Multiplexed Profiling of Extracellular Vesicles for Biomarker Development
Abstract Extracellular vesicles (EVs) are cell-derived membranous particles that play a crucial role in molecular trafficking, intercellular transport and the egress of unwanted proteins. They have been implicated in many diseases including cancer and neurodegeneration. EVs are detected in all bodil...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2021-12-01
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| Series: | Nano-Micro Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s40820-021-00753-w |
| _version_ | 1831523055572090880 |
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| author | Cheng Jiang Ying Fu Guozhen Liu Bowen Shu Jason Davis George K. Tofaris |
| author_facet | Cheng Jiang Ying Fu Guozhen Liu Bowen Shu Jason Davis George K. Tofaris |
| author_sort | Cheng Jiang |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are cell-derived membranous particles that play a crucial role in molecular trafficking, intercellular transport and the egress of unwanted proteins. They have been implicated in many diseases including cancer and neurodegeneration. EVs are detected in all bodily fluids, and their protein and nucleic acid content offers a means of assessing the status of the cells from which they originated. As such, they provide opportunities in biomarker discovery for diagnosis, prognosis or the stratification of diseases as well as an objective monitoring of therapies. The simultaneous assaying of multiple EV-derived markers will be required for an impactful practical application, and multiplexing platforms have evolved with the potential to achieve this. Herein, we provide a comprehensive overview of the currently available multiplexing platforms for EV analysis, with a primary focus on miniaturized and integrated devices that offer potential step changes in analytical power, throughput and consistency. |
| first_indexed | 2024-12-14T08:53:55Z |
| format | Article |
| id | doaj.art-0e94b871b3eb4599b6e367ae0c7d8eb0 |
| institution | Directory Open Access Journal |
| issn | 2311-6706 2150-5551 |
| language | English |
| last_indexed | 2024-12-14T08:53:55Z |
| publishDate | 2021-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano-Micro Letters |
| spelling | doaj.art-0e94b871b3eb4599b6e367ae0c7d8eb02022-12-21T23:08:58ZengSpringerOpenNano-Micro Letters2311-67062150-55512021-12-0114113610.1007/s40820-021-00753-wMultiplexed Profiling of Extracellular Vesicles for Biomarker DevelopmentCheng Jiang0Ying Fu1Guozhen Liu2Bowen Shu3Jason Davis4George K. Tofaris5Nuffield Department of Clinical Neurosciences, New Biochemistry Building, University of OxfordDepartment of Chemistry, University of OxfordSchool of Life and Health Sciences, The Chinese University of Hong KongDermatology Hospital, Southern Medical UniversityDepartment of Chemistry, University of OxfordNuffield Department of Clinical Neurosciences, New Biochemistry Building, University of OxfordAbstract Extracellular vesicles (EVs) are cell-derived membranous particles that play a crucial role in molecular trafficking, intercellular transport and the egress of unwanted proteins. They have been implicated in many diseases including cancer and neurodegeneration. EVs are detected in all bodily fluids, and their protein and nucleic acid content offers a means of assessing the status of the cells from which they originated. As such, they provide opportunities in biomarker discovery for diagnosis, prognosis or the stratification of diseases as well as an objective monitoring of therapies. The simultaneous assaying of multiple EV-derived markers will be required for an impactful practical application, and multiplexing platforms have evolved with the potential to achieve this. Herein, we provide a comprehensive overview of the currently available multiplexing platforms for EV analysis, with a primary focus on miniaturized and integrated devices that offer potential step changes in analytical power, throughput and consistency.https://doi.org/10.1007/s40820-021-00753-wMultiplexed profilingExtracellular vesiclesExosomesLiquid biopsyPoint-of-careBiomarker |
| spellingShingle | Cheng Jiang Ying Fu Guozhen Liu Bowen Shu Jason Davis George K. Tofaris Multiplexed Profiling of Extracellular Vesicles for Biomarker Development Nano-Micro Letters Multiplexed profiling Extracellular vesicles Exosomes Liquid biopsy Point-of-care Biomarker |
| title | Multiplexed Profiling of Extracellular Vesicles for Biomarker Development |
| title_full | Multiplexed Profiling of Extracellular Vesicles for Biomarker Development |
| title_fullStr | Multiplexed Profiling of Extracellular Vesicles for Biomarker Development |
| title_full_unstemmed | Multiplexed Profiling of Extracellular Vesicles for Biomarker Development |
| title_short | Multiplexed Profiling of Extracellular Vesicles for Biomarker Development |
| title_sort | multiplexed profiling of extracellular vesicles for biomarker development |
| topic | Multiplexed profiling Extracellular vesicles Exosomes Liquid biopsy Point-of-care Biomarker |
| url | https://doi.org/10.1007/s40820-021-00753-w |
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