver abscess patients, and pneumonia patients have distinct genomic features. Isolates from the different sources are largely nonoverlapping, suggesting that different patients may be infected via different sources. Further studies on the pathogenic mechanisms of salmochelin and other virulence factors will be required. IMPORTANCE While Klebsiella pneumoniae is a common cause of nosocomial and community-acquired infections, including pneumonia and pyogenic liver abscess, little is known about the population structure of this bacterium. We collected 232 isolates from carriers, pyogenic liver abscess patients, and pneumonia patients, and the isolates from different sources had their own sequence types. Pangenome analysis also distinguished three phylogroups that were consistent with the isolate sources. The isolates collected from liver abscess patients carried significantly more virulence factors, and those from pneumonia patients harbored significantly more resistance genes and replicons. Besides, there was a strong link between salmochelin and liver abscess. The isolates collected from liver abscesses also showed higher virulence in the cytotoxicity and mouse models. Isolates collected from different sources have distinct genomic features, suggesting that different patients may be infected via different sources.https://journals.asm.org/doi/10.1128/spectrum.02646-21Klebsiella pneumoniaepyogenic liver abscesspneumoniavirulenceantibiotic resistance |
| spellingShingle | Lin Gan Chao Yan Jinghua Cui Guanhua Xue Hanyu Fu Bing Du Hanqing Zhao Junxia Feng Yanling Feng Zheng Fan Pan Mao Tongtong Fu Ziying Xu Shuheng Du Shiyu Liu Rui Zhang Qun Zhang Nannan Li Xiaohu Cui Xiaoran Li Yao Zhou Lei Huang Jing Yuan Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia Microbiology Spectrum Klebsiella pneumoniae pyogenic liver abscess pneumonia virulence antibiotic resistance |
| title | Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia |
| title_full | Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia |
| title_fullStr | Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia |
| title_full_unstemmed | Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia |
| title_short | Genetic Diversity and Pathogenic Features in Klebsiella pneumoniae Isolates from Patients with Pyogenic Liver Abscess and Pneumonia |
| title_sort | genetic diversity and pathogenic features in klebsiella pneumoniae isolates from patients with pyogenic liver abscess and pneumonia |
| topic | Klebsiella pneumoniae pyogenic liver abscess pneumonia virulence antibiotic resistance |
| url | https://journals.asm.org/doi/10.1128/spectrum.02646-21 |
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