An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells
Abstract The combination therapy which has been proposed as the strategy for the cancer treatment could achieve a synergistic effect for cancer therapies and reduce the dosage of the applied drugs. On account of the the unique properties as the high absorbed water content, biocompatibility, and flex...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-020-00764-6 |
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| author | Fatemeh Abedi Soodabeh Davaran Malak Hekmati Abolfazl Akbarzadeh Behzad Baradaran Sevil Vaghefi Moghaddam |
| author_facet | Fatemeh Abedi Soodabeh Davaran Malak Hekmati Abolfazl Akbarzadeh Behzad Baradaran Sevil Vaghefi Moghaddam |
| author_sort | Fatemeh Abedi |
| collection | DOAJ |
| description | Abstract The combination therapy which has been proposed as the strategy for the cancer treatment could achieve a synergistic effect for cancer therapies and reduce the dosage of the applied drugs. On account of the the unique properties as the high absorbed water content, biocompatibility, and flexibility, the targeting nanogels have been considred as a suitable platform. Herein, a non-toxic pH/thermo-responsive hydrogel P(NIPAAm-co-DMAEMA) was synthesized and characterized through the free-radical polymerization and expanded upon an easy process for the preparation of the smart responsive nanogels; that is, the nanogels were used for the efficient and controlled delivery of the anti-cancer drug doxorubicin (DOX) and chemosensitizer curcumin (CUR) simultaneously like a promising strategy for the cancer treatment. The size of the nanogels, which were made, was about 70 nm which is relatively optimal for the enhanced permeability and retention (EPR) effects. The DOX and CUR co-loaded nanocarriers were prepared by the high encapsulation efficiency (EE). It is important to mention that the controlled drug release behavior of the nanocarriers was also investigated. An enhanced ability of DOX and CUR-loaded nanoformulation to induce the cell apoptosis in the HT-29 colon cancer cells which represented the greater antitumor efficacy than the single-drug formulations or free drugs was resulted through the In vitro cytotoxicity. Overall, according to the data, the simultaneous delivery of the dual drugs through the fabricated nanogels could synergistically potentiate the antitumor effects on the colon cancer (CC). |
| first_indexed | 2024-04-14T05:25:00Z |
| format | Article |
| id | doaj.art-0e92a22d19034531828664e8b83725a1 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-14T05:25:00Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-0e92a22d19034531828664e8b83725a12022-12-22T02:10:01ZengBMCJournal of Nanobiotechnology1477-31552021-01-0119112210.1186/s12951-020-00764-6An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cellsFatemeh Abedi0Soodabeh Davaran1Malak Hekmati2Abolfazl Akbarzadeh3Behzad Baradaran4Sevil Vaghefi Moghaddam5Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad UniversityDrug Applied Research Center, Tabriz University of Medical SciencesDepartment of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad UniversityDepartment of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesDrug Applied Research Center, Tabriz University of Medical SciencesAbstract The combination therapy which has been proposed as the strategy for the cancer treatment could achieve a synergistic effect for cancer therapies and reduce the dosage of the applied drugs. On account of the the unique properties as the high absorbed water content, biocompatibility, and flexibility, the targeting nanogels have been considred as a suitable platform. Herein, a non-toxic pH/thermo-responsive hydrogel P(NIPAAm-co-DMAEMA) was synthesized and characterized through the free-radical polymerization and expanded upon an easy process for the preparation of the smart responsive nanogels; that is, the nanogels were used for the efficient and controlled delivery of the anti-cancer drug doxorubicin (DOX) and chemosensitizer curcumin (CUR) simultaneously like a promising strategy for the cancer treatment. The size of the nanogels, which were made, was about 70 nm which is relatively optimal for the enhanced permeability and retention (EPR) effects. The DOX and CUR co-loaded nanocarriers were prepared by the high encapsulation efficiency (EE). It is important to mention that the controlled drug release behavior of the nanocarriers was also investigated. An enhanced ability of DOX and CUR-loaded nanoformulation to induce the cell apoptosis in the HT-29 colon cancer cells which represented the greater antitumor efficacy than the single-drug formulations or free drugs was resulted through the In vitro cytotoxicity. Overall, according to the data, the simultaneous delivery of the dual drugs through the fabricated nanogels could synergistically potentiate the antitumor effects on the colon cancer (CC).https://doi.org/10.1186/s12951-020-00764-6pH/thermo-responsiveNanogelsDual-drug deliveryControlled releaseCombination therapy |
| spellingShingle | Fatemeh Abedi Soodabeh Davaran Malak Hekmati Abolfazl Akbarzadeh Behzad Baradaran Sevil Vaghefi Moghaddam An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells Journal of Nanobiotechnology pH/thermo-responsive Nanogels Dual-drug delivery Controlled release Combination therapy |
| title | An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells |
| title_full | An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells |
| title_fullStr | An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells |
| title_full_unstemmed | An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells |
| title_short | An improved method in fabrication of smart dual-responsive nanogels for controlled release of doxorubicin and curcumin in HT-29 colon cancer cells |
| title_sort | improved method in fabrication of smart dual responsive nanogels for controlled release of doxorubicin and curcumin in ht 29 colon cancer cells |
| topic | pH/thermo-responsive Nanogels Dual-drug delivery Controlled release Combination therapy |
| url | https://doi.org/10.1186/s12951-020-00764-6 |
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