MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance
Abstract Background MYB-CC transcription factors (TFs) genes have been demonstrated to be involved in the response to inorganic phosphate (Pi) starvation and regulate some Pi-starvation-inducible genes. However, their role in drought stress has not been investigated in bread wheat. In this study, th...
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1751-9 |
| _version_ | 1818318915980230656 |
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| author | Yaqing Li Shichang Zhang Nan Zhang Wenying Zhang Mengjun Li Binhui Liu Zhanliang Shi |
| author_facet | Yaqing Li Shichang Zhang Nan Zhang Wenying Zhang Mengjun Li Binhui Liu Zhanliang Shi |
| author_sort | Yaqing Li |
| collection | DOAJ |
| description | Abstract Background MYB-CC transcription factors (TFs) genes have been demonstrated to be involved in the response to inorganic phosphate (Pi) starvation and regulate some Pi-starvation-inducible genes. However, their role in drought stress has not been investigated in bread wheat. In this study, the TaMYBsm3 genes, including TaMYBsm3-A, TaMYBsm3-B, and TaMYBsm3-D, encoding MYB-CC TF proteins in bread wheat, were isolated to investigate the possible molecular mechanisms related to drought-tolerance in plants. Results TaMYBsm3-A, TaMYBsm3-B, and TaMYBsm3-D were mapped on chromosomes 6A, 6B, and 6D in wheat, respectively. TaMYBsm3 genes belonged to MYB-CC TFs, containing a conserved MYB DNA-binding domain and a conserved coiled–coil domain. TaMYBsm3-D was localized in the nucleus, and the N-terminal region was a transcriptional activation domain. TaMYBsm3 genes were ubiquitously expressed in different tissues of wheat, and especially highly expressed in the stamen and pistil. Under drought stress, transgenic plants exhibited milder wilting symptoms, higher germination rates, higher proline content, and lower MDA content comparing with the wild type plants. P5CS1, DREB2A, and RD29A had significantly higher expression in transgenic plants than in wild type plants. Conclusion TaMYBsm3-A, TaMYBsm3-B, and TaMYBsm3-D were associated with enhanced drought tolerance in bread wheat. Overexpression of TaMYBsm3-D increases the drought tolerance of transgenic Arabidopsis through up-regulating P5CS1, DREB2A, and RD29A. |
| first_indexed | 2024-12-13T10:00:49Z |
| format | Article |
| id | doaj.art-0e94528f011c4a8db9c7bfd0401a8493 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-13T10:00:49Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-0e94528f011c4a8db9c7bfd0401a84932022-12-21T23:51:40ZengBMCBMC Plant Biology1471-22292019-04-0119111110.1186/s12870-019-1751-9MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought toleranceYaqing Li0Shichang Zhang1Nan Zhang2Wenying Zhang3Mengjun Li4Binhui Liu5Zhanliang Shi6Shijiazhuang Academy of Agriculture and Forestry SciencesShijiazhuang Academy of Agriculture and Forestry SciencesShijiazhuang Academy of Agriculture and Forestry SciencesDryland Farming Institute, Hebei Academy of Agriculture and Forestry SciencesShijiazhuang Academy of Agriculture and Forestry SciencesDryland Farming Institute, Hebei Academy of Agriculture and Forestry SciencesShijiazhuang Academy of Agriculture and Forestry SciencesAbstract Background MYB-CC transcription factors (TFs) genes have been demonstrated to be involved in the response to inorganic phosphate (Pi) starvation and regulate some Pi-starvation-inducible genes. However, their role in drought stress has not been investigated in bread wheat. In this study, the TaMYBsm3 genes, including TaMYBsm3-A, TaMYBsm3-B, and TaMYBsm3-D, encoding MYB-CC TF proteins in bread wheat, were isolated to investigate the possible molecular mechanisms related to drought-tolerance in plants. Results TaMYBsm3-A, TaMYBsm3-B, and TaMYBsm3-D were mapped on chromosomes 6A, 6B, and 6D in wheat, respectively. TaMYBsm3 genes belonged to MYB-CC TFs, containing a conserved MYB DNA-binding domain and a conserved coiled–coil domain. TaMYBsm3-D was localized in the nucleus, and the N-terminal region was a transcriptional activation domain. TaMYBsm3 genes were ubiquitously expressed in different tissues of wheat, and especially highly expressed in the stamen and pistil. Under drought stress, transgenic plants exhibited milder wilting symptoms, higher germination rates, higher proline content, and lower MDA content comparing with the wild type plants. P5CS1, DREB2A, and RD29A had significantly higher expression in transgenic plants than in wild type plants. Conclusion TaMYBsm3-A, TaMYBsm3-B, and TaMYBsm3-D were associated with enhanced drought tolerance in bread wheat. Overexpression of TaMYBsm3-D increases the drought tolerance of transgenic Arabidopsis through up-regulating P5CS1, DREB2A, and RD29A.http://link.springer.com/article/10.1186/s12870-019-1751-9Drought stressTaMYBsm3Transcription factorTriticum aestivum LTaMYBsm3-D transgenic ArabidopsisValidation |
| spellingShingle | Yaqing Li Shichang Zhang Nan Zhang Wenying Zhang Mengjun Li Binhui Liu Zhanliang Shi MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance BMC Plant Biology Drought stress TaMYBsm3 Transcription factor Triticum aestivum L TaMYBsm3-D transgenic Arabidopsis Validation |
| title | MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance |
| title_full | MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance |
| title_fullStr | MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance |
| title_full_unstemmed | MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance |
| title_short | MYB-CC transcription factor, TaMYBsm3, cloned from wheat is involved in drought tolerance |
| title_sort | myb cc transcription factor tamybsm3 cloned from wheat is involved in drought tolerance |
| topic | Drought stress TaMYBsm3 Transcription factor Triticum aestivum L TaMYBsm3-D transgenic Arabidopsis Validation |
| url | http://link.springer.com/article/10.1186/s12870-019-1751-9 |
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