Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats
ABSTRACT: To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections eve...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001824&lng=en&tlng=en |
| _version_ | 1798046297004965888 |
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| author | Betânia Souza Monteiro Roberta Delessa Faria Alessandra Rodrigues Coelho Zanella Emanuelle Pimentel Cruz Noely Pianca Godoi Wagner Alexey Back Fiorio Dominik Lenz Viviane Raposo Fortunato |
| author_facet | Betânia Souza Monteiro Roberta Delessa Faria Alessandra Rodrigues Coelho Zanella Emanuelle Pimentel Cruz Noely Pianca Godoi Wagner Alexey Back Fiorio Dominik Lenz Viviane Raposo Fortunato |
| author_sort | Betânia Souza Monteiro |
| collection | DOAJ |
| description | ABSTRACT: To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections every 24 hours, for 30 consecutive days and, in the Dexamethasone Group (DG), 30 rats were given 0.2mL subcutaneous dexamethasone (0.1mg kg-1) every 24 hours, for 30 consecutive days. After 30 days, all rats underwent surgery to create an experimental skin wound. The 30 animals of the DG group were divided into two equal groups, which received different treatments: the dexamethasone group (DG) received a single application of 0.5ml normal saline, via the intravenous route (IV), 48 hours after wound creation; and the Mesenchymal Stem Cells Dexamethasone group (MSCDG) received MSC transplantation at a concentration of 9x106 cells in a single IV application, 48 hours after wound creation. The surgical wounds of CG rats closed on average 14.75 days after creation and DG rats had wounds closed within 22 days; whereas, the surgical wounds of MSCDG rats were closed in 14 days. MSC infusion in dexamethasone-immunosuppressed patients contributed positively to epithelial healing in less time. |
| first_indexed | 2024-04-11T23:35:21Z |
| format | Article |
| id | doaj.art-0e916f2960ff4b9c8eb188638cee118b |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-11T23:35:21Z |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-0e916f2960ff4b9c8eb188638cee118b2022-12-22T03:56:57ZengUniversidade Federal de Santa MariaCiência Rural1678-459646101824182910.1590/0103-8478cr20151001S0103-84782016001001824Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar ratsBetânia Souza MonteiroRoberta Delessa FariaAlessandra Rodrigues Coelho ZanellaEmanuelle Pimentel CruzNoely Pianca GodoiWagner Alexey Back FiorioDominik LenzViviane Raposo FortunatoABSTRACT: To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections every 24 hours, for 30 consecutive days and, in the Dexamethasone Group (DG), 30 rats were given 0.2mL subcutaneous dexamethasone (0.1mg kg-1) every 24 hours, for 30 consecutive days. After 30 days, all rats underwent surgery to create an experimental skin wound. The 30 animals of the DG group were divided into two equal groups, which received different treatments: the dexamethasone group (DG) received a single application of 0.5ml normal saline, via the intravenous route (IV), 48 hours after wound creation; and the Mesenchymal Stem Cells Dexamethasone group (MSCDG) received MSC transplantation at a concentration of 9x106 cells in a single IV application, 48 hours after wound creation. The surgical wounds of CG rats closed on average 14.75 days after creation and DG rats had wounds closed within 22 days; whereas, the surgical wounds of MSCDG rats were closed in 14 days. MSC infusion in dexamethasone-immunosuppressed patients contributed positively to epithelial healing in less time.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001824&lng=en&tlng=entissue repaircell therapyimmunomodulation. |
| spellingShingle | Betânia Souza Monteiro Roberta Delessa Faria Alessandra Rodrigues Coelho Zanella Emanuelle Pimentel Cruz Noely Pianca Godoi Wagner Alexey Back Fiorio Dominik Lenz Viviane Raposo Fortunato Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats Ciência Rural tissue repair cell therapy immunomodulation. |
| title | Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats |
| title_full | Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats |
| title_fullStr | Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats |
| title_full_unstemmed | Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats |
| title_short | Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats |
| title_sort | mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats |
| topic | tissue repair cell therapy immunomodulation. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001824&lng=en&tlng=en |
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