Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats
This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga, Faculty of Veterinary Medicine
2023-10-01
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| Series: | Jurnal Medik Veteriner |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/40017 |
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| author | Akhmad Afifudin Al-Anshori Anandia Nafisah Putri Alif Noviana Ismi Mohamad Kharis Suhud Hani Plumeriastuti Lilik Maslachah |
| author_facet | Akhmad Afifudin Al-Anshori Anandia Nafisah Putri Alif Noviana Ismi Mohamad Kharis Suhud Hani Plumeriastuti Lilik Maslachah |
| author_sort | Akhmad Afifudin Al-Anshori |
| collection | DOAJ |
| description | This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin. |
| first_indexed | 2024-03-11T16:47:53Z |
| format | Article |
| id | doaj.art-0e908dc2b47d4299837b945b63396529 |
| institution | Directory Open Access Journal |
| issn | 2615-7497 2581-012X |
| language | English |
| last_indexed | 2025-02-17T08:47:21Z |
| publishDate | 2023-10-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj.art-0e908dc2b47d4299837b945b633965292025-01-02T20:44:36ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2023-10-016214515410.20473/jmv.vol6.iss2.2023.145-15438088Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino RatsAkhmad Afifudin Al-Anshori0https://orcid.org/0000-0002-1798-7257Anandia Nafisah Putri1https://orcid.org/0000-0002-1331-4004Alif Noviana Ismi2https://orcid.org/0000-0003-2533-3920Mohamad Kharis Suhud3https://orcid.org/0000-0003-2635-9233Hani Plumeriastuti4https://orcid.org/0000-0002-4540-811XLilik Maslachah5https://orcid.org/0000-0002-5291-4678Postgraduate Program, Faculty of Veterinary Medicine, Universitas Airlangga, SurabayaMedical Study Program, Faculty of Medicine, Universitas Airlangga, SurabayaPharmacy Study Program, Faculty of Pharmacy, Universitas Airlangga, SurabayaPostgraduate Program, Faculty of Veterinary Medicine, Universitas Airlangga, SurabayaDepartment of Veterinary Science, Division of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, SurabayaDepartment of Veterinary Science, Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, SurabayaThis study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin.https://e-journal.unair.ac.id/JMV/article/view/40017ashitaba leavesburnsethosomal gelnanoparticleswound healing |
| spellingShingle | Akhmad Afifudin Al-Anshori Anandia Nafisah Putri Alif Noviana Ismi Mohamad Kharis Suhud Hani Plumeriastuti Lilik Maslachah Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats Jurnal Medik Veteriner ashitaba leaves burns ethosomal gel nanoparticles wound healing |
| title | Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats |
| title_full | Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats |
| title_fullStr | Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats |
| title_full_unstemmed | Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats |
| title_short | Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats |
| title_sort | efficacy of transdermal delivery nano ethosomal gel from ashitaba leaves on in vivo burn wound healing in albino rats |
| topic | ashitaba leaves burns ethosomal gel nanoparticles wound healing |
| url | https://e-journal.unair.ac.id/JMV/article/view/40017 |
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