Multivesicular release favors short term synaptic depression in hippocampal autapses
Presynaptic terminals of the central nervous system can support univesicular and multivesicular synchronous release of neurotransmitters, however, the functional implications of the prevalence of one mechanism over the other are yet unresolved. Here, we took advantage of the expression of SF-iGluSnF...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2023.1057242/full |
| _version_ | 1797825434868514816 |
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| author | Pablo Martínez San Segundo Pablo Martínez San Segundo Beatrice Terni Beatrice Terni Artur Llobet Artur Llobet |
| author_facet | Pablo Martínez San Segundo Pablo Martínez San Segundo Beatrice Terni Beatrice Terni Artur Llobet Artur Llobet |
| author_sort | Pablo Martínez San Segundo |
| collection | DOAJ |
| description | Presynaptic terminals of the central nervous system can support univesicular and multivesicular synchronous release of neurotransmitters, however, the functional implications of the prevalence of one mechanism over the other are yet unresolved. Here, we took advantage of the expression of SF-iGluSnFR.S72A in the astrocytic feeder layer of autaptic hippocampal neuronal cultures to associate the liberation of glutamate to excitatory postsynaptic currents. The presence of the glutamate sensor in glial cells avoided any interference with the function of endogenous postsynaptic receptors. It was possible to optically detect changes in neurotransmitter release probability, which was heterogeneous among synaptic boutons studied. For each neuron investigated, the liberation of neurotransmitters occurred through a predominant mechanism. The prevalence of multivesicular over univesicular release increased synaptic strength and enhanced short-term synaptic depression. These results show that the preference of hippocampal boutons to synchronously release one or more vesicles determines the strength and low pass filtering properties of the synapses established. |
| first_indexed | 2024-03-13T10:52:58Z |
| format | Article |
| id | doaj.art-0e8fd8fa52eb42fe88c4fda7e107015a |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-03-13T10:52:58Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-0e8fd8fa52eb42fe88c4fda7e107015a2023-05-17T05:44:15ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022023-05-011710.3389/fncel.2023.10572421057242Multivesicular release favors short term synaptic depression in hippocampal autapsesPablo Martínez San Segundo0Pablo Martínez San Segundo1Beatrice Terni2Beatrice Terni3Artur Llobet4Artur Llobet5Laboratory of Neurobiology, Department of Pathology and Experimental Therapy, Institute of Neurosciences, University of Barcelona, Barcelona, SpainBellvitge Biomedical Research Institute (IDIBELL), Barcelona, SpainLaboratory of Neurobiology, Department of Pathology and Experimental Therapy, Institute of Neurosciences, University of Barcelona, Barcelona, SpainBellvitge Biomedical Research Institute (IDIBELL), Barcelona, SpainLaboratory of Neurobiology, Department of Pathology and Experimental Therapy, Institute of Neurosciences, University of Barcelona, Barcelona, SpainBellvitge Biomedical Research Institute (IDIBELL), Barcelona, SpainPresynaptic terminals of the central nervous system can support univesicular and multivesicular synchronous release of neurotransmitters, however, the functional implications of the prevalence of one mechanism over the other are yet unresolved. Here, we took advantage of the expression of SF-iGluSnFR.S72A in the astrocytic feeder layer of autaptic hippocampal neuronal cultures to associate the liberation of glutamate to excitatory postsynaptic currents. The presence of the glutamate sensor in glial cells avoided any interference with the function of endogenous postsynaptic receptors. It was possible to optically detect changes in neurotransmitter release probability, which was heterogeneous among synaptic boutons studied. For each neuron investigated, the liberation of neurotransmitters occurred through a predominant mechanism. The prevalence of multivesicular over univesicular release increased synaptic strength and enhanced short-term synaptic depression. These results show that the preference of hippocampal boutons to synchronously release one or more vesicles determines the strength and low pass filtering properties of the synapses established.https://www.frontiersin.org/articles/10.3389/fncel.2023.1057242/fullpresynaptic terminalsynaptic vesicleneurotransmitter releaseshort-term synaptic depressionautapse |
| spellingShingle | Pablo Martínez San Segundo Pablo Martínez San Segundo Beatrice Terni Beatrice Terni Artur Llobet Artur Llobet Multivesicular release favors short term synaptic depression in hippocampal autapses Frontiers in Cellular Neuroscience presynaptic terminal synaptic vesicle neurotransmitter release short-term synaptic depression autapse |
| title | Multivesicular release favors short term synaptic depression in hippocampal autapses |
| title_full | Multivesicular release favors short term synaptic depression in hippocampal autapses |
| title_fullStr | Multivesicular release favors short term synaptic depression in hippocampal autapses |
| title_full_unstemmed | Multivesicular release favors short term synaptic depression in hippocampal autapses |
| title_short | Multivesicular release favors short term synaptic depression in hippocampal autapses |
| title_sort | multivesicular release favors short term synaptic depression in hippocampal autapses |
| topic | presynaptic terminal synaptic vesicle neurotransmitter release short-term synaptic depression autapse |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2023.1057242/full |
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